Surface characterization and chondrogenic differentiation of mesenchymal stromal cells derived from synovium

Jo, Chris Hyunchul; Ahn, Hyo‐Jeong; Kim, H.J.; Seong, Sang Cheol; Lee, M.C.

doi:10.1080/14653240701291620

Cited by 64 publications

(53 citation statements)

References 46 publications

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“…Most cell-surface markers in MSCs were stable after the first passage, as has been described by other groups. 47 In agreement with our results, Jo et al 47 observed that CD14, CD34, and HLA-DR disappeared after the first passage. Diaz-Romero et al 48 found that surface markers on MSCs showed little variation during the culture.…”

Section: Phenotype Of Mesenchymal Cellssupporting

confidence: 82%

Phenotype and chondrogenic differentiation of mesenchymal cells from adipose tissue of different species

Martínez‐Lorenzo

Royo-Cañas

Alegre-Aguarón

et al. 2009

Journal Orthopaedic Research

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Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into several mesoderm lineages. They have been isolated from different tissues, such as bone marrow, adult peripheral blood, umbilical cord blood, and adipose tissue. The aim of this study was to analyze the differences in proliferation and phenotype of adipose tissue-derived MSCs from three different species, and to evaluate their capacity to differentiate into chondrocytes in vitro. A comparative study of cultured human, rabbit, and sheep mesenchymal cells from adipose tissue was carried out, and the main morphological parameters, proliferative activity, and expression of surface markers were characterized. Proliferation and flow cytometry data showed species-related differences between animal and human MSCs. Histological staining suggested that rabbit and sheep mesenchymal cells were able to differentiate into chondrocytic lineages. Human mesenchymal cells, though they could also differentiate, accomplished it with more difficulty than animal MSCs. These results could help to explain the differences in the chondrogenic capacity of sheep and rabbit MSCs when they are used as animal models compared to human mesenchymal cells in a clinical assay. ß

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Section: Phenotype Of Mesenchymal Cellssupporting

confidence: 82%

Phenotype and chondrogenic differentiation of mesenchymal cells from adipose tissue of different species

Martínez‐Lorenzo

Royo-Cañas

Alegre-Aguarón

et al. 2009

Journal Orthopaedic Research

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“…We cannot exclude the possibility that CD105 would identify MSCs in humans but not in mice, because variations in MSC phenotype and biology across species are known (34). In addition, acquisition of CD105 might occur as a result of phenotypic rearrangements during ex vivo cell manipulations (35). Indeed, markers observed on cells in vitro cannot be directly extrapolated to the equivalent native cells in vivo.…”

Section: Discussionmentioning

confidence: 99%

Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo

Kurth¹,

Dell’Accio²,

Crouch³

et al. 2011

Arthritis & Rheumatism

180

174

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Objective. We previously reported that human synovium contains cells that, after culture expansion, display properties of mesenchymal stem cells (MSCs). The objective of this study was to identify MSCs in native synovium in vivo.Methods. To identify stem cells in the synovium in vivo, a double nucleoside analog cell-labeling scheme was used in a mouse model of joint-surface injury. For labeling of slow-cycling cells, mice received iododeoxyuridine (IdU) for 30 days, followed by a 40-day washout period. For labeling of cells that proliferate after injury, mice underwent knee surgery to produce an articular cartilage defect and received chlorodeoxyuridine (CIdU) for 4 days, starting at multiple time points after surgery. Unoperated and sham-operated joints served as controls. Knee joint paraffin sections were analyzed by double and triple immunostaining to detect nucleoside analogs, conventional MSC markers, and chondrocytelineage markers.Results. Long-term-retaining, slow-cycling IdUpositive cells were detected in the synovium. At 4 days and 8 days after injury, there was marked proliferation of IdU-positive cells, which costained for CIdU. IdUpositive cells were nonhematopoietic, nonendothelial stromal cells, were distinct from pericytes, and stained positive for MSC markers. MSCs were phenotypically heterogeneous and located in topographically distinct niches in the lining layer and the subsynovial tissue. Twelve days after injury, double nucleoside-labeled cells within synovium were embedded in cartilagespecific metachromatic extracellular matrix and costained positive for the chondrocyte-lineage markers Sox9 and type II collagen.Conclusion. Our findings provide the first evidence of the existence of resident MSCs in the knee joint synovium that undergo proliferation and chondrogenic differentiation following injury in vivo.

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“…5 We plated cells at a low density and allowed progenitor cells to occupy a larger proportion of the cell population. 38 Synovium-derived cells maintained their proliferative capacities over at least eight passages spanning 130 days. The average number of cumulative PDs in 130 days was 43 (range 36-47), much larger than that of BM-MSCs (30, range 26-32).…”

Section: Discussionmentioning

confidence: 99%

“…The most dramatic changes in surface molecule expressions occur after the first passage, and in particular, that CD73, CD105, and CD166, which are known to be fairly specific for MSCs, either showed increased expression or newly appeared. 38 Studies performed passages using high seeding densities (1:4 dilution or 5 Â 10 4 cells/100 mm-dish) showed that total expansion rates were low, so only the early stage phenotype was achieved during in vitro expansion. Our study demonstrates that synovium-derived cells have a relatively long-term self-renewing ability with no abnormal karyotype changes.…”

Section: Discussionmentioning

confidence: 99%

Changes in chondrogenic phenotype and gene expression profiles associated with the in vitro expansion of human synovium‐derived cells

Han

Lee

Kim

et al. 2010

Journal Orthopaedic Research

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We undertook this study to characterize changes in the proliferative capacities, chondrogenic phenotypes, and gene expression profiles of human synovium-derived progenitor cells from osteoarthritic patients during in vitro expansion. Cells isolated from osteoarthritic synovia were cultured, and growth rates during serial passages were evaluated. Surface molecule expressions were determined by flow cytometry and cytogenetic analyses were performed. After chondrogenic differentiation in cell pellets, we evaluated type II collagen and glycosaminoglycan (GAG) synthesis. To assess whether the in vitro expansion of synovium-derived cells affects gene expression, we performed microarray analyses on cells at passage 0, 1, 2, 4, 6, and 8. Synovium-derived cells were rapidly expanded in vitro through passage 8 (about 130 days), and after passage 6, the proliferation rates decreased slightly with a wide range of individual variations. The expressions of CD166, CD49a, and CD106 decreased, whereas those of CD10, CD29, CD44, CD73, CD90, and CD105 showed no significant change. Karyotype analysis revealed no evidence of chromosome abnormalities. The staining of type II collagen and GAG in differentiated cell pellets showed rapid weakening. Genome-wide microarray analysis showed that synovium-derived cells from late passages over-expressed genes associated with cell cycle prolongation and cell aging, and less-expressed genes associated with cell growth stimulation. The in vitro expansion of synovium-derived cells was accompanied with decreased proliferative capacity and the chondrogenic phenotype, which might be modulated by change in gene expression patterns. ß

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Surface characterization and chondrogenic differentiation of mesenchymal stromal cells derived from synovium

Cited by 64 publications

References 46 publications

Phenotype and chondrogenic differentiation of mesenchymal cells from adipose tissue of different species

Phenotype and chondrogenic differentiation of mesenchymal cells from adipose tissue of different species

Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo

Changes in chondrogenic phenotype and gene expression profiles associated with the in vitro expansion of human synovium‐derived cells

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