Surface and Structure of Phosphatidylcholine Membranes Reconstructed with CoFe<sub>2</sub>O<sub>4</sub> Nanoparticles

Аносов, А. А.; Astanina, P.; Proskuryakov, I. I.; Коплак, О. В.; Моргунов, Р. Б.

doi:10.1021/acs.langmuir.2c02659

Cited by 4 publications

(4 citation statements)

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“…In [ 35 ], an increase in BLM permeability in the presence of nanoparticles was shown. Here, we decided to determine whether the current response of the membrane to triangular voltage and the properties of the membrane change when particles are added.…”

Section: Resultsmentioning

confidence: 99%

Bilayer Lipid Membrane as Memcapacitance: Capacitance–Voltage Pinched Hysteresis and Negative Insertion Conductance

Smirnova

Аносов

2023

Membranes

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Inelastic (dissipative) effects of different natures in lipid bilayer membranes can lead to hysteresis phenomena. Early, it was shown that lipid bilayer membranes, under the action of a periodic sinusoidal voltage, demonstrate pinched-hysteresis loops in the experimental capacitance–voltage dependences and are almost the only example of the physical implementation of memcapacitance. Here, we propose an equivalent circuit and mathematical framework for analyzing the dynamic nonlinear current response of a lipid bilayer membrane as an externally controlled memcapacitance. Solving a nonlinear differential equation for the equivalent circuit of a membrane in the form of a parallel connection of a nonlinear viscoelastic capacitor and an active resistance using the small parameter method, we obtain explicit analytical dependences for the current response of the membrane and pinched-hysteresis loops. The explicit solutions and their comparison with experimental data allow us to identify the lumped equivalent circuit parameters that govern the memcapacitor behavior of the membrane and hence the magnitude of the hysteresis. We quantify the memcapacitance hysteresis in terms of negative work done by the control signal. An analysis of the formulas leads to the conclusion that the determining factor for the appearance of pinched hysteresis is the type of nonlinear dependence of the device capacitance on voltage.

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Section: Resultsmentioning

confidence: 99%

Bilayer Lipid Membrane as Memcapacitance: Capacitance–Voltage Pinched Hysteresis and Negative Insertion Conductance

Smirnova

Аносов

2023

Membranes

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“…This interaction of cyt c with the bilayer is similar to the interaction of hydrophobic nanoparticles with the membrane. It was shown in [ 43 ] that cobalt ferrite nanoparticles form the through pores in asolectin and diphytanoylphosphatidylcholine membranes.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cytochrome C on the Conductance of Asolectin Membranes and the Occurrence of Through Pores at Different pHs

et al. 2023

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The study of the electrical parameters of asolectin bilayer lipid membranes in the presence of cytochrome c (cyt c) at various concentrations showed that an increase in the concentration of cyt c leads to an increase in the membrane conductance and the appearance of through pores. The studied membranes did not contain cardiolipin, which is commonly used in studying the effect of cyt c on membrane permeability. In the presence of cyt c, discrete current fluctuations were recorded. The occurrence of these fluctuations may be associated with the formation of through pores. The diameter of these pores was ~0.8 nm, which is smaller than the size of the cyt c globule (~3 nm). Measurements carried out at pH values from 6.4 to 8.4 showed that the concentration dependence of the membrane conductance increases with increasing pH. To assess the binding of cyt c to the bilayer, we measured the concentration and pH dependences of the difference in surface potentials induced by the unilateral addition of cyt c. The amount of bound cyt c at the same concentrations decreased with increasing pH, which did not correspond to the conductance trend. An analysis of conductance traces leads to the conclusion that an increase in the integral conductance of membranes is associated with an increase in the lifetime of pores. The formation of “long-lived” pores, of which the residence time in the open state is longer than in the closed state, was achieved at various combinations of pHs and cyt c concentrations: the higher the pH, the lower the concentration at which the long-lived pores appeared and, accordingly, a higher conductance was observed. The increase in conductance and the formation of transmembrane pores are not due to the electrostatic interaction between cyt c and the membrane. We hypothesize that an increase in pH leads to a weakening of hydrogen bonds between lipid heads, which allows cyt c molecules to penetrate into the membrane. This disrupts the order of the bilayer and leads to the occurrence of through pores.

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“…Изменения в структуре мембраны, вызванные магнитными наночастицами, могут приводить к увеличению ее проницаемости за счет образования мембранных пор [15,28]…”

Section: Discussionunclassified

Magnetite nanoparticles increase the conductivity of azolectin bilayer in an inhomogeneous magnetic field

Anosov,

Borisova,

Taranov

et al. 2023

JRE

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In the magnetofection method, magnetic fields and magnetic nanoparticles are used to increase the efficiency of gene delivery into cells. Magnefection enhances the introduction into cells of gene vectors with which magnetic nanoparticles are associated, due to the action of a magnetic field that holds the nanoparticles in the area of their application. It is believed that the magnetic field itself does not change the mechanism of absorption (endocytosis) of nanoparticles. Both the beneficial effect of magnetofection - delivery of the vector into the cell, and its side effect - cytotoxicity are associated with the interaction of particles with cell membranes and, in particular, with lipid bilayers. In our work, we investigated the effect of an applied stationary inhomogeneous magnetic field and spherical superparamagnetic magnetite nanoparticles with a diameter of about 4 nm on the conductivity of azolectin bilayer lipid membranes. The membranes were formed in a stationary magnetic field with a magnetic induction of up to 26 mT. The magnetic field had no effect on the conductivity of the membrane. After monitoring the membrane conductivity, magnetic nanoparticles were added to the solution surrounding the membrane. The addition was carried out on one side of the membrane so that the magnetic field attracted nanoparticles to the membrane surface. After adding nanoparticles in a magnetic field, the conductivity of the membranes increased by one to two orders of magnitude. This effect was observed for all membranes. A smooth increase in conductivity was accompanied in a number of cases (for 25% of the membranes) by the appearance of current jumps, which can be associated with the formation of through conducting pores with a radius of about 0.5 nm. The conductivity increased with increasing magnetic field gradient.

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Surface and Structure of Phosphatidylcholine Membranes Reconstructed with CoFe₂O₄ Nanoparticles

Cited by 4 publications

References 50 publications

Bilayer Lipid Membrane as Memcapacitance: Capacitance–Voltage Pinched Hysteresis and Negative Insertion Conductance

Bilayer Lipid Membrane as Memcapacitance: Capacitance–Voltage Pinched Hysteresis and Negative Insertion Conductance

Effect of Cytochrome C on the Conductance of Asolectin Membranes and the Occurrence of Through Pores at Different pHs

Magnetite nanoparticles increase the conductivity of azolectin bilayer in an inhomogeneous magnetic field

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Surface and Structure of Phosphatidylcholine Membranes Reconstructed with CoFe2O4 Nanoparticles

Cited by 4 publications

References 50 publications

Bilayer Lipid Membrane as Memcapacitance: Capacitance–Voltage Pinched Hysteresis and Negative Insertion Conductance

Bilayer Lipid Membrane as Memcapacitance: Capacitance–Voltage Pinched Hysteresis and Negative Insertion Conductance

Effect of Cytochrome C on the Conductance of Asolectin Membranes and the Occurrence of Through Pores at Different pHs

Magnetite nanoparticles increase the conductivity of azolectin bilayer in an inhomogeneous magnetic field

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Surface and Structure of Phosphatidylcholine Membranes Reconstructed with CoFe₂O₄ Nanoparticles