Surface Characterization Using Atomic Force Microscopy (AFM) in Liquid Environments

Lyles, Venetia D.; Serem, Wilson K.; Yu, Jing‐Jiang; Garno, Jayne C.

doi:10.1007/978-3-642-34243-1_20

Cited by 10 publications

(4 citation statements)

References 101 publications

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“…Liquid environments expand the capabilities for scanning probe protocols to provide insight for dynamic processes at the nanoscale [ 29 ]. For example, studies of the elastic modulus of SAMS and protein films was accomplished in liquid media using force modulation AFM [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Surface assembly and nanofabrication of 1,1,1-tris(mercaptomethyl)heptadecane on Au(111) studied with time-lapse atomic force microscopy

Tian¹,

Singhana²,

Englade-Franklin³

et al. 2014

Beilstein J. Nanotechnol.

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SummaryThe solution self-assembly of multidentate organothiols onto Au(111) was studied in situ using scanning probe nanolithography and time-lapse atomic force microscopy (AFM). Self-assembled monolayers (SAMs) prepared from dilute solutions of multidentate thiols were found to assemble slowly, requiring more than six hours to generate films. A clean gold substrate was first imaged in ethanolic media using liquid AFM. Next, a 0.01 mM solution of multidentate thiol was injected into the liquid cell. As time progressed, molecular-level details of the surface changes at different time intervals were captured by successive AFM images. Scanning probe based nanofabrication was accomplished using protocols of nanografting and nanoshaving with n-alkanethiols and a tridentate molecule, 1,1,1-tris(mercaptomethyl)heptadecane (TMMH). Nanografted patterns of TMMH could be inscribed within n-alkanethiol SAMs; however, the molecular packing of the nanopatterns was less homogeneous compared to nanopatterns produced with monothiolates. The multidentate molecules have a more complex assembly pathway than monothiol counterparts, mediated by sequential steps of forming S–Au bonds to the substrate.

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Section: Resultsmentioning

confidence: 99%

Surface assembly and nanofabrication of 1,1,1-tris(mercaptomethyl)heptadecane on Au(111) studied with time-lapse atomic force microscopy

Tian¹,

Singhana²,

Englade-Franklin³

et al. 2014

Beilstein J. Nanotechnol.

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show abstract

“…3D surface topography was provided by Atomic Force Microscopy (AFM), the measures of which relying on van der Waals or other attractive and repulsive forces [26]. Five drops of chitosan-alginate nanoparticle were added on a glass slide and left until drying and precipitating.…”

Section: Characterization Of the Prepared Nanoparticlesmentioning

confidence: 99%

The synergistic effect of chitosan-alginate nanoparticle loaded with doxycycline antibiotic against multidrug resist enterobacteriaceae

alsaady

2020

eijs

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This study focused on the synthesis of chitosan-alginate (CH-ALg) nanoparticles by ionotropic gelation technique using sodium alginate and calcium chloride. The particle size of the synthesized nanoparticles was confirmed by atomic force microscope (AFM) and it was 61.9 nm. While the nature of functional groups present in chitosan nanoparticles was determined by FT-IR analysis. The antibacterial activity of chitosan-alginate was tested against multidrug resistance (MDR) gram- positive (Enterococcus faecalis) and gram-negative (Proteus mirabilis) bacteria. The results showed a significant effect against MDR isolates. The nanoparticles were loaded with the antibiotic doxycycline in order to improve the antibacterial activity and drug delivery efficiency. The synergistic effects of the biosynthesized chitosan-alginate and the loaded doxycycline at different concentrations against MDR bacteria were also investigated. The results showed that doxycycline-loaded nanoparticles have superior effectiveness compared to native doxycycline against gram negative and gram positive bacteria.

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“…Atomic Force Microscopy (AFM) offers 3-dimensional surface topography that is dependent on Van der Waals or even other attractive and repulsive forces [24]. In the glass slide, 5 drops of CH-Alg nanoparticle have been applied and left to dry and precipitate upon this.…”

Section: Characterization Of Prepared Nanoparticlementioning

confidence: 99%

Evaluation of Chitosan-Alginate Nanoparticle as A Stable Antibacterial Formula in Biological Fluids

Kadhum

Al-Ogaidi

2022

eijs

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This research mainly focuses on the preparation of chitosan-alginate Nanoparticle by ionotropic gelation method using calcium chloride and sodium alginate to form nanocomposites of CH-ALg, examine their antibacterial activity against multidrug resistance (MDR) bacteria, and evaluate the stability of chitosan-alginate formula in different biological fluids, including simulated gastric fluid (SGF) and intestinal fluid (SIF). The average diameter of particles size prepared was measured by an Atomic force microscope (AFM) and it was 61.91 nm. Otherwise, the nature of functional groups present in CH-ALg nanoparticle was investigated by Fourier transforms infrared (FTIR) analysis. The stability of synthesized CH-ALg nanoparticle was measured by Zeta potential measurement and show high stability with a 79 mV ratio. The SEM picture shows that particles are shown to be in the form of bundles and the size of nanoparticles was in the range of (14-84nm). The antibacterial activity of CH-ALg was tested against (MDR) Gram-positive bacteria (staphylococcus aureus) and Gram-negative bacteria (Salmonella typhi, Pseudomonas aeruginosa, and Enterobacter cloacae). The results exhibited a significant impact of antibacterial action against isolates of MDR. CH-ALg nanoparticles were loaded with antibiotic doxycycline in order to strengthen the antibacterial action and drug delivery effectiveness. The synergistic effects of prepared CH-ALg loaded with DOX antibiotic at several concentrations toward MDR bacteria were also examined. The results indicate that DOX-loaded nanoparticles have a significant improvement for antibacterial activity against Gram-negative and Gram-positive compared to doxycycline alone. The formula of chitosan- alginate was stabile in various biological fluids including simulated gastric fluid (SGF) and simulated intestinal fluid (SiF)

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Surface Characterization Using Atomic Force Microscopy (AFM) in Liquid Environments

Cited by 10 publications

References 101 publications

Surface assembly and nanofabrication of 1,1,1-tris(mercaptomethyl)heptadecane on Au(111) studied with time-lapse atomic force microscopy

Surface assembly and nanofabrication of 1,1,1-tris(mercaptomethyl)heptadecane on Au(111) studied with time-lapse atomic force microscopy

The synergistic effect of chitosan-alginate nanoparticle loaded with doxycycline antibiotic against multidrug resist enterobacteriaceae

Evaluation of Chitosan-Alginate Nanoparticle as A Stable Antibacterial Formula in Biological Fluids

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