Suppressive Mechanism of Salmosin, a Novel Disintegrin in B16 Melanoma Cell Metastasis

Kang, In Cheol; Kim, Doo Sik; Jang, Yangsoo; Chung, Kyeong Woo

doi:10.1006/bbrc.2000.3130

Cited by 77 publications

(47 citation statements)

References 16 publications

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“…The importance of αvβ3 integrin expression on tumoral cells for lung B16F10 cells invasion was confirmed by the blocking of this integrin using short synthetic RGD-based peptides 30 and by RGD-disintegrins. 31,32 Similar observations were performed for α4β1 integrin in this mouse model. 33,34 Our present paper is the first to report on the effect of antagonizing collagen receptors for melanoma metastasis.…”

Section: Discussionsupporting

confidence: 78%

“…A hematogenous metastasis model of B16F10 melanoma was developed based on the previously published method. 31 Briefly, B16F10 cells after trypsynization were suspended in 200 μl of PBS at a final density of 5 x 10 5 /ml. Snake venom proteins VP12 and/or viperistatin were added to cell suspensions at a concentration of 5 mg per kg of animal.…”

Section: Vp12 and Viperistatin Inhibit Melanoma Metastasismentioning

confidence: 99%

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Effect of VP12 and viperistatin on inhibition of collagen receptors: dependent melanoma metastasis

Staniszewska

Walsh²,

Rothman³

et al. 2009

Cancer Biology & Therapy

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Cancer Biology & Therapy 1507 VP12 and viperistatin inhibit melanoma metastasisViperistatin and VP12 isolated from Vipera paleastinae venom showed a potent inhibitory activity against collagen receptors, α1β1 and α2β1 integrins, respectively. Structurally, viperistatin belongs to the disintegrin family of proteins, whereas VP12 is composed of two subunits VP12A and VP12B displaying amino acid sequence homology with heterodimeric C-lectin type proteins. Viperistatin and VP12 used separately and simultaneously inhibited pro-metastatic activities of melanoma cells lines. The level of inhibition of MV3 and HS.939T human cell lines in cell adhesion and migration assays by both compounds was correlated with expression of α1β1 and α2β1 integrins on the cell surface. MV3 cells express collagen receptors to much higher extent than HS.939T and required the application of higher concentrations of inhibitors to block their adhesion to collagen types I and IV. A melanoma cell transmigration assay through a dHMVEC layer revealed that α1β1 integrin plays a significant role in invasion of HS.939T cells, while α2β1 integrin appears to be more important for MV3 cells. In an animal model of hematogenous metastasis of the mouse B16F10 cell line, the inhibitory effect of viperistatin and VP12 was only partial. These data suggest that collagen receptors may be an interesting target for development of new anti-metastatic therapies.

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Section: Discussionsupporting

confidence: 78%

Section: Vp12 and Viperistatin Inhibit Melanoma Metastasismentioning

confidence: 99%

Effect of VP12 and viperistatin on inhibition of collagen receptors: dependent melanoma metastasis

Staniszewska

Walsh²,

Rothman³

et al. 2009

Cancer Biology & Therapy

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“…These results suggest that HA and NMH induced the same oxidative stress and SIPS via similar (Kang et al 2000), induction of apoptosis (Chung et al 2003) Increase Apoptosis BCL2-associated X protein BAX Pro-apoptotic proteins (Adams and Cory 1998), mitochondrial dysfunction (Gross et al 1998 Activating transcription factor 3 ATF3 Maintaining DNA integrity and protecting against cell transformation (Yan et al 2005) Increase oxygen toxicities, because both conditions have the same oxygen partial pressure. Moreover, the expressions of proteins involved in heat shock and cell cycle check points were not significantly different between the two hyperoxic conditions.…”

Section: Discussionmentioning

confidence: 94%

Comparison of the effects of 40% oxygen and two atmospheric absolute air pressure conditions on stress-induced premature senescence of normal human diploid fibroblasts

Oh¹,

Lee²,

Lee³

et al. 2008

Cell Stress and Chaperones

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The pressure during hyperbaric oxygen treatment may increase oxygen toxicity via an augmented oxygen pressure in the gas. Nevertheless, only a few reports have been published on the effect of cells grown under 2 atmospheric absolute (ATA) pressure. To evaluate the effect of pressure on oxygen toxicity and to study effects in addition to oxygen toxicity, we designed an experiment to compare the effects of normobaric mild hyperoxia (NMH, 40% oxygen) and hyperbaric air condition (HA, air with 2 ATA) on human diploid fibroblasts (HDF) in a hyperbaric incubator. HDFs in both the NMH and the HA condition had a similar oxidative stress response and exhibited premature senescence. To investigate differences in gene profiling in cells grown in the NMH and HA conditions, samples from cells exposed to each condition were applied to microarrays. We found no expression difference in genes related to aging and deoxyribonucleic acid damage, but the expression of genes including cell adhesion, stress response, and transcription were significantly increased in fibroblasts that were responsive to pressure. Among 26 statistically reliable genes, the expression of apoptosis related genes such as ADAM22, Bax, BCL2L14, and UBD, as well as tumor suppressor-related genes like Axin2 and ATF, and also mitogen-activated protein kinase-related genes like mitogen-activated protein kinase kinase kinase 1, histamine receptor, and RAB24, were significantly changed in cells responsive to pressureinduced oxidative stress.

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“…It was found that RGD peptides were able to inhibit tumor metastasis and induce cell apoptosis. [16][17][18][19] Since then, peptides containing RGD motif have been extensively used in studies of cell adhesion, migration, growth and apoptosis via RGD-integrin interactions.…”

Section: Introductionmentioning

confidence: 99%

RGD-conjugated albumin nanoparticles as a novel delivery vehicle in pancreatic cancer therapy

Zhang

et al. 2012

Cancer Biology & Therapy

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These authors contributed equally to this work. Keywords: nanoparticles, albumin, RGD, FITC, delivery, pancreatic cancer, therapyIntegrin avb3 receptor is expressed on several types of cancer cells, including pancreatic cancer cells, and plays an important role in tumor growth and metastasis. The ability to target the integrin avb3 receptor on cancer cells increases the efficacy of targeted therapy and reduces the side effects. The aim of this study is to develop a novel arginine-glycineaspartic acid (RGD) peptide-conjugated albumin nanoparticle to enhance the intracellular uptake of anticancer drug into the pancreatic cancer cells through receptor-mediated endocytosis. In cellular uptake studies, the fluorescent signal of RGD-conjugated BSANPs in BxPC3 cells was higher than that of BSANPs without RGD conjugation as determined by fluorescence spectrophotometer. We also found that BSANPs bound to BxPC3 cells in a time-and concentration-dependent manner. The uptake of RGD-conjugated BSANPs by pancreatic cancer cells was inhibited by an excess amount of free RGD peptide, indicating that the binding and/or uptake were mediated by the avb3 receptor. Furthermore, the nanoparticles were found to be located close to the nuclei by using laser scanning confocal microscopy. Besides, no significant in vitro cytotoxicity was observed as measured with MTT assay. Both in vitro and in vivo antitumor efficacy was improved by targeting gemcitabine-loaded nanoparticles to BxPC-3 cells using RGD peptides. Therefore, the RGD-conjugated BSANPs hold great potential as an effective drug delivery system to deliver therapeutic agents to pancreatic cancer.

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Suppressive Mechanism of Salmosin, a Novel Disintegrin in B16 Melanoma Cell Metastasis

Cited by 77 publications

References 16 publications

Effect of VP12 and viperistatin on inhibition of collagen receptors: dependent melanoma metastasis

Effect of VP12 and viperistatin on inhibition of collagen receptors: dependent melanoma metastasis

Comparison of the effects of 40% oxygen and two atmospheric absolute air pressure conditions on stress-induced premature senescence of normal human diploid fibroblasts

RGD-conjugated albumin nanoparticles as a novel delivery vehicle in pancreatic cancer therapy

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