Supramolecularly Multicolor DNA Decoding Using an Indicator Competition Assay

Abstract

Scheme 1. ICA Rationale (A) Proposed in This Work and the Previously Developed Rationales of IDA (B), ABA (C), and DBA (D) a a For (D), with analyte also serving as indicator. I: indicator; A: analyte. Letter pubs.acs.org/ac

Identification of Ferric Ions Using a Palmatine@Q[8] Fluorescent Probe

“…Palmatine (PAL) is a clinically important natural isoquinoline alkaloid that has attracted much attention due to various biochemical and pharmacological effects, PAL is also fluorescent guest that can interact with water‐soluble Q[7], and various research groups have employed the PAL@Q[7] inclusion complex as a fluorescence probe to determine organic compounds, including drugs,‐ amino acids, pesticide, and for use in biological monitoring and preservatives . Recent research demonstrated that the PAL@Q[7] inclusion complex can be used for supramolecular multicolor DNA decoding . However, inclusion complexes between PAL and Q[n]s other than Q[7] have not yet been reported.…”

“…Although the interaction between Q[7] and PAL are well characterised,‐ interaction between Q[8] and PAL has not been demonstrated, presumably due to the limited water solubility of Q[8]. However, we found that in neutral water, interaction between Q[8] and PAL not only improved the water‐solubility of Q[8], but was also accompanied by fluorescence enhancement of PAL.…”

Cucurbiturils in nucleic acids research

“…53,54 In 2018, Y. Shao and co-workers described a supramolecular multicolor DNA sensor for the selective recognition of abasic DNA sites based on the ICA concept with CB [7]. 55 Two natural alkaloids, namely coptisine (21) and palmatine (22) have been used as fluorescent indicators for AP DNA sites (Scheme 3). Both alkaloids form 1 : 1 inclusion complexes with CB [7] that is accompanied by pronounced increase of their fluorescence.…”

DNA-supramolecule conjugates in theranostics

“…The strategy efficiently enhanced the sensitivity of the split aptamer-based sensor. Moreover, the strategy could be extended to multiplexed analysis based on the diversity of available responsive dye-macrocycle systems 76,77.…”