Surface Enhanced Raman Spectroscopy of Proteins: Implications for Drug Designing

Abstract

In this review article we present a general overview of the recent progress in the newly developing area of the study of protein-ligand interaction by surface enhanced Raman spectroscopy (SERS). Since its first observation in 1977, SERS have been fast developing into an analytical tool for trace detection of molecular entities, particularly in the area of bio-molecule sensing and characterization. Also, with the development of the ability to design a variety of plasmonic structures and to be able to control and tune their plasmonic properties, we have been able to use them as SERS substrates for probing complex materials. Here we describe yet another application of SERS, mainly protein-ligand interaction and its future into drug designing. We start with a general description of the SERS phenomenon. Subsequently we discuss the key spectral features of amino acids, peptides and proteins, and their structural aspects that can be elucidated from the SERS spectra. In the final sections we discuss the application of SERS to the study protein-ligand interaction and its potential role in the area of drug designing.

Naphthoquinone-mediated Inhibition of Lysine Acetyltransferase KAT3B/p300, Basis for Non-toxic Inhibitor Synthesis

“…Kinetic Characterization-Lysine acetyltransferase assays were carried out with an ectopically expressed baculoviral construct encoding full-length p300, in the absence or presence of different concentrations of the inhibitor PTK1 (0, 15,20,25,35, and 45 M). The enzyme is known to catalyze a bisubstrate reaction comprising two substrates, namely core histone and [ 3 H]acetyl-CoA.…”

“…SERS is a very sensitive method, and the spectra of the proteins depend on the distance and orientation of the protein on the surface of the nanoparticles. Therefore, this technique gives us insight into the structural changes upon the binding of ligands to proteins (35). The spectra of baculo-expressed full-length p300 (Fig.…”

Formation of copper porous structures under near-equilibrium chemical vapor deposition

“…That is why copper has been selected in this work as a material for comparative analysis. It is necessary to point out, that the choice of copper is also caused by wide application of copper-based porous nanosystems as catalytic materials and biosensors [54][55][56][57][58][59]. Besides that, during the condensation process copper reacts badly with chemically active residual gases as compared to other metals.…”

Revealing the trehalose mediated inhibition of protein aggregation through lysozyme–silver nanoparticle interaction

“…44 Since the electromagnetic field enhancement decays as the third power of the radial distance from the surface, the vibrational modes lying close to the surface undergoes preferential enhancement than those lying afar. 45 Notably, the SERS spectrum of lysozyme-nanoparticle conjugate in 0.05 M trehalose solution displayed key differences in relation to the spectrum acquired in the absence of trehalose. The detailed band assignments are provided in Table 1.…”