Surface Antigen Profiling of <i>Helicobacter pylori</i>‐Infected and ‐Uninfected Gastric Cancer Cells Using Antibody Microarray

Sukri, Asif; Hanafiah, Alfizah; Kosai, Nik Ritza; Taher, Mustafa Mohammed; Rose, Isa Mohamed

doi:10.1111/hel.12295

Cited by 10 publications

(22 citation statements)

References 53 publications

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“…Capture of single cell suspension of gastric epithelial cells onto DotScan TM slides has been described in previous study (Sukri et al 2016). The slides consist of more than 140 CD antibodies against CD antigens that have been customized to consist 82 established CD antibodies against T-, B-, and myeloid cells and another 42 CD antibodies were selected markers of gastric cancer cells and Helicobacter pylori infection (MedSaic Pty.…”

Section: Capturing Single Cell Suspension Of Gastric Epithelial Cellsmentioning

confidence: 99%

“…DotScan TM array employs more than 140 clusters of differentiation (CD) antibodies on one slide for profiling of many CD antigens in a single assay. Current DotScan TM antibody microarray consists of 82 CD antibodies against established CD antigens (T and B cells markers and myeloid cell markers) and 40 CD antibodies selected against specific disease that can be customized (Sukri et al 2016). CD antigens are cell surface molecules expressed mainly on leukocytes and other cells that are important in immune responses (Zhou et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…CD antigens are cell surface molecules expressed mainly on leukocytes and other cells that are important in immune responses (Zhou et al 2010). Hence, DotScan TM antibody microarray has been used to immunophenotype CD antigens in a number of diseases such as leukaemia (Belov et al 2003), colorectal cancer (Zhou et al 2010), liver diseases (Rahman et al 2012) and gastric cancer (Sukri et al 2016). DotScan TM antibody microarray enables rapid characterization of discrete leukemia subtypes whose CD antigens' expression may be correlated with leukemia typing.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Cryopreserved Single Cell Suspension of Gastric Epithelial Cells for Successful DotscanTM Antibody Microarray

Sukri

Hanafiah²,

Mahmood³

et al. 2019

JSM

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Cell cryopreservation plays a pivotal role for successful immunological assays. DotScan TM antibody microarray employs live cells with satisfactory cell viability for detection of clusters of differentiation (CD) antigens in various diseases. Effective cryopreservation contributes to successful capture of cells on DotScan TM antibody microarray. The purpose of this study was to evaluate the effect of cells viability on cells binding to specific CD antigens. Cells with similar concentration but different percentage of cell viabilities were captured on DotScan TM slides. Cells stored for 5 days at-80ºC and added with freezing medium in stepwise manner had higher viabilities as compared to cells stored for 6 months at-80ºC and added with freezing medium in direct manner (p=0.012). Cells with 88% viability had higher binding to CD44/CD29 control antibodies as compared to cells with 33% viability. Short-term storage of cells and the technique for freezing medium addition to cells play a critical role for successful cryopreservation of single cell suspension.

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Section: Capturing Single Cell Suspension Of Gastric Epithelial Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimization of Cryopreserved Single Cell Suspension of Gastric Epithelial Cells for Successful DotscanTM Antibody Microarray

Sukri

Hanafiah²,

Mahmood³

et al. 2019

JSM

Self Cite

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“…Helicobacter pylori is a pathogen that colonizes roughly one half of the world’s population which causes chronic gastritis. Sukri et al [ 40 ] employed the DotScan™ antibody microarray to determine the tolerance of immune system toward tumor cells in gastric cancer using 144 CD antibodies to profile the distribution of CD markers between Helicobacter pylori infected and un-infected gastric adenocarcinoma cells. Interestingly, they found gastric adenocarcinoma cell line AGS infected by cagA + H. pylori showed increased CD27 expression, which is essential for maintenance of T cell population, and increased CD markers were also detected in H. pylori -infected gastric cancer patients.…”

Section: Recent Applications (Since 2011)mentioning

confidence: 99%

Current applications of antibody microarrays

et al. 2018

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The concept of antibody microarrays is one of the most versatile approaches within multiplexed immunoassay technologies. These types of arrays have increasingly become an attractive tool for the exploratory detection and study of protein abundance, function, pathways, and potential drug targets. Due to the properties of the antibody microarrays and their potential use in basic research and clinical analytics, various types of antibody microarrays have already been developed. In spite of the growing number of studies utilizing this technique, few reviews about antibody microarray technology have been presented to reflect the quality and future uses of the generated data. In this review, we provide a summary of the recent applications of antibody microarray techniques in basic biology and clinical studies, providing insights into the current trends and future of protein analysis.Electronic supplementary materialThe online version of this article (10.1186/s12014-018-9184-2) contains supplementary material, which is available to authorized users.

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“…Based on these studies, an antibody microarray was successfully applied for the quality control of mesenchymal stem cells . Other research groups also reported the potential use of similar antibody microarrays in immunophenotyping. − …”

Section: Introductionmentioning

confidence: 99%

Quantitative Cell Subset Analysis Using Antibody Microarrays

Ogasawara

Kuwabara

Kozai

et al. 2021

ACS Appl. Bio Mater.

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The expression patterns of surface antigens are associated with the differentiation status and functional characteristics of mammalian cells. To analyze the surface antigen expression pattern in a high-throughput manner, antibody microarrays have been developed by several groups, including ours. This analysis can be performed using cell-binding assays on microarrays; moreover, this approach has advantages over conventional flow cytometry (FCM). Unlike FCM, the microarray-based method cannot evaluate the concurrent expression of more than two surface antigens on a single cell, and therefore, it cannot be used for cell subset analysis. To overcome this drawback, we prepared an antibody microarray with spots presenting co-immobilized multiple antibodies together with spots presenting each antibody separately. The co-immobilized spots are expected to be reactive for every surface antigen specific to the co-immobilized antibodies. In addition, the concept of an algebra of sets is incorporated into the derivation of quantitative data regarding cell subsets. Here, taking cell subsets with respect to two surface antigens as the simplest example, antibody microarrays were prepared and initially subjected to validation studies to verify the accuracy of cell-binding assays. Quantitative subset analysis was performed using antibody microarrays prepared using the anti-CD13 and anti-CD49f antibodies. For model populations that consisted of discrete subsets, THP-1, HL-60, CCRF-CEM, and Ramos cell lines were used because they were found by FCM to have a singular phenotype, that is, CD13+CD49f+, CD13+CD49f–, CD13–CD49f+, and CD13–CD49f–, respectively. Five populations were prepared by mixing these cells at various ratios and analyzed for their subsets using microarrays. The results showed that the experimentally determined abundance ratios of the four model subsets were in good agreement with the predetermined abundance ratios, which provided the proof of principle for the new method in the quantitative subset analysis.

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Surface Antigen Profiling of Helicobacter pylori‐Infected and ‐Uninfected Gastric Cancer Cells Using Antibody Microarray

Abstract: This study suggests the tolerance of immune system toward tumor cells in gastric cancer and distinct mechanisms of immune responses exploited by different H. pylori strains.

Cited by 10 publications

References 53 publications

Optimization of Cryopreserved Single Cell Suspension of Gastric Epithelial Cells for Successful DotscanTM Antibody Microarray

Optimization of Cryopreserved Single Cell Suspension of Gastric Epithelial Cells for Successful DotscanTM Antibody Microarray

Current applications of antibody microarrays

Quantitative Cell Subset Analysis Using Antibody Microarrays

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