Suppressive effects of 1-[4-fluoro-2-(2-nitrovinyl)phenyl]pyrrolidine on the Toll-like receptor signaling pathways

Ahn, Soo Whan; Lim, Se Jin; Gu, Gyo-Jeong; Hong, Chae-Yeon; Kim, Ji-Soo; Jeong, Hyun Jung; Koh, Kwang Oh; Mang, Joo Yang; Kim, Dae Young; Youn, Hyung‐Sun

doi:10.1016/j.intimp.2014.10.033

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…Western blotting was performed as previously described (Ahn et al, 2015;Park, 2015). Equal amounts of cell extracts were subjected to 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the separated proteins were electrotransferred to a polyvinylidene difluoride membrane.…”

Section: Western Blotting Analysismentioning

confidence: 99%

“…Toll-like receptors (TLRs), one of the pathogen recognition receptors, detect structural components derived from invading microorganisms or damaged cells/tissues. The activation of TLRs triggers immune and inflammatory responses through the activation of cellular signal transduction and the expression of immune and inflammatory mediators (Ahn et al, 2015). TLR4 is the first characterized TLR and recognizes LPS, a cell wall component of Gram negative bacteria that causes systemic inflammatory sepsis.…”

Section: Eay Suppresses Lps-induced Cox-2 and Inos Expressionmentioning

confidence: 99%

“…TLR4 is the first characterized TLR and recognizes LPS, a cell wall component of Gram negative bacteria that causes systemic inflammatory sepsis. Recognition of LPS by TLR4 results in oligomerization of TLR4 and subsequent recruitment of the adaptor molecules, myeloid differentiation primary response gene 88 (MyD88) and Toll/Interleukin-1 receptor domain-containing adaptor-inducing interferon beta (TRIF) (Ahn et al, 2015). MyD88 recruits IL-1R-associated kinase (IRAK)-4 and IRAK-1, which further associate with TNFR-associated factor 6 (TRAF6), leading to the activation of NF-κB.…”

Section: Eay Suppresses Lps-induced Cox-2 and Inos Expressionmentioning

confidence: 99%

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Aster yomena suppresses LPS-induced cyclooxygenase-2 and inducible nitric oxide synthase expression

Kim

Shin

et al. 2016

Food and Agricultural Immunology

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Inflammation is a pathological process that is known to be involved in numerous diseases. Microbial infection or tissue injury activates inflammatory responses, resulting in the induction of proinflammatory proteins including cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Aster yomena is used in traditional Korean remedies to treat cough, asthma, and insect bites. Here, we investigated the effects of A. yomena extract (EAY) on the expression of COX-2 and iNOS induced by LPS. EAY inhibited NF-κB activation and IκBα degradation induced by LPS. EAY suppressed LPS-induced COX-2 and iNOS expression which are the target genes regulated through NF-κB activation in macrophages. EAY also suppressed LPS-induced nitrite production. These results suggest that EAY has the potential to be developed as a potent antiinflammatory drug.

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Section: Western Blotting Analysismentioning

confidence: 99%

Section: Eay Suppresses Lps-induced Cox-2 and Inos Expressionmentioning

confidence: 99%

Section: Eay Suppresses Lps-induced Cox-2 and Inos Expressionmentioning

confidence: 99%

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Aster yomena suppresses LPS-induced cyclooxygenase-2 and inducible nitric oxide synthase expression

Kim

Shin

et al. 2016

Food and Agricultural Immunology

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Suppression of Toll‐Like Receptor 4 Dimerization by 1‐[5‐Methoxy‐2‐(2‐nitrovinyl)phenyl]pyrrolidine

Ahn

Kim

et al. 2016

Archiv der Pharmazie

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Toll-like receptor 4 (TLR4) recognizes lipopolysaccharide (LPS) and triggers the activation of myeloid differention factor 88 (MyD88) and the Toll/interleukin-1 receptor domain-containing adapter, inducing interferon-β (TRIF)-dependent major downstream signaling pathways. To evaluate the therapeutic potential of 1-[5-methoxy-2-(2-nitrovinyl)phenyl]pyrrolidine (MNP), previously synthesized in our laboratory, its effect on signal transduction via the TLR signaling pathways was examined. Here, we investigated whether MNP modulates the TLR4 signaling pathways and which anti-inflammatory target in TLR4 signaling is regulated by MNP. MNP inhibited the activation of nuclear factor-κB (NF-κB) induced by LPS (TLR4 agonist), and it also inhibited the expression of cyclooxygenase-2 and inducible nitric oxide synthase. MNP inhibited LPS-induced NF-κB activation by targeting TLR4 dimerization in addition to IKKβ. These results suggest that MNP can modulate the TLR4 signaling pathway at the receptor level to decrease inflammatory gene expression.

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Eupatorium makinoisuppresses toll-like receptor signaling pathways

Ahn

Kim

Hong

et al. 2015

Food and Agricultural Immunology

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Suppressive effects of 1-[4-fluoro-2-(2-nitrovinyl)phenyl]pyrrolidine on the Toll-like receptor signaling pathways

Cited by 5 publications

References 31 publications

Aster yomena suppresses LPS-induced cyclooxygenase-2 and inducible nitric oxide synthase expression

Aster yomena suppresses LPS-induced cyclooxygenase-2 and inducible nitric oxide synthase expression

Suppression of Toll‐Like Receptor 4 Dimerization by 1‐[5‐Methoxy‐2‐(2‐nitrovinyl)phenyl]pyrrolidine

Eupatorium makinoisuppresses toll-like receptor signaling pathways

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