Surface composition of insulin and albumin adsorbed on polymer substrates as revealed by multivariate analysis of ToF‐SIMS data

Henry, Marie; Bertrand, Patrick

doi:10.1002/sia.2993

Cited by 39 publications

(40 citation statements)

References 31 publications

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“…They were selected according to literature. [48,[54][55][56][57] Furthermore, the spectra of the linker-modified TiO 2 samples were compared with the spectra of the protein-coated samples, and only signals that did not show significant intensities on the linker samples were chosen for evaluation and added to obtain the 'sum of protein signals' ('sum protein', cf supporting information S2). Figure 3b shows the intensity of a selection of these 'non-conflicting' amino acid signals versus the sum of all signals identified as protein specific (and non-conflicting, positive polarity, the obtained information is independent of coverage).…”

Section: Resultsmentioning

confidence: 99%

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Influence of bioactive linker molecules on protein adsorption

Killian

Schmuki

2014

Surface & Interface Analysis

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When protein coatings are used in surgical implants, for sensors or in food applications, it is important to be aware of the influence of the immobilization method onto the protein-coating performance. In this manuscript, we report on the effect of several bioactive linker molecules on a protein immobilized on a TiO 2 surface. Particularly, the protein adsorption, orientation and functionality, which are directly related to the tertiary structure of the molecule, were investigated.The protein horseradish peroxidase was immobilized on a TiO 2 substrate via a range of linker molecules for biomolecule attachment. The chosen linkers can immobilize the proteins in a random fashion via free NH 2 -groups or COOH-groups. Protein coverage was quantified with XPS, while Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provided information about protein orientation as well as changes in the tertiary structure in relation to the respective linker system.

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Section: Resultsmentioning

confidence: 99%

“…Signals were identified using the accurate mass as well as their isotopic pattern and assigned to amino acids according to references. [48,[54][55][56][57] Poisson correction was used for integration of the signal intensities. Spectra were normalized to their total intensity and calibrated on C …”

Section: Surface Analysismentioning

confidence: 99%

Influence of bioactive linker molecules on protein adsorption

Killian

Schmuki

2014

Surface & Interface Analysis

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“…Adsorbed protein films have been characterised (Lhoest et al, 2001;Wagner et al, 2002a;Wagner et al, 2002b;Wagner et al, 2003a;Wagner et al, 2003b;Bernsmann et al, 2008;Tyler et al, 2011) and even conformational changes (Xia et al, 2002) and denaturation of proteins (Killian et al, 2011) can be detected with ToF-SIMS. Surface characterisations of extracellular matrix scaffolds (Canavan et al, 2007, Brown et al, 2010Barnes et al, 2011) and insulin and albumin coated surfaces have been published (Henry and Bertrand, 2009). In the field of microbiology, ToF-SIMS has been applied to imaging colonies of microbes (Tyler et al, 2006, Esquenazi et al, 2009, to characterise intact, individual bacterial spores in 3D (Ghosal et al, 2008) and to detect antibiotics within intact bacterial colony biofilms (Gasper et al, 2008).…”

Section: Wwwecmjournalorg E Kaivosoja Et Al Spectroscopy In the Anmentioning

confidence: 99%

“…This limited peak set was constructed using unique amino acid fragmentation patterns of ToF-SIMS data previously identified for characterising proteins. Table 1 shows the selected peaks together with the amino acid they are characteristic for and the proportion of that amino acid (Samuel et al, 2001;Canavan et al, 2006;Henry and Bertrand, 2009). Peaks that can originate from several different amino acids (marked with a star in Table 1) were not included in the analysis.…”

Section: Principal Component Analysis (Pca)mentioning

confidence: 99%

Spectroscopy in the analysis of bacterial and eukaryotic cell footprints on implant surfaces

Kaivosoja¹,

Virtanen²,

Rautemaa³

et al. 2012

eCM

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We tested the suitability of two spectroscopic methods, x-ray photoelectron spectroscopy (XPS) and time of flight secondary ion mass spectrometry (ToF-SIMS), in the recognition of bacterial and eukaryotic cell footprints on implant surfaces. Human mesenchymal stem cells (MSCs) and Staphylococcus aureus were cultured on sample surfaces and detached using trypsin. Scanning electron microscopy confirmed that the processed surfaces did not contain any human or microbial cells. The footprints were then analysed using XPS and ToF-SIMS. XPS results showed no significant differences between the footprints, but principal component analysis of the ToF-SIMS data enabled clear separation of MSC-footprints from the S. aureus and co-culture footprints (p < 0.03). ToF-SIMS also demonstrated 'race for the surface' between proteins, which suggest surface charge (zeta-potential) dependent protein adsorption. ToF-SIMS differentiated eukaryotic and bacterial footprints and has potential for post-hoc detection of implant-related infections based on the typical ToF-SIMS spectra.

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“…Previous studies [26,36] reported that PVP, which impregnates the commercially available PC membranes, can somehow inhibit the adsorption of proteins on the polymer, even if such an adsorption is not completely prevented [37]. For this reason we performed some preliminary experiments comparing BSA and Cas adsorption on PVP coated vs. PVP-free PC membranes.…”

Section: Voltammetry At Protein Treated Neesmentioning

confidence: 99%

Modification of nanoelectrode ensembles by thiols and disulfides to prevent non specific adsorption of proteins

Silvestrini

Schiavuta²,

Scopece³

et al. 2011

Electrochimica Acta

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a b s t r a c tThe possibility to functionalize selectively with thiols or disulfides the surface of the gold nanoelectrodes of polycarbonate templated nanoelectrode ensembles (NEEs) is studied. It is shown that the Au nanoelectrodes can be coated by a self assembled monolayer (SAM) of thioctic acid (TA) or 2-mercaptoethanesulfonic (MES) acid. The study of the electrochemical behavior of SAM-modified NEEs by cyclic voltammetry (CV) at different solution pH, using ferrocenecarboxylate as an anionic redox probe (FcCOO − ) and (ferrocenylmethyl)trimethylammonium (FA + ) as a cationic redox probe, demonstrate that the SAM-modified nanoelectrodes are permselective, in that only cationic or neutral probes can access the SAM-coated nanoelectrode surface. CV, AFM and FTIR-ATR data indicate that proteins such as casein or bovine serum albumin, which are polyanionic at pH 7, adsorb on the surface of NEEs untreated with thiols, tending to block the electron transfer of the ferrocenyl redox probes. On the contrary, the pretreatment of the NEE with an anionic SAM protects the nanoelectrodes from protein fouling, allowing the detection of well shaped voltammetric patterns for the redox probe. Experimental results indicate that, in the case of MES treated NEEs, the protein is bound only onto the polycarbonate surface which surrounds the nanoelectrodes, while the tips of the gold nanoelectrodes remain protein free.

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Surface composition of insulin and albumin adsorbed on polymer substrates as revealed by multivariate analysis of ToF‐SIMS data

Cited by 39 publications

References 31 publications

Influence of bioactive linker molecules on protein adsorption

Influence of bioactive linker molecules on protein adsorption

Spectroscopy in the analysis of bacterial and eukaryotic cell footprints on implant surfaces

Modification of nanoelectrode ensembles by thiols and disulfides to prevent non specific adsorption of proteins

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