Surface-enhanced Raman scattering nanoprobes for the simultaneous detection of endogenous hypochlorous acid and peroxynitrite in living cells

Application of molecular SERS nanosensors: where we stand and where we are headed towards?

“…As most often these by-products are too small or are not Raman active in order to be directly detected by SERS, molecules that show chemical reactivity with ROS have to be assembled on the surface of the plasmonic NPs. For example hydrogen peroxide was detected using 4-mercaptophenylboronic ester (4-MPBE) [ 13 , 19 ], hypochlorous acid with 2-mercapto-4-methoxy-phenol (MMP) [ 20 , 21 ] and peroxynitrate with 4-mercaptophenylboronic acid pinacol ester (MBAPE) [ 21 , 22 ].…”

“…a Fabrication and sensing mechanism. b Simultaneous SERS imaging of the HOCl and ONOO − in living cells (reprinted from [ 21 ], Copyright 2018 with permission from Elsevier) …”

When the strategies for cellular selectivity fail. Challenges and surprises in the design and application of fluorescent benzothiadiazole derivatives for mitochondrial staining

“…37 For instance, reactive oxygen species (ROS) imbalances may be a consequence of specific mitochondrial labelling by a new fluorophore, but this issue is not addressed when a new mitochondrial marker is published, unless the bioprobe is developed to detect ROS, as described in several and recent examples. [38][39][40][41][42][43][44][45][46][47][48] We have already disclosed some mitochondrial markers such as fluorescent peptoids, 49 lapachone-based structures, 50 Goniothalamin derivative, 51 multicomponent adducts 52 and benzothiadiazole derivatives. 53 2,1,3-Benzothiadiazole (BTD) is a new class of fluorescent cell markers, as we have disclosed elsewhere.…”

Construction and Cell Imaging Study of a Novel Fluorescent Probe for ONOO– Detection

“…另外, ONOO -作为一种重要的信号 分子 [5] , 揭示 ONOO -确切的信号传导过程和致病机制 对相关疾病的诊断与治疗具有重要意义 [6] . 因此, 发展 实时、快速和灵敏检测生理环境中的 ONOO -的方法是 十分必要的.目前, 传统检测 ONOO -的方法包括化学发光法、 电化学法、拉曼法和荧光光谱法等 [7][8][9][10] . 荧光探针由于 具有选择性高、灵敏度好和成本低等优点以及对样品可 实现无损实时监测, 已被广泛用于细胞和活体中相关活 性分子的检测与成像 [11][12] .…”