Surface fluids effects on the bladder tissue characterisation using electrical impedance spectroscopy

Keshtkar, Ahmad; Mesbahi, Asghar; Mehnati, Parinaz; Keshtkar, Asghar

doi:10.1016/j.medengphy.2007.07.008

Cited by 5 publications

(8 citation statements)

References 11 publications

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“…The total diameter of the probe was only 2 mm and thus it was limited because of the maximum permitted diameter of the endoscopic channel to pass the probe towards the inside of the urinary bladder during the bladder surgery. Detailed information about this probe can be found in Keshtkar et al (2001) and Smallwood et al (2002).…”

Section: Methodsmentioning

confidence: 99%

“…In the urinary bladder, initially an attempt was made to distinguish the malignant (CIS and tumour) and benign (inflamed and normal) areas in the human bladder epithelium using the electrical impedance technique at seven different frequencies (Keshtkar et al 2001). Then, the same technique showed significant differences (p < 0.05 at seven frequencies between 9.6 and 614 kHz) between normal and malignant urothelium (ex vivo), but was unable to classify individual measurements (Smallwood et al 2002, Wilkinson et al 2002.…”

Section: Introductionmentioning

confidence: 99%

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Electrical impedance spectroscopy and the diagnosis of bladder pathology

Keshtkar

Smallwood

2006

Physiol. Meas.

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Bladder pathology is usually investigated visually by cystoscopy. At present, definitive diagnosis of the bladder can be made by biopsy only, usually under general anaesthesia. This is a relatively high-cost procedure in terms of both time and money and is associated with discomfort for the patient and morbidity. Thus, we used an electrical impedance spectroscopy technique for differentiating pathological changes in the urothelium and improving cystoscopic detection. For ex vivo study, a whole or part of the patient's urinary bladder was used to take the readings less than half an hour after excision at room temperature, about 27 degrees C, using the Mk3.5 Sheffield System (2-384 kHz in 24 frequencies). In this study, 145 points (from 16 freshly excised bladders from patients) were studied in terms of their biopsy reports matching to the electrical impedance measurements. For in vivo study, a total of 106 points from 38 patients were studied to take electrical impedance and biopsy samples. The impedance data were evaluated in both malignant and benign groups, and revealed a significant difference between these two groups. The impedivity of the malignant bladder tissue was significantly higher than the impedivity of the benign tissue, especially at lower frequencies (p < 0.001). In addition, the receiver operating characteristic (ROC) curve for impedance measurements indicated that this technique could provide diagnostic information (individual classification is possible). Thus, the authors have investigated the application of bio-impedance measurements to the bladder tissue as a novel and minimally invasive technique to characterize human bladder urothelium. Therefore, this technique, especially at lower frequencies, can be a complementary method for cystoscopy, biopsy and histopathological evaluation of the bladder abnormalities.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrical impedance spectroscopy and the diagnosis of bladder pathology

Keshtkar

Smallwood

2006

Physiol. Meas.

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“…Their work was on a thinner tissue, which in in vivo use will require less pressure. This will ensure that the probe is only contacting the bladder [30,31]. Belmont et al also suggested that when soft tissues are compressed fluid loss must be seen as being composed of both intra-and extra-cellular fluid [32].…”

Section: Pressure Testingmentioning

confidence: 99%

“…Keshtar et al in their study on bladder tissue also suggested that by applying an external pressure to tissues, it is possible to interrupt the blood supply. This causes an increase in impedance as a result of reduction in the volume of fluid in the tissues [30]. Their work was on a thinner tissue, which in in vivo use will require less pressure.…”

Section: Pressure Testingmentioning

confidence: 99%

Fabrication of a Gold Dual‐microelectrode Patterned on a Silicone Substrate Probe for the Discrimination of Different Tissues

et al. 2020

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A gold dual‐microelectrode probe patterned on a silicon substrate for bio‐impedance measurement was fabricated, characterised and tested on different types of tissues. The aim of this study is to fabricate a device capable of using bioimpedance to discriminate different tissue types. This proof of concept of the device was characterised on meat and a gel/lard phantom which mimics fat and muscle. The initial results demonstrated that the gold dual‐microelectrode probe had excellent electrochemical recording potential. The bio‐impedance data derived was capable of differentiation between biological tissues. Bio‐impedance measurement may enhance cancer diagnostics by providing novel data in relation to cancer and surrounding healthy tissue. This manuscript describes the design, fabrication and validation of a gold dual‐microelectrode bioimpedance sensor with a potential future application in cancer diagnostics.

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“…EIS was successfully applied to differentiate normal and neoplastic tissues of skin [12], breast [13], lungs [14], bladder [15] and prostate [16] etc. These malignant tissues either show increase or decrease in conductivity compared to normal tissue depending upon the type of tissue involved [13,16].…”

Section: Introductionmentioning

confidence: 99%

Electrical conductivity spectra of hepatic tumors reflect hepatocellular carcinoma progression in mice

Shetty¹,

Anushree²,

Kumar

et al. 2020

Biomed. Phys. Eng. Express

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Background: Electrical impedance spectroscopy is a technique which evaluates differences in dielectric properties of tissues for cancer identification. Methods: Murine hepatic cancer model was developed by intraperitoneal administration of N-nitrosodiethylamine to male BALB/c mice. Tumors obtained were evaluated for their conductivity in frequency range of (4 Hz–5 MHz). All tumors were subjected to histopathological grading and parameters such as free spacing, necrosis, and cell density were estimated on histological slides. The status of gap junctions and gap junction intercellular communication (GJIC) were studied using enzyme-linked immunosorbent assay, immunohistochemistry, dye transfer assay, and electron microscopy. Results: Histopathological investigation revealed the presence of moderately to poorly-differentiated hepatocellular carcinoma (HCC) in mice. All types of tumors showed higher electrical conductivity than normal liver tissue in frequency range (4 Hz–1 kHz). However, in frequency range (10 kHz–5 MHz) only poorly-differentiated tumors showed higher conductivity compared to normal tissue. The most prominent findings in moderately-differentiated and poorly-differentiated HCC were increased visible free spaces and necrosis respectively. The status of cell gap junctions were significantly deteriorated in tumors and a corresponding significant reduction in GJIC was also observed. These biological indicators were correlated with electrical conductivity of hepatic tumors. Conclusion: Variations in electrical conductivity spectra of hepatic tumors reflect progression of HCC. General significance: Future studies can be planned to perform hierarchical clustering of dielectric parameters with more number of tumor samples to establish dielectric spectroscopy-based classification or staging of hepatic tumors.

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Surface fluids effects on the bladder tissue characterisation using electrical impedance spectroscopy

Cited by 5 publications

References 11 publications

Electrical impedance spectroscopy and the diagnosis of bladder pathology

Electrical impedance spectroscopy and the diagnosis of bladder pathology

Fabrication of a Gold Dual‐microelectrode Patterned on a Silicone Substrate Probe for the Discrimination of Different Tissues

Electrical conductivity spectra of hepatic tumors reflect hepatocellular carcinoma progression in mice

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