Surface-adhesive and osteogenic self-assembled peptide nanofibers for bioinspired functionalization of titanium surfaces

Abstract

Mechanical properties and biological inertness of titanium provide potential in orthopedic and dental implants. However, integration of titanium-based implants into the existing tissue is a major problem. Herein, we demonstrate biofunctionalization of titanium surfaces through a mussel-inspired adhesion mechanism conjugated to self-assembled peptide nanofibers in order to overcome biocompatibility issues. A Dopa conjugated peptide nanofiber coating was used along with bioactive peptide sequences for osteogenic activity to enhance osseointegration of medical grade titanium surface, TiAl6V4 alloy. Dopa-mediated immobilization of osteogenic peptide nanofibers on titanium surfaces created an osteoconductive interface between osteoblast-like cells and inhibited adhesion and viability of soft tissue forming fibroblasts compared to the uncoated titanium substrate. This biofunctionalization strategy can be extended into other surface immobilization systems owing to the versatile adhesive properties of Dopa and the ease of ligand conjugation to peptide amphiphile molecules. © 2012 The Royal Society of Chemistry

A novel cytocompatible, hierarchical porous Ti6Al4V scaffold with immobilized silver nanoparticles

“…migration and differentiation) [17]. The MG63 cultured on the MAO/ Ag scaffolds observed by SEM after 5 d of incubation (Fig.…”

Biomaterial strategies for engineering implants for enhanced osseointegration and bone repair

“…BMP-2 is a fairly hydrophobic protein and adheres strongly to material surfaces and often denatures in the process losing its activity [78]. A sacrificial layer between implant and therapeutics has been proposed to overcome this issue [78–80]. Kashiwagi et al constructed an artificial protein containing 3 reversible material binding motifs for titanium and fused it with the N-terminal of BMP-2 [78].…”

Multifunctional biomaterial coatings: synthetic challenges and biological activity

“…In addition, differently charged peptide helices, which can build a coiled coil, can be used for a directed presentation of molecules for several biochemical applications (Reinhardt et al, 2014;Lequoy et al, 2016). An advantage of peptide self-assembly strategies is the generation of highly modular fibrils that can additionally create a structural ECM-mimetic to support cell adhesion (Collier, 2008;Jung et al, 2011;Ceylan et al, 2012). Functional molecules can be also combined by the high affinity between biotin and streptavidin (avidin) ( Figure 4C) (Freitas et al, 2012;Gorbahn et al, 2012).…”

“…Two different approaches are mainly used to apply mussel adhesive properties in the field of biomaterials. On the one hand, modular peptides containing DOPA can be synthesized and easily modified by solid phase peptides synthesis (SPPS) (Ceylan et al, 2012;Ham et al, 2013;Tang et al, 2014). On the other hand, DOPA is incorporated into polymers or recombinantly expressed proteins (mussel foot proteins from the blue mussel (Mytilus edulis) by enzymatic hydroxylation of tyrosine residues (Hwang et al, 2007).…”

“…Moreover, quinones result in a substantial loss of affinity to surfaces like titanium (Lee et al, 2006). Mussel derived peptides are easily modified with additional functionalities by SPPS or orthogonal click reactions, providing the possibility of exactly defining the chemical composition (Ceylan et al, 2012;Ham et al, 2013). The mussel adhesive approach was used to anchor cell-attracting as well as protein-repellent effects Wei et al, 2014;Kakinoki and Yamaoka, 2015).…”