Suppressive Effects of Asbestos Exposure on the Human Immune Surveillance System

Nishimura, Yasumasa; Kumagai-Τakei, Naoko; Maeda, Megumi; Matsuzaki, Hidenori; Lee, Sai Peck; Yamamoto, Shoko; Hatayama, Tamayo; Yoshitome, Kei; Otsuki, Takemi

doi:10.1007/978-981-10-0351-6_1

Cited by 1 publication

(1 citation statement)

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“…Natural killer (NK) cell activity testing is one effective approach to determine the status of antitumor immunity in the body, which is reflected from the crosstalk between cancer cells and immune cells and the nature of those immune cells. NK cell activity is a conventional index which represents the ability of cell samples to injure NK cell-sensitive target cells, and we utilized the method of NK cell activity to evaluate the natural cytotoxic activity of peripheral blood mononuclear cells (PBMCs) obtained from patients and an NK cell line [1][2][3][4][5]. A recent 11-year follow-up study from 1986 to 1990 involving 3652 Japanese residents of the general population clearly demonstrated the importance of determining and evaluating NK cell activity.…”

Section: Introductionmentioning

confidence: 99%

A New Method to Determine Natural Killer Cell Activity Without Target Cells

Nishimura¹,

Kumagai-Τakei²,

Lee³

et al. 2017

Natural Killer Cells

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Natural killer (NK) cell activity is a conventional parameter used to determine the performance lytic activity against tumor as well as virus-infected cells in innate immunity. However, use of this parameter has several problems related to bioassay measurements. To measure NK cell activity, target cells and cell culture equipment are required and adequate pre-culture of target cells is needed to maintain constant sensitivity for NK cells. NK cell-activating receptors play an important role in the recognition of targets, which transduce the signals necessary for cellular machinery to induce target injury and cytokine production. We statistically examined the parameters related to the NK cell activity of human peripheral blood mononuclear cells (PBMCs) by multiple regression analysis, and obtained a formula with NK cell % and RNA levels of two genes in isolated NK cells. The score calculated using this formula with the three measured values showed significant correlation with NK cell activity. This prediction score, named the non-incubating natural killer (NINK) score, which is independent of target cells, is not affected by inappropriate preparation of those targets, and allows us to accurately compare the performance of NK cell activity among specimens.

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