Suppressive Method for Unspecific Adsorption on a Semiconductor Electrode in the Specific Molecular Immobilization Process Using EC tag Method

Wakabayashi, Ryo; Haruyama, Tetsuya

doi:10.5796/electrochemistry.80.302

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…For this purpose, we developed the EC tag method 16 which can immobilize an organic molecule by attaching it to an electrode, including a semiconductor. The technique depends on electrochemical deposition using a coordination metal ion with an acyclic peptide.…”

Section: Introductionmentioning

confidence: 99%

Smooth Electron Transfer from a Photoexcited Dye to Semiconductor Electrode Through a Swingable Molecular Interface

et al. 2016

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The transfer of photoexcited electrons from a fluorescent dye molecule to a semiconductor electrode is regulated by the interfacial design between the electrode and the dye. In the case of larger functional molecules, the regulation becomes observable because of molecular steric hindrance on the solid electrode surface. We have studied the effectiveness of a "swingable" functional molecule layer on a solid substrate for immobilized catalysts and electrochemical reactions. The swingable molecular interface offers the possibility of a similar outcome in photoexcited current generation. The swingable design of the molecular layer may allow smooth current flow from the dye to the semiconductor electrode, because the dye molecules come in close proximity to the semiconductor surface through the swinging action of the linker molecules. In the present study, FTO (fluorinedoped tin oxide) electrodes were prepared with two types of dye-tagged molecular layers, differing only in the type of linker between the dye and the FTO surface: one with a flexible peptide linker capable of swinging back and forth and around, and the other with a rigid peptide linker. The two dye-sensitized FTO electrodes were assayed using photoexcited current measurements. As per the experimental results, the FTO electrode modified with the swingable linker molecule produced higher photoexcited current than that of the FTO modified with the rigid molecule linker.

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Section: Introductionmentioning

confidence: 99%

Smooth Electron Transfer from a Photoexcited Dye to Semiconductor Electrode Through a Swingable Molecular Interface

et al. 2016

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A “Swingable” straight-chain affinity molecule immobilized on a semi-conductor electrode for photo-excited current-based molecular sensing

Takatsuji

Wakabayashi

Sakakura

et al. 2015

Electrochimica Acta

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A B S T R A C TThe molecular affinities of biomolecules have found applications in the areas of clinical diagnostics, drug discovery, as well as allied fields of study. An affinity sensor is a unique in situ assay tool, which is valuable and convenient in practical situations. In this study, we designed a photo-excitable molecular interface with an affinity domain and fabricated with a "swingable" straight-chain affinity molecule immobilized on a semi-conductor electrode (SCE). The straight-chain affinity molecule possessed a photo-excitable dye at one end and was bound to the SCE surface at the other by the EC tag method, which was developed previously. A straight-chain molecule is too long to transfer electrons from the photo-excited dye to the conduction band of the SCE. However, the straight-chain molecule was designed with a "swing" structure, which made the transfer of electrons possible. The central region of the chain molecule has an affinity to the activated estrogen receptor (ER). When the activated ER bound to the affinity region, the molecular lost its swingable function, the electron transfer from the photo-excited dye to the SCE was consequently suppressed. Based on the unique swingable molecular interface, the affinity sensor can be used to determine the in situ concentration of endocrine disrupter ESTROGEN (17b-estradiol) at concentrations ranging from 2 to 10 nM with very good reproducibility. The superior assay reproducibility is responsible for the success of the EC tag method, which is a quantitative method for immobilizing molecules on SCE.

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Suppressive Method for Unspecific Adsorption on a Semiconductor Electrode in the Specific Molecular Immobilization Process Using EC tag Method

Cited by 2 publications

References 24 publications

Smooth Electron Transfer from a Photoexcited Dye to Semiconductor Electrode Through a Swingable Molecular Interface

Smooth Electron Transfer from a Photoexcited Dye to Semiconductor Electrode Through a Swingable Molecular Interface

A “Swingable” straight-chain affinity molecule immobilized on a semi-conductor electrode for photo-excited current-based molecular sensing

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