Surface air plasma-induced cell death and cytokine release of human keratinocytes in the context of psoriasis

Zhong, Shi-yu; Dong, Yingying; Liu, D.X.; Xu, Dehui; Xiao, Shengxiang; Chen, Hailan; Kong, Michael G.

doi:10.1111/bjd.14236

Cited by 73 publications

(55 citation statements)

References 43 publications

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“…While several reports demonstrated that argon NTP promotes cell migration and proliferation, NTP generated from air induced cell death, due to the apoptosis or necroptosis related mechanisms2627. Despite the dramatically different effect in vitro , the enhancing plasma effect on wound healing was demonstrated in both argon161718 and air plasma22.…”

Section: Discussionmentioning

confidence: 99%

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Non-thermal air plasma promotes the healing of acute skin wounds in rats

Kubinová

Zaviskova

Uherkova

et al. 2017

Sci Rep

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Non-thermal plasma (NTP) has nonspecific antibacterial effects, and can be applied as an effective tool for the treatment of chronic wounds and other skin pathologies. In this study we analysed the effect of NTP on the healing of the full-thickness acute skin wound model in rats. We utilised a single jet NTP system generating atmospheric pressure air plasma, with ion volume density 5 · 1017 m−3 and gas temperature 30–35 °C. The skin wounds were exposed to three daily plasma treatments for 1 or 2 minutes and were evaluated 3, 7 and 14 days after the wounding by histological and gene expression analysis. NTP treatment significantly enhanced epithelization and wound contraction on day 7 when compared to the untreated wounds. Macrophage infiltration into the wound area was not affected by the NTP treatment. Gene expression analysis did not indicate an increased inflammatory reaction or a disruption of the wound healing process; transient enhancement of inflammatory marker upregulation was found after NTP treatment on day 7. In summary, NTP treatment had improved the healing efficacy of acute skin wounds without noticeable side effects and concomitant activation of pro-inflammatory signalling. The obtained results highlight the favourability of plasma applications for wound therapy in clinics.

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Section: Discussionmentioning

confidence: 99%

“…Remarkably, the biological effects of NTPs have been shown to be dose-dependent, ranging from stimulation of cell proliferation and migration1124 to cell death by necrosis25 or apoptosis1226. In this respect, using different plasma sources, operation parameters and other factors (working gas, plasma density, temperature, electric fields, ozone, UV, etc.)…”

mentioning

confidence: 99%

Non-thermal air plasma promotes the healing of acute skin wounds in rats

Kubinová

Zaviskova

Uherkova

et al. 2017

Sci Rep

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“…In view of promising results on the induction of neo-angiogenetic and epithelial growths effects by CAP treatment, we hypothesize that for the repetitive treatment of wounds the increase in blood flow might become a sustainable positive effect on vascular autoregulation in impaired tissue. 24,54,55…”

Section: Discussionmentioning

confidence: 99%

Effect of direct cold atmospheric plasma (diCAP) on microcirculation of intact skin in a controlled mechanical environment

Borchardt¹,

Ernst

Helmke

et al. 2017

Microcirculation

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ObjectiveThe microcirculatory response of intact human skin to exposure with diCAP for different durations with a focus on the effect of implied mechanical pressure during plasma treatment was investigated.MethodsLocal relative hemoglobin, blood flow velocity, tissue oxygen saturation, and blood flow were monitored noninvasively for up to 1 hour in 1‐2 mm depth by optical techniques, as well as temperature, pH values, and moisture before and after skin stimulation. The experimental protocol (N = 10) was set up to differentiate between pressure‐ and plasma‐induced effects.ResultsSignificant increases in microcirculation were only observed after plasma stimulation but not after pressure stimulus alone. For a period of 1 h after stimulation, local relative hemoglobin was increased by 5.1% after 270 seconds diCAP treatment. Tissue oxygen saturation increased by up to 9.4%, whereas blood flow was doubled (+106%). Skin pH decreased by 0.3 after 180 seconds and 270 seconds diCAP treatment, whereas skin temperature and moisture were not affected.ConclusionsdiCAP treatment of intact skin notably enhances microcirculation for a therapeutically relevant period. This effect is specific to the plasma treatment and not an effect of the applied pressure. Prolonged treatment durations lead to more pronounced effects.

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“…So far, the exact molecular mechanisms of how NTP changes cellular functionality are not known. It is believed, that the main biological consequences of cell treatment with NTP are intracellular RNS and ROS appearance 10–12 . However, in literature, there is substantial disagreement on the molecular consequences of NTP cell treatment 13, 14 .…”

Section: Introductionmentioning

confidence: 99%

Chemically different non-thermal plasmas target distinct cell death pathways

Lunov

Zablotskii

Churpita

et al. 2017

Sci Rep

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A rigorous biochemical analysis of interactions between non-thermal plasmas (NTPs) and living cells has become an important research topic, due to recent developments in biomedical applications of non-thermal plasmas. Here, we decouple distinct cell death pathways targeted by chemically different NTPs. We show that helium NTP cells treatment, results in necrosome formation and necroptosis execution, whereas air NTP leads to mTOR activation and autophagy inhibition, that induces mTOR-related necrosis. On the contrary, ozone (abundant component of air NTP) treatment alone, exhibited the highest levels of reactive oxygen species production leading to CypD-related necrosis via the mitochondrial permeability transition. Our findings offer a novel insight into plasma-induced cellular responses, and reveal distinct cell death pathways triggered by NTPs.

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Surface air plasma-induced cell death and cytokine release of human keratinocytes in the context of psoriasis

Cited by 73 publications

References 43 publications

Non-thermal air plasma promotes the healing of acute skin wounds in rats

Non-thermal air plasma promotes the healing of acute skin wounds in rats

Effect of direct cold atmospheric plasma (diCAP) on microcirculation of intact skin in a controlled mechanical environment

Chemically different non-thermal plasmas target distinct cell death pathways

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