Surface chemistry influences cancer killing effect of TiO2 nanoparticles

Thevenot, Paul; Cho, Jai; Wavhal, Dattatray S.; Timmons, Richard B.; Tang, Liping

doi:10.1016/j.nano.2008.04.001

Cited by 209 publications

(125 citation statements)

References 57 publications

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“…[28][29][30] In addition to surface functionalization, other factors such as particle size, shape, surface charge, and surface functionality also affect the interactions between particles and cells. 31,32 In our study, iron oxide NP surface was modified with dextran as confirmed by FTIR analysis. Utilizing DIONPs, we observed that the size of NPs in culture media was greater than its primary size, which is most likely due to the formation of the protein corona on the NPs in the buffer medium.…”

Section: Discussionmentioning

confidence: 59%

Toxicological evaluation of dextran stabilized iron oxide nanoparticles in human peripheral blood lymphocytes

Easo

Mohanan

2016

Biointerphases

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Iron oxide nanoparticles present an attractive choice for carcinogenic cell destruction via hyperthermia treatment due to its small size and magnetic susceptibility. Dextran stabilized iron oxide nanoparticles (DIONPs) synthesized and characterized for this purpose were used to evaluate its effect on cellular uptake, cytotoxicity, and oxidative stress response in human peripheral blood lymphocytes. In the absence of efficient internalization and perceptible apoptosis, DIONPs were still capable of inducing significant levels of reactive oxygen species formation shortly after exposure. Although these particles did not cause any genotoxic effect, they enhanced the expression of a few relevant oxidative stress and antioxidant defense related genes, accompanied by an increase in the glutathione peroxidase activity. These results indicate that under the tested conditions, DIONPs induced only minimal levels of oxidative stress in lymphocytes. Understanding the biological interaction of DIONPs, the consequences as well as the associated mechanisms in vitro, together with information obtained from systemic studies, could be expected to advance the use of these particles for further clinical trials.

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Section: Discussionmentioning

confidence: 59%

Toxicological evaluation of dextran stabilized iron oxide nanoparticles in human peripheral blood lymphocytes

Easo

Mohanan

2016

Biointerphases

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“…68 Wu et al 93 have 94 It is known that surface functionality of nanomaterials directly affects cell viability and intracellular particle accumulation. 83,95 Both graphene and SWCNTs induce cytotoxic effects, which depend on their concentration and shape.…”

Section: Concentrations (µG/ml) Ros Generation (Fold Increase)mentioning

confidence: 99%

Reduction of graphene oxide by resveratrol: a novel and simple biological method for the synthesis of an effective anticancer nanotherapeutic molecule

Gurunathan¹,

Han²,

Kim³

et al. 2015

IJN

158

129

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Objective Graphene represents a monolayer or a few layers of sp 2 -bonded carbon atoms with a honeycomb lattice structure. Unique physical, chemical, and biological properties of graphene have attracted great interest in various fields including electronics, energy, material industry, and medicine, where it is used for tissue engineering and scaffolding, drug delivery, and as an antibacterial and anticancer agent. However, graphene cytotoxicity for ovarian cancer cells is still not fully investigated. The objective of this study was to synthesize graphene using a natural polyphenol compound resveratrol and to investigate its toxicity for ovarian cancer cells. Methods The successful reduction of graphene oxide (GO) to graphene was confirmed by UV-vis and Fourier transform infrared spectroscopy. Dynamic light scattering and scanning electron microscopy were employed to evaluate particle size and surface morphology of GO and resveratrol-reduced GO (RES-rGO). Raman spectroscopy was used to determine the removal of oxygen-containing functional groups from GO surface and to ensure the formation of graphene. We also performed a comprehensive analysis of GO and RES-rGO cytotoxicity by examining the morphology, viability, membrane integrity, activation of caspase-3, apoptosis, and alkaline phosphatase activity of ovarian cancer cells. Results The results also show that resveratrol effectively reduced GO to graphene and the properties of RES-rGO nanosheets were comparable to those of chemically reduced graphene. Biological experiments showed that GO and RES-rGO caused a dose-dependent membrane leakage and oxidative stress in cancer cells, and reduced their viability via apoptosis confirmed by the upregulation of apoptosis executioner caspase-3. Conclusion Our data demonstrate a single, simple green approach for the synthesis of highly water-dispersible functionalized graphene nanosheets, suggesting a possibility of replacing toxic hydrazine by a natural and safe phenolic compound resveratrol, which has similar efficacy in the reduction of GO to rGO. Resveratrol-based GO reduction would facilitate large-scale production of graphene-based materials for the emerging graphene-based technologies and biomedical applications.

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“…To date, most nanotoxicity research has been focused on individual nano-sized metal oxides. The potential toxicity of nanoparticles, including nickel oxide (NiO), titanium dioxide (TiO 2 ), carbon nanotubes, and fullerenes, has drawn significant attention [13][14][15][16]. For example, NiO particles have been shown to trigger reactive oxygen species (ROS) that cause DNA damage in lungs of rats [13].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells.

Ada

Türk²,

Oğuztüzün³

et al. 2011

Folia Histochem Cytobiol.

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Abstract:The aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 μg/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 μg/mL concentration for 16 h, but high in 400-500 μg/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 μg/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.

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Surface chemistry influences cancer killing effect of TiO2 nanoparticles

Cited by 209 publications

References 57 publications

Toxicological evaluation of dextran stabilized iron oxide nanoparticles in human peripheral blood lymphocytes

Toxicological evaluation of dextran stabilized iron oxide nanoparticles in human peripheral blood lymphocytes

Reduction of graphene oxide by resveratrol: a novel and simple biological method for the synthesis of an effective anticancer nanotherapeutic molecule

Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells.

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