Surface biomimetic modification with laminin-loaded heparin/poly-l-lysine nanoparticles for improving the biocompatibility

Liu, Tao; Hu, Youdong; Tan, Jianying; Liu, Shihui; Chen, Junying; Guo, Xin; Pan, Changjiang; Li, Xia

doi:10.1016/j.msec.2016.11.010

Cited by 28 publications

(14 citation statements)

References 33 publications

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“…Furthermore, heparin inhibited the platelet aggregation, thereby improving the blood compatibility of Mg alloy [93]. Chitosan and heparin also benefit the adhesion and proliferation of endothelial cells [94,95]. The approach of this study may open up a new window of modifying the surface of Mgbased implants, such as intravascular stents.…”

Section: Reviewmentioning

confidence: 87%

Advances in layer-by-layer self-assembled coatings upon biodegradable magnesium alloys

Shao

et al. 2021

Sci. China Mater.

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Magnesium (Mg) and its alloys are considered as ideal biodegradable materials due to their excellent mechanical properties and biocompatibility. In order to improve the surface properties to allow better adaptation to the surrounding tissue of the body, surface modification has played a significant role in satisfying multiple clinical requirements such as corrosion resistance, biocompatibility, and antibacterial ability. Here, layer-by-layer (LbL) self-assembly, which can be applied for biodegradable Mg alloys due to its extensive choice of usable units, holds great promise among all the surface techniques. In this review, the mechanisms of the driving force (i.e., electrostatic interaction, hydrogen bonding, charge transfer interaction and covalent bonding), cuttingedge advances in preparation methods (e.g., dipping, spraying, and spinning) and the functional properties (corrosion resistance, antibacterial activity, and biocompatibility) that could be achieved by the LbL coatings are summarized. A reasonable trend of the potential development of LbL for bio-Mg alloys is also proposed at the end of this article.

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Section: Reviewmentioning

confidence: 87%

Advances in layer-by-layer self-assembled coatings upon biodegradable magnesium alloys

Shao

et al. 2021

Sci. China Mater.

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“…The density and amount of the exposed heparin on the VEGF‐loaded nanoparticle immobilized surfaces were quantified by TBO assay. The detailed method is described in our previous work (Liu et al, ). The DM was used as a blank control.…”

Section: Methodsmentioning

confidence: 99%

Heparin/poly‐l‐lysine nanoplatform with growth factor delivery for surface modification of cardiovascular stents: The influence of vascular endothelial growth factor loading

Tan

Cui

Zeng

et al. 2020

J Biomedical Materials Res

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The rapid re‐endothelialization of the vascular stent surface is desirable for preventing thrombosis or reducing restenosis. Many biological factors that promote the biological behavior of endothelial cells have been used for the surface modification of stents. Vascular endothelial growth factor (VEGF), which plays an important role in angiogenesis, induces strong vascular growth. In this study, we investigated different VEGF concentrations (50 to 500 ng/ml) to determine the optimum concentration for biocompatibility. First, VEGF‐loaded heparin/poly‐l‐lysine (Hep‐PLL) nanoparticles were created by electrostatic interactions. Then, the VEGF‐loaded nanoparticles were immobilized on dopamine‐coated 316 L stainless steel (SS) surfaces. The physical and chemical properties of the modified surface were characterized and the biocompatibility was evaluated in vitro. The results indicated that the VEGF‐loaded nanoparticles were immobilized successfully on the 316LSS surface, as evidenced by the results of Alcian Blue staining and water contact angle (WCA) measurements. The low platelet adhesion and activation indicated that the modified surfaces had good blood compatibility. The modified surfaces showed a good inhibitory effect on smooth muscle cells, indicating that they inhibited tissue hyperplasia. In addition, the modified surfaces significantly promoted endothelial cell adhesion, proliferation, migration, and biological activity, especially VEGF concentration was 350 ng/ml (NPV350). The optical VEGF concentration of the surface modified Hep‐PLL nanoparticles was 350 ng/ml. The proposed method shows promise for potential applications for cardiovascular devices.

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“…Heparin is a “Star biomolecule” for its outstanding anti‐coagulant property which is widely applied for the surface modification of cardiovascular biomaterials by itself or coupled with other ECM proteins, such as collagen, peptide, and laminin, etc. On the application of heparin, immobilized density is a key parameter because excessive density and release of heparin may cause hemolysis which is a serious side‐effect for the implants intervention.…”

Section: In Situ Constructing Vascular Endothelial Growth Microenviromentioning

confidence: 99%

Engineering Cardiovascular Implant Surfaces to Create a Vascular Endothelial Growth Microenvironment

Zhang

Huang

2017

Biotechnology Journal

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Cardiovascular disease (CVD) is generally accepted as the leading cause of morbidity and mortality worldwide, and an increasing number of patients suffer from atherosclerosis and thrombosis annually. To treat these disorders and prolong the sufferers' life, several cardiovascular implants have been developed and applied clinically. Nevertheless, thrombosis and hyperplasia at the site of cardiovascular implants are recognized as long-term problems in the practice of interventional cardiology. Here, we start this review from the clinical requirement of the implants, such as anti-hyperplasia, anti-thrombosis, and pro-endothelialization, wherein particularly focus on the natural factors which influence functional endothelialization in situ, including the healthy smooth muscle cells (SMCs) environment, blood flow shear stress (BFSS), and the extracellular matrix (ECM) microenvironment. Then, the currently available strategies on surface modification of cardiovascular biomaterials to create vascular endothelial growth microenvironment are introduced as the main topic, e.g., BFSS effect simulation by surface micro-patterning, ECM rational construction and SMCs phenotype maintain. Finally, the prospects for extending use of the in situ construction of endothelial cells growth microenvironment are discussed and summarized in designing the next generation of vascular implants.

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Surface biomimetic modification with laminin-loaded heparin/poly-l-lysine nanoparticles for improving the biocompatibility

Cited by 28 publications

References 33 publications

Advances in layer-by-layer self-assembled coatings upon biodegradable magnesium alloys

Advances in layer-by-layer self-assembled coatings upon biodegradable magnesium alloys

Heparin/poly‐l‐lysine nanoplatform with growth factor delivery for surface modification of cardiovascular stents: The influence of vascular endothelial growth factor loading

Engineering Cardiovascular Implant Surfaces to Create a Vascular Endothelial Growth Microenvironment

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