Surface-enhanced Raman spectroscopy detection of protein-ligand binding using D-glucose and glucose binding protein on nanostructured plasmonic substrates

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

“…The quality of fabricated substrates is monitored using scanning electron microscopy (SEM). The standard SEM procedures are described elsewhere 16,17,18,19 and not included in the present protocol. Figure 6 shows representative SEM images of Au and Ag nano-dots and nano-hexagon like structures (panels a-d), as well as non-structured Au and Ag pads (panels e and f, respectively).…”

“…3. Perform Raman sampling 18,19 with a laser intensity between 2.4-3.1 mW with a total duration of less than 20 sec to prevent damage to the sample with an objective of 10X magnification. Acquire Raman spectra for samples from Designs 1, 2, and 3 using the excitation wavelengths as indicated in Table 1.…”