Surface Active Agents and Their Application in Bacteriology

Glassman, Harold N.

doi:10.1128/br.12.2.105-148.1948

Cited by 116 publications

(10 citation statements)

References 179 publications

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“…10, 2005 (Kabara, 1978). Glassman (1949) reported that proteins reduce the bactericidal activity of surface-active agents like monoglycerides due to the formation of lipid-protein complexes. However, results of this study indicate Surviving bacterial populations (expressed in log cfu/mL) in milk samples stored at 39°C for 24 h were enumerated at specified time points on tryptic soy agar plates containing 0.6% yeast extract.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Effect of Caprylic Acid and Monocaprylin on Major Bacterial Mastitis Pathogens

Nair

Joy

Vasudevan

et al. 2005

Journal of Dairy Science

141

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Bovine mastitis is the most significant economic drain on the worldwide dairy industry. Concerns regarding poor cure rates, emergence of bacterial resistance, and residues in milk necessitate development of alternative therapeutic approaches to antibiotics for treatment of mastitis. A variety of free fatty acids and their monoglycerides have been reported to exert antimicrobial activity against a wide range of microorganisms. The objective of our study was to examine the efficacy of caprylic acid, a short-chain fatty acid, and its monoglyceride, monocaprylin, to inactivate common mastitis pathogens, including Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Staphylococcus aureus, and Escherichia coli. Milk samples containing 50 mM or 100 mM caprylic acid, and 25 mM or 50 mM monocaprylin were inoculated separately with a 3-isolate mixture of each of the 5 pathogens, and incubated at 39 degrees C. Populations of surviving bacteria were determined at 0 min, 1 min, 6 h, 12 h, and 24 h of incubation. Both caprylic acid and monocaprylin reduced all 5 pathogens by >5.0 log cfu/mL after 6 h of incubation. Among the bacterial species tested, Strep. agalactiae, Strep. dysgalactiae, and Strep. uberis were most sensitive, and E. coli was most tolerant to caprylic acid and monocaprylin. Results of this study indicate that caprylic acid and monocaprylin should be evaluated as alternatives or adjuncts to antibiotics as intra-mammary infusion to treat bovine mastitis.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Effect of Caprylic Acid and Monocaprylin on Major Bacterial Mastitis Pathogens

Nair

Joy

Vasudevan

et al. 2005

Journal of Dairy Science

141

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“…The use of continuous shaking in Evelyn tubes during incubation permits assays in 20 to 24 hours. Inclusion of Tween 80 in bacterial media was reported by Dubos and Davis (4) to enhance the rate and abundance of growth of tubercle bacilli and has been applied extensively with other organisms (6). The stimulatory effect of the lactate is similar to that observed by Tosic (15), who attributed it to the buffering effect of the bicarbonate formed from the lactate during growth of Acetobacter turbidans.…”

Section: Discussionmentioning

confidence: 55%

Microbiological Assay of Panthenol

Ritter¹,

Rubin²

1949

Anal. Chem.

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“…Interestingly, regardless of the similar relative percentages of the aforementioned chemical constituents of the secretions, three gram-positive (B. cereus, S. aureus, and L. monocytogenes) and three gram-negative bacterial strains (E. coli, S. typhimurium, and P. aeruginosa) varied in their susceptibility to all the tested pygidial gland secretions (Nenadić et al, 2016a;Vesović et al, 2022), but also to methacrylic and angelic acids (Vesović et al, 2022). Although it has been previously revealed that organic acids, such as formic, acetic, and other short-chain fatty acids, could be associated with antimicrobial activity (Glassman, 1948;Kabara et al, 1972;Desbois and Smith, 2010;Raftari et al, 2012;Nenadić et al, 2016a,b;Nenadić, 2019), there is a particular lack of data on the antimicrobial properties of methacrylic and angelic acids. It has been determined that angelic acid is a potent antioxidant (Varsha et al, 2015;VasudhaUdupa et al, 2021) with assumed antimicrobial potential (Vesović et al, 2022).…”

Section: Antimicrobial Surveymentioning

confidence: 99%

The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)

et al. 2023

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The pygidial glands of carabids produce strong-smelling vapours. In this study, we examined the chemical composition of the gland secretions and the structure of the glands in five species of Carabini ground beetles (one species from the subtribe Calosomatina and four species from the subtribe Carabina): Calosoma (Calosoma) maderae (Fabricius, 1775), Carabus (Carabus) granulatus Linnaeus, 1758, C. (Limnocarabus) clathratus Linnaeus, 1760, C. (Carabus) ulrichii Germar, 1823, and C. (Procerus) gigas Creutzer, 1799. Additionally, we tested the antibacterial potential of the pygidial gland secretions of the two latter species against 11 bacterial strains. In order to detect the chemical content of the secretions, we used gas chromatography–mass spectrometry (GC–MS). The secretion extracts were applied against selected strains of medically important bacteria. We used bright-field microscopy to examine the morphology of the glands. We discovered a total of 11 chemical compounds in the pygidial gland extracts of the ground beetles we analysed. Ten of these compounds were identified as seven carboxylic acids, two hydrocarbons, and one aromatic aldehyde, while one chemical remained unidentified. Most of the components were isolated from the secretion of C. (L.) clathratus (nine), while the lowest number of compounds was found in C. (P.) gigas (two). Methacrylic acid was the most dominant compound by percentage in all five species, while angelic acid was also detected in all samples. As expected, salicylaldehyde was exclusively found in the species of the genus Calosoma Weber, 1801. The secretion of C. (P.) gigas was shown to achieve the highest level of antibacterial activity against Pseudomonas aeruginosa, Salmonella enterica, and S. typhimurium (even the same level as the positive control streptomycin), while the secretion of C. (C.) ulrichii achieved the highest antimicrobial potential against Staphylococcus epidermidis, S. aureus, Listeria monocytogenes, and Bacillus cereus. The most noticeable difference in the structure of the glands between the two genera is that the reservoir in Calosoma is more significantly narrowed as it leads into the efferent duct, compared to that of Carabus.

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Surface Active Agents and Their Application in Bacteriology

Cited by 116 publications

References 179 publications

Antibacterial Effect of Caprylic Acid and Monocaprylin on Major Bacterial Mastitis Pathogens

Antibacterial Effect of Caprylic Acid and Monocaprylin on Major Bacterial Mastitis Pathogens

Microbiological Assay of Panthenol

The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)

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