Supramolecular enzymatic systems of the dog blood: Clinical diagnostic implications

Zaitsev, S. Yu.; Maksimov, V. I.; Bardyukova, T. V.

doi:10.3103/s0027131408020119

Cited by 3 publications

(9 citation statements)

References 1 publication

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“…The elevated levels of the abovementioned enzymes in blood indicated that the liver should be further investigated (Maximov, Goudin & Lysov, 2010;Maximov et al, 2012;Zaitsev, 2017). The ALT or ACT isoenzyme profile can be especially useful for specific diagnosis, but it rather complicated and expansive for cattle and used mainly for dogs and cats (Zaitsev, Maksimov & Bardyukova, 2008). Any "malfunction" (mutation, overproduction, underproduction or deletion) of a single enzyme can also indicate a genetic disease.…”

Section: Serum Enzymesmentioning

confidence: 99%

“…and commonly used for animals (Kaneko, Harvey & Bruss, 2008;Zaitsev, 2017;Voronina, 2017). These tests are helpful to speed up the final diagnosis decision, as well as for planning and control of "therapeutic" treatment (Zaitsev, Maksimov & Bardyukova, 2008;Zaitsev, 2016;Voronina, 2017). Of course, a particular diagnosis in the case of severу diseases usually needs specific tests (Zaitsev, Maksimov & Bardyukova, 2008;Constable, Trefz & Stämpfli, 2019;Kaneko, Harvey & Bruss, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…These tests are helpful to speed up the final diagnosis decision, as well as for planning and control of "therapeutic" treatment (Zaitsev, Maksimov & Bardyukova, 2008;Zaitsev, 2016;Voronina, 2017). Of course, a particular diagnosis in the case of severу diseases usually needs specific tests (Zaitsev, Maksimov & Bardyukova, 2008;Constable, Trefz & Stämpfli, 2019;Kaneko, Harvey & Bruss, 2008). Interpretation of major biochemical parameters (MBP) usually is similar across major animal species, but there are a few peculiarities in each case, especially well-known for cattle.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical parameters, dynamic tensiometry and circulating nucleic acids for cattle blood analysis: a review

Zaitsev

Bogolyubova

Zhang

et al. 2020

PeerJ

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The animal’s blood is the most complicated and important biological liquid for veterinary medicine. In addition to standard methods that are always in use, recent technologies such as dynamic tensiometry (DT) of blood serum and PCR analysis of particular markers are in progress. The standard and modern biochemical tests are commonly used for general screening and, finally, complete diagnosis of animal health. Interpretation of major biochemical parameters is similar across animal species, but there are a few peculiarities in each case, especially well-known for cattle. The following directions are discussed here: hematological indicators; “total protein” and its fractions; some enzymes; major low-molecular metabolites (glucose, lipids, bilirubin, etc.); cations and anions. As example, the numerous correlations between DT data and biochemical parameters of cattle serum have been obtained and discussed. Changes in the cell-free nucleic acids (cfDNA) circulating in the blood have been studied and analyzed in a variety of conditions; for example, pregnancy, infectious and chronic diseases, and cancer. CfDNA can easily be detected using standard molecular biological techniques like DNA amplification and next-generation sequencing. The application of digital PCR even allows exact quantification of copy number variations which are for example important in prenatal diagnosis of chromosomal aberrations.

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Section: Serum Enzymesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biochemical parameters, dynamic tensiometry and circulating nucleic acids for cattle blood analysis: a review

Zaitsev

Bogolyubova

Zhang

et al. 2020

PeerJ

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“…Cells were cultivated and handled in the Institute of Bioorganic Chemistry, Russian Academy of Sciences: human breast carcinoma HBL-100, human keratinocytes HaCaT, human epithelial carcinomas A431 and canine kidney epithelial cells MDCK. Primary cell cultures of human lymphocytes were isolated by standard procedure [ 18 – 21 ]. Cell trackers: MitoTracker Green FM and Hoechst 33342, were purchased from Invitrogen and Sigma-Aldrich, respectively.…”

Section: Methodsmentioning

confidence: 99%

Conjugates of a Photoactivated Rhodamine with Biopolymers for Cell Staining

Zaitsev

Shaposhnikov

Solovyeva

et al. 2014

The Scientific World Journal

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Conjugates of the photoactivated rhodamine dyes with biopolymers (proteins, polysaccharides, and nucleic acids) are important tools for microscopic investigation of biological tissue. In this study, a precursor of the photoactivated fluorescent dye (PFD) has been successfully used for staining of numerous mammalian cells lines and for conjugate formation with chitosan (“Chitosan-PFD”) and histone H1 (“Histone H1.3-PFD”). The intensive fluorescence has been observed after photoactivation of these conjugates inside cells (A431, HaCaT, HEK239, HBL-100, and MDCK). Developed procedures and obtained data are important for further application of novel precursors of fluorescent dyes (“caged” dyes) for microscopic probing of biological objects. Thus, the synthesized “Chitosan-PFD” and “Histone H1-PFD” have been successfully applied in this study for intracellular transport visualization by fluorescent microscopy.

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“…Such membrane nanostructures have already been used in various fields of chemistry, physics, biology, and medicine [3,4,5,6,7,8,9,10,11,12]. The latest achievements in this area are associated with the inception and fast development of a new sphere of interdisciplinary science—nanotechnology [1,2,3].…”

Section: Introductionmentioning

confidence: 99%

Membrane and Films Based on Novel Crown-Containing Dyes as Promising Chemosensoring Materials

2010

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This paper discusses several works on supramolecular systems such as monolayer and multilayer, polymer films of various crown-containing dyes, surface-active monomers and polymers. Design, production and investigation of the membrane nanostructures based on crown ethers is a rapidly developing field at the “junction” of materials sciences and nanotechnology. These nanostructures can serve as convenient models for studying the self-organization and molecular recognition processes at interfaces that are typical for biomembranes. Based on the results obtained for such structures by absorption and fluorescence spectroscopy, atomic force and Brewster-angle microscopy, surface pressure and surface potential isotherm measurements, the possibility of developing micro- and nanomaterials possessing a set of specified properties (including chemosensor, photochromic and photorefractive materials) is demonstrated.

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Supramolecular enzymatic systems of the dog blood: Clinical diagnostic implications

Cited by 3 publications

References 1 publication

Biochemical parameters, dynamic tensiometry and circulating nucleic acids for cattle blood analysis: a review

Biochemical parameters, dynamic tensiometry and circulating nucleic acids for cattle blood analysis: a review

Conjugates of a Photoactivated Rhodamine with Biopolymers for Cell Staining

Membrane and Films Based on Novel Crown-Containing Dyes as Promising Chemosensoring Materials

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