Surface Chemistry and Cell Biological Tools for the Analysis of Cell Adhesion and Migration

Pulsipher, Abigail; Yousaf, Muhammad N.

doi:10.1002/cbic.200900787

Cited by 35 publications

(33 citation statements)

References 119 publications

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“…18, 27, 28 Despite the use of compliant hydrogels, these methods are often difficult to scale down, tune, and require significant quantities of protein or peptide, much of which does not bind. Here, we used density gradient multilayer polymerization (DGMP), to construct compliant culture substrates with spatial gradients of composition.…”

Section: Introductionmentioning

confidence: 99%

Haptotaxis is Cell Type Specific and Limited by Substrate Adhesiveness

Wen

Choi²,

Taylor‐Weiner

et al. 2015

Cel. Mol. Bioeng.

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Motile cells navigate through tissue by relying on tactile cues from gradients provided by extracellular matrix (ECM) such as ligand density or stiffness. Mesenchymal stem cells (MSCs) and fibroblasts encounter adhesive or ‘haptotactic’ gradients at the interface between healthy and fibrotic tissue as they migrate towards an injury site. Mimicking this phenomenon, we developed tunable RGD and collagen gradients in polyacrylamide hydrogels of physiologically relevant stiffness using density gradient multilayer polymerization (DGMP) to better understand how such ligand gradients regulate migratory behaviors. Independent of ligand composition and fiber deformation, haptotaxis was observed in mouse 3T3 fibroblasts. Human MSCs however, haptotaxed only when cell-substrate adhesion was indirectly reduced via addition of free soluble matrix ligand mimetic peptides. Under basal conditions, MSCs were more contractile than fibroblasts. However, the presence of soluble adhesive peptides reduced MSC-induced substrate deformations; increased contractility may contribute to limited migration, but modulating cytoskeletal assembly was ineffective at promoting MSC haptotaxis. When introduced to gradients of increased absolute ligand concentrations, 3T3s displayed increased contractility and no longer haptotaxed. These data suggest that haptotactic behaviors are limited by adhesion and that although both cell types may home to tissue to aid in repair, fibroblasts may be more responsive to ligand gradients than MSCs.

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Section: Introductionmentioning

confidence: 99%

Haptotaxis is Cell Type Specific and Limited by Substrate Adhesiveness

Wen

Choi²,

Taylor‐Weiner

et al. 2015

Cel. Mol. Bioeng.

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“…In addition, cell migration also plays a prominent role in many diseases such as vascular disease, tumour cell metastasis and chronic inflammation 1,2 . While the classical states of cell migration -polarization, protrusion extension, formation of adhesion, force generation and rear retraction -are generally accepted 3,4 , the elucidation of spatiotemporal mechanisms by which signal integration is coordinated has been more challenging.…”

Section: Introductionmentioning

confidence: 99%

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Ngalim

Magenau

Zhu

et al. 2013

JoVE

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Cells can sense and migrate towards higher concentrations of adhesive cues such as the glycoproteins of the extracellular matrix and soluble cues such as growth factors. Here, we outline a method to create opposing gradients of adhesive and soluble cues in a microfluidic chamber, which is compatible with live cell imaging. A copolymer of poly-L-lysine and polyethylene glycol (PLL-PEG) is employed to passivate glass coverslips and prevent non-specific adsorption of biomolecules and cells. Next, microcontact printing or dip pen lithography are used to create tracks of streptavidin on the passivated surfaces to serve as anchoring points for the biotinylated peptide arginine-glycine-aspartic acid (RGD) as the adhesive cue. A microfluidic device is placed onto the modified surface and used to create the gradient of adhesive cues (100% RGD to 0% RGD) on the streptavidin tracks. Finally, the same microfluidic device is used to create a gradient of a chemoattractant such as fetal bovine serum (FBS), as the soluble cue in the opposite direction of the gradient of adhesive cues. Video LinkThe video component of this article can be found at

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“…So far, the resolution is limited by the size of both the tip and the meniscus forming between the tip and the substrate. For many biological applications the current resolution is adequate; in fact, DPN is a powerful research tool for manipulating cells at subcellular resolution 171 and changing the growth conditions for these cells. This resolution is, however, not sufficient for the future of Integrated Circuit (IC) manufacturing, but closely related lithography methods such as local anodic oxidation reach sub-10 nm resolution 172 proving that better resolution is possible with DPN when combined with an electrically driven reaction.…”

Section: Discussionmentioning

confidence: 99%