Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein

Abstract

This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Scheme 1. Schematic illustration of STA constructed by GC5A-6C · Fl reporter pair to detect PLP.

Monitoring Methionine Decarboxylase by a Supramolecular Tandem Assay

“…The enzymatic activity could be monitored by the detectable fluorescence response [19] . STA represents advantages of being fast, affordable, and remarkably simple, which has demonstrated wide applications in enzymatic activity monitoring, inhibitor and activator screening, [20] sensor array, [21] and membrane transport [22] …”

“…The enzymatic activity could be monitored by the detectable fluorescence response. [19] STA represents advantages of being fast, affordable, and remarkably simple, which has demonstrated wide applications in enzymatic activity monitoring, inhibitor and activator screening, [20] sensor array, [21] and membrane transport. [22] Supramolecular hosts are a family of well-designed artificial receptors with a discrete cavity that provides efficient hostguest interactions to certain biological substances.…”

Cascade Detection of Pyridoxal 5′-Phosphate and Al3+ Ions Based on Dual-Functionalized Red-Emitting Copper Nanoclusters

“…30 The recent study revealed that cofactor PLP showed the higher inclusion complexing ability with β-CD than the cofactor PY. 39 In addition, the recorded FTIR spectra of β-CD-GSH-CuNCs in the presence and absence of PLP are almost similar (Figure S6), which supported the inclusion of PLP in the hydrophobic inner cavity of β-CD capped over β-CD-GSH-CuNCs. Therefore, as there is no spectral overlap between the fluorescence spectrum of β-CD-GSH-CuNCs and the absorption spectrum of PLP, the photoinduced electron transfer from PLP to the excited NCs along with the copresence of three ligands (GSH, β-CD, and PLP) over the NCs is expected to perturb the fluorescence profile of β-CD-GSH-CuNCs.…”

“…The cavity of the functionalized β-CD is suitable to encapsulate the VB 6 cofactor PLP and formed the inclusion complex β-CD/PLP through multiple hydrogen bondings . The recent study revealed that cofactor PLP showed the higher inclusion complexing ability with β-CD than the cofactor PY . In addition, the recorded FTIR spectra of β-CD-GSH-CuNCs in the presence and absence of PLP are almost similar (Figure S6), which supported the inclusion of PLP in the hydrophobic inner cavity of β-CD capped over β-CD-GSH-CuNCs.…”

Recent advances in macrocyclic arenes-based fluorescent indicator displacement assays

“…The GC5A and FI reporter pair was also chosen to detect turn-on fluorescence in enzymatic substrate Pyridoxal-5′-phosphate ( PLP ) through F-IDA. ( Yue et al, 2019 ).…”