Bactericidal activity of culture fluid components of Lactobacillus fermentum strain 90 TS-4 (21) clone 3, and their capacity to modulate adhesion of Candida Albicans yeast-like fungi to vaginal epithelial cells

Anokhina, I. V.; Кравцов, Э. Г.; Проценко, А. В.; Yashina, N. V.; Yermolaev, A V; Chesnokova, V. L.; Далин, М. В.

doi:10.1007/s10517-007-0112-0

Cited by 8 publications

(7 citation statements)

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“…Intriguingly, the EPS resulted to be a mannan polysaccharide possessing a structure very similar to the one produced by Candida albicans [23]. The EPS was found to be present in adherent biofilms and might be involved in initial and permanent adhesion.…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus crispatus L1: high cell density cultivation and exopolysaccharide structure characterization to highlight potentially beneficial effects against vaginal pathogens

et al. 2014

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BackgroundVaginal lactic acid bacteria defend the host against pathogens through a combination of competitive exclusion, competition for nutrients, production of antimicrobial substances and through the activation of the immune system. A new human isolate named Lactobacillus crispatus L1 was characterized in this work, and a preliminary evaluation of its probiotic potential is described together with a process to obtain a high productivity of viable biomass.ResultsIn a simulated digestion process 1.8⋅1010 cells∙ml−1 survived the gastric environment with 80% viability, without being affected by small intestine juices. Experiments on six different C sources were performed to analyze growth and organic acids production and, glucose, provided the best performances. A microfiltration strategy was exploited to improve the cellular yield in 2 L-fermentation processes, reaching 27 g · l−1 of dry biomass. Moreover, L. crispatus L1 demonstrated a greater stability to high concentrations of lactic acid, compared to other lactobacilli. The specific L. crispatus L1 exopolysaccharide was purified from the fermentation broth and characterized by NMR showing structural features and similarity to exopolysaccharides produced by pathogenic strains. Live L. crispatus L1 cells strongly reduced adhesion of a yeast pathogenic strain, Candida albicans in particular, in adherence assays. Interestingly a higher expression of the human defensin HBD-2 was also observed in vaginal cells treated with the purified exopolysaccharide, indicating a possible correlation with C. albicans growth inhibition.ConclusionsThe paper describes the evaluation of L. crispatus L1 as potential vaginal probiotic and the fermentation processes to obtain high concentrations of viable cells.

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Section: Introductionmentioning

confidence: 99%

Lactobacillus crispatus L1: high cell density cultivation and exopolysaccharide structure characterization to highlight potentially beneficial effects against vaginal pathogens

et al. 2014

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“…albicans fungi were cultured in Saburo liquid and solid media (HiMedia) at 37 o C during 16-18 h [1].…”

Section: Methodsmentioning

confidence: 99%

“…The experiment was carried out 3 times according to our method described previously [1]. The results were evaluated by the paired correlation method using Microsoft Excel 2010 statistical functions.…”

Section: Methodsmentioning

confidence: 99%

Effects of Female Sex Hormones on Adhesion of Candida Albicans Yeast-Like Fungi to the Buccal Epithelium

et al. 2014

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Hormonal regulation of receptor expression and glycogen concentrations in the epithelial cells of the oral cavity remains poorly studied. Adhesion of microorganisms to the vaginal epithelium correlates with their adhesion to the buccal epithelium. Analysis of the correlation between Candida adhesion to the vaginal and buccal epithelium depending on the hormonal status has demonstrated that activity of Candida albicans strains in the buccal epithelium correlates with their adhesion activity in the vaginal epithelium, with the coefficient of correlations (r) reaching 0.76.

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“…In addition, LAB have been found to show antifungal activity. In this respect, Lactobacillus casei and Lactobacillus acidophilus possess good antifungal properties and are able to protect immunocompromised people from opportunistic infections and adhesion by Candida albicans or other Candida species as described by many researchers in their works (Anokhina et al, 2007). A strong antifungal activity of cell-free supernatants from L. casei subsp.…”

Section: Introductionmentioning

confidence: 95%

Organic Acids Production and Antagonistic Effect of Some Strains of Probiotics

Hauka

Selim

El-Sawah

et al. 2014

Journal of Agricultural Chemistry and Biotechnology

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Organic acids production and antimicrobial activities of Lactobacillus acidophilus KF724889, Lactobacillus casei KF724890 and Streptococcus thermophilus KF724886, KF724887 and KF724888 strains, which isolated from dairy products as probiotics were screened. Eleven organic acids were detected in the different LAB filtrates, acetic, ascorbic, citric, formic, oxalic, malic, maleic, lactic, propionic, butyric and succinic acids. lactic and acetic acids were the major acids produced by the five strains. Lactobacillus in general and L. acidophilus in especial were the most active in acids production. Both L. acidophilus and L. casei produced the highest quantity of lactic acid, being 3257.4 and 2447.75 mg/100 ml, respectively, while Str. thermophilus strains KF724886, KF724887 and KF724888 produced 1613.36, 1964.52 and 2031.131 mg/100ml, respectively. Formic acid did not produce by Str. thermophilus KF724886. Supernatants obtained from the tested bacteria exhibited varying degrees of inhibitory effect against indicators pathogenic bacteria and yeast. All the tested bacteria have antagonistic effect against Staphylococcus aureus, Pseudomonas aeruginosa and Protus vulgaris. Among the isolates, L. acidophilus was the most effective strain for inhibiting pathogens growth with strong inhibitory effect against P. aeruginosa, L. monocytogenes and Candida albicans. Only L. acidophilus and Str. thermophilus KF724888 caused inhibitory effect against Bacillus cereus, L. monocytogenes and C. albicans. L. casei, Str. thermophilus KF724886 and Str. thermophilus KF724887 failed in inhibiting growth of E. coli, B. cereus, L. monocytogenes and C. albicans. L. acidophilus inhibited growth of E. coli, while Str. thermophilus KF724888 failed. The results showed that all cell free supernatants (CFSs) of LAB cultures have ability to inhibit all the tested foodcontaminating fungi. Based on dry weight measurements of fungal biomass, CFSs of L. acidophilus showed high antifungal activity against the tested fungi. CFSs of Str. thermophilus KF724887 strain showed strong inhibition percentages against growth of A. niger, T. harzianum, P. chrysogenum and A. pullulans (56.63, 54.84, 52.92 and 38.88%, respectively). The study revealed that lactic acid bacteria isolated from Egyptian fermented milk, are capable of producing organic acids and antimicrobial substances which have antagonistic effect on pathogenic organisms, thus, may be promising sources of preservative that may in future be applied to food.

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Bactericidal activity of culture fluid components of Lactobacillus fermentum strain 90 TS-4 (21) clone 3, and their capacity to modulate adhesion of Candida Albicans yeast-like fungi to vaginal epithelial cells

Cited by 8 publications

References 1 publication

Lactobacillus crispatus L1: high cell density cultivation and exopolysaccharide structure characterization to highlight potentially beneficial effects against vaginal pathogens

Lactobacillus crispatus L1: high cell density cultivation and exopolysaccharide structure characterization to highlight potentially beneficial effects against vaginal pathogens

Effects of Female Sex Hormones on Adhesion of Candida Albicans Yeast-Like Fungi to the Buccal Epithelium

Organic Acids Production and Antagonistic Effect of Some Strains of Probiotics

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