Surface adhesins of lactobacilli loosely connected to the cell wall and eliminated into the environment during culturing in liquid nutrient media

Anokhina, I. V.; Кравцов, Э. Г.; Yashina, N. V.; Ermolaev, A. V.; Chesnokova, V. L.; Далин, М. В.

doi:10.1007/s10517-006-0431-6

Cited by 4 publications

(12 citation statements)

References 7 publications

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“…The first group is composed of proteins that contain a signal peptide, which is located in the N-terminal part of the sequence to direct the protein to the protein export machinery. The second group includes surface-associated proteins that are simply shed from the bacteria due to the normal turnover of the cell wall (Anokhina et al, 2006b;Turner et al, 2004;Driessen & Nouwen, 2008;Sánchez et al, 2009b). Secretion systems are highly conserved among eubacteria, given the high degree of homology among the genes coding for their monomeric components (van Wely et al, 2001).…”

Section: Proteins Secreted By Probiotic Bacteriamentioning

confidence: 99%

Extracellular proteins secreted by probiotic bacteria as mediators of effects that promote mucosa–bacteria interactions

2010

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During the last few years, a substantial body of scientific evidence has accumulated suggesting that certain surface-associated and extracellular components produced by probiotic bacteria could be responsible for some of their mechanisms of action. These bacterial components would be able to directly interact with the host mucosal cells; they include exopolysaccharides, bacteriocins, lipoteichoic acids and surface-associated and extracellular proteins. Extracellular proteins include proteins that are actively transported to the bacterial surroundings through the cytoplasmic membrane, as well as those that are simply shed from the bacterial surface. Compared to the other bacterial components, the interactive ability of extracellular proteins/ peptides has been less extensively studied. In this review, current findings supporting an interaction between extracellular proteins/peptides produced by probiotic bacteria (strains of the genera Bifidobacterium, Lactobacillus and Escherichia) and host mucosal cells are discussed. Research needs and future trends are also considered.

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Section: Proteins Secreted By Probiotic Bacteriamentioning

confidence: 99%

Extracellular proteins secreted by probiotic bacteria as mediators of effects that promote mucosa–bacteria interactions

2010

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“…Cultures identified as C. albicans in Nickerson chromogenic medium (HiMedia) with parallel induction of the germinative tubule growth in the presence of the serum were selected for the experiment. C. albicans strains for further experiments were cultured in Saburo broth at 37 o C for 24 h [1].…”

Section: Methodsmentioning

confidence: 99%

“…However, after preincubation of these cells with Lactobacillus strains freshly isolated from the vaginas of clinically healthy women, adhesion of C. albicans to the target cells decreases by 30-40%. The use of commercial lactobacterial preparations (acylact or lactobacterin) for the same purpose is little effective, because these preparations are based on strains of enteric origin; being introduced into the vagina, these probiotic lactobacillus cultures (not characteristic of this biotope) almost loose their capacity to adhere to VE and colonize them.We previously showed that L. fermentum strain 90 TS-4 (21) expresses adhesin capable of detaching from the cell surface into the culture medium [1]. The components of the resultant culture fluid (CF) binding to VE membrane receptors compete with adhesins of other bacteria preventing their colonization on the mucosa.…”

mentioning

confidence: 99%

“…We previously showed that L. fermentum strain 90 TS-4 (21) expresses adhesin capable of detaching from the cell surface into the culture medium [1]. The components of the resultant culture fluid (CF) binding to VE membrane receptors compete with adhesins of other bacteria preventing their colonization on the mucosa.…”

mentioning

confidence: 99%

“…The components of the resultant culture fluid (CF) binding to VE membrane receptors compete with adhesins of other bacteria preventing their colonization on the mucosa. Clone 3 of L. fermentum strain 90 TS-4 (21) proved to be the most active producer of the component inhibiting adhesion of C. albicans reference strain, but not modifying adhesion of FimH-positive E. coli M17 strain [1].…”

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confidence: 99%

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Bactericidal activity of culture fluid components of Lactobacillus fermentum strain 90 TS-4 (21) clone 3, and their capacity to modulate adhesion of Candida Albicans yeast-like fungi to vaginal epithelial cells

et al. 2007

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Antagonistic activities of L. fermentum strain 90 TS-4 (21), L. casei ATCC 27216, and L. acidophilus ATCC 4356 and bactericidal activity of lactobacillus culture fluid towards E. coli strain K12, S. aureus, and S. epidermidis test cultures were studied. The bactericidal effect of L. fermentum strain 90 TS-4 (21) clone 3 culture fluid preparation (pH 6.0) on the test cultures was dose-dependent. Adhesion of C. albicans yeast-like fungi to vaginal epitheliocytes was more pronounced for strains isolated from women with asymptomatic infection than for strains isolated from women with manifest forms. L. fermentum strain 90 TS-4 (21) clone 3 culture fluid preparation modulated adhesion of yeast-like fungi only if the fungal strain was initially highly adherent.

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Adhesion characteristics of lactobacillus is a criterion of the probiotic choice

et al. 2008

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A sampling of lactobacilli from the German National Collection of Microorganisms and L. fermentum 90 TS-4 (21) reference strain clone 3 (Russian Federation) were studied. The results indicate that the receptors on the surface of lactobacillus strains from the German collection had no structures complementary to type 1 fimbriae, though adhesins of some of them reacted with mannose and galactose receptors. Adhesion on a monolayer of continuous cell cultures showed that adhesion activity of lactobacilli was a function of many derivatives, and hence, the choice of a model for evaluation of the adhesion characteristics of the strain should be based on adhesins exhibiting universal properties in different test systems. One of them can be lectin-binding adhesin; its expression on the surface of cultures of lactobacilli from the German collection varies within the same range as was shown previously for lactobacilli, studied by the same criterion. The molecular weight of lectin-binding adhesin is 25-30 kDa, and the corresponding receptors are frequently present on various eukaryotic cells, and hence, cell models can be considered as the most adequate for studies of the competitive interactions between lactobacilli and adhesins of pathogenic microorganisms.

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Surface adhesins of lactobacilli loosely connected to the cell wall and eliminated into the environment during culturing in liquid nutrient media

Cited by 4 publications

References 7 publications

Extracellular proteins secreted by probiotic bacteria as mediators of effects that promote mucosa–bacteria interactions

Extracellular proteins secreted by probiotic bacteria as mediators of effects that promote mucosa–bacteria interactions

Bactericidal activity of culture fluid components of Lactobacillus fermentum strain 90 TS-4 (21) clone 3, and their capacity to modulate adhesion of Candida Albicans yeast-like fungi to vaginal epithelial cells

Adhesion characteristics of lactobacillus is a criterion of the probiotic choice

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