Surface functionalization by RF plasma treatment of polymers for immobilization of bioactive-molecules

Narayanan, Pallassana V.

doi:10.1163/156856294x00301

Cited by 20 publications

(9 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Effectiveness of the plasma treatment on the lumenal surface of the PE tubing was evaluated using the capillary-rise (CR) method [7]. Use of the CR method allows us to monitor the uniformity of the contact angle inside the PE tubing as a function of distance along the tubing, which is believed to be very dependent on the lumenal-surface properties.…”

Section: Capillary-rise Methodsmentioning

confidence: 99%

“…The CR measurements of the PE tubing were obtained by cutting the 50-cm long plasma-treated tubing into ten sections of 5-cm length. Each section was then dipped in a beaker of deionized water such that at least 4 cm of the section was submerged [7]. Then the tube was slowly withdrawn from the water, and the height of the water column supported by the surface tension on the lumenal surface was measured.…”

Section: Capillary-rise Methodsmentioning

confidence: 99%

“…However, such reactors are not very practical for surface modification of small diameter tubing [5] and many small-scale biomedical devices [8]. This is because the plasma often does not penetrate along the entire length of the inside (lumenal surface) of the tubing, [6], [7] when the tubing is placed in a large-volume reactor, and thus only small portions near the ends of the tube are treated [5]. Also, complications in treating the side walls of narrow trenches and reservoirs of small-scale biomedical devices can cause further nonuniformities of the coating.…”

mentioning

confidence: 99%

“…This includes, but is not limited to, artificial blood vessels (vascular grafts) of various diameters, as well as catheters. Plasma modification has been used to treat the lumenal surfaces of polyethylene terephthalate [19], [20] (PET, Dacron), expanded polytetrafluoroethylene, [19], [21] (ePTFE), polyethylene [22], [23] (PE), and polyurethane [7] tubes. The traditional method for coating the lumenal surfaces of these polymer tubes uses plasma polymerization (PP) of a monomer gas so as to modify the surface of the material directly or to introduce functional groups that can later be used to immobilize specific biomolecules or proteins.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Control of uniformity of plasma-surface modification inside of small-diameter polyethylene tubing using microplasma diagnostics

Lauer

Shohet

Albrecht

et al. 2005

IEEE Trans. Plasma Sci.

View full text Add to dashboard Cite

Abstract-A hollow-cathode microplasma was used to modify the lumenal surface of small-diameter polyethylene (PE). We make use of two microplasma diagnostics to monitor the plasma properties during the treatment process. A microwave cavity was used to measure the density of the microplasma. Emitted light from the microplasma was fed into a monochromator at various positions along the PE tube to assess uniformity of the microplasma. Effectiveness of plasma treatments were evaluated using the capillary-rise method at various positions along the tubing. We show a correlation between the properties of the inner surface of the PE tubing and the light emitted from the plasma. A Poly(ethylene oxide) (PEO) surfactant was immobilized to the lumenal surface of the PE tubing using the microplasma discharge. An in vitro blood-circulation loop was constructed to test the hematocompatibility of the PE tubes. After blood exposure, scanning electron microscope images were taken to assess the density of adhering platelets along the length of the tubes. The plasma-treated tubing showed fewer blood adherents than the untreated tubing. By suitably controlling the pressure drop along the tube, the uniformity of the microplasma treatment along the tubing can be optimized.

show abstract

Section: Capillary-rise Methodsmentioning

confidence: 99%

Section: Capillary-rise Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Control of uniformity of plasma-surface modification inside of small-diameter polyethylene tubing using microplasma diagnostics

Lauer

Shohet

Albrecht

et al. 2005

IEEE Trans. Plasma Sci.

View full text Add to dashboard Cite

show abstract

“…The decrease of WCA from 119.91 to 18.10 and 1.63°suggested that the PCL scaffold was more hydrophilic when treated with RF plasma and immobilized with the rCF peptide, respectively. The RF plasma of Ar/O 2 discharge created surface hydroxyl and carboxylic acid groups of the scaffolds leading to an increase of their hydrophilicity [42]. When the carboxylic groups were activated with EDC and NHS, and later coupled to the rCF peptide, the PCL-rCF scaffold was very hydrophilic, mainly due to the hydrophilic property of the peptide.…”

Section: Fabrication and Characterization Of Electrospun Scaffoldsmentioning

confidence: 99%

Repetitive Gly-Leu-Lys-Gly-Glu-Asn-Arg-Gly-Asp Peptide Derived from Collagen and Fibronectin for Improving Cell–Scaffold Interaction

Chaisri

Chingsungnoen

Siri

2014

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Suitable scaffolds for tissue engineering should provide a microenvironment for cell dwelling and directing cell behavior that resemble the native environment. Three-dimensional geometry of electrospun scaffolds well supports cell deposition, but they often lack biomacromolecules to induce cell responses. In this work, the repetitive collagen and fibronectin motif (rCF) peptide containing multiple repeats of Gly-Leu-Lys-Gly-Glu-Asn-Arg-Gly-Asp sequence derived from the cell adhesion motifs of collagen and fibronectin was produced as the alternative agent to induce cell-scaffold interaction. The DNA fragment encoding rCF peptide was amplified by a polymerase chain reaction using overlap primers without a DNA template, cloned into a protein expression vector, and expressed as a His-tag fusion peptide in Escherichia coli. The purified rCF peptide possessed cell adhesion activity about 1.5-fold of the commercial RGD peptide. The rCF peptide was grafted onto the electrospun PCL scaffold via RF plasma of Ar/O2 discharge and acrylic acid treatment. The immobilized rCF peptide significantly increased surface hydrophilicity and enhanced cell proliferation of the electrospun PCL scaffold. These findings suggest the potential application of rCF peptide for improving the biomimetic functions of polymeric scaffolds for tissue engineering.

show abstract

Effect of nitrogen and oxygen incorporated into TMSAA plasma coating on surface‐bound heparin activity

Chen

Osaki

Zamora

et al. 2003

J of Applied Polymer Sci

View full text Add to dashboard Cite

ABSTRACT:To investigate the influence of nitrogen and oxygen incorporated into N-trimethylsilylallyamine (TM-SAA) plasma coating on heparin binding to the surfaces, four types of monomer combinations were utilized. Those combinations include TMSAA alone, TMSAA mixed with nitrogen (TMSAA ϩ N 2 ), with air (TMSAA ϩ air), and with oxygen (TMSAA ϩ O 2 ). Fourier transform infrared (FTIR) spectroscopy was employed to study the coating of the bulk structure. The thickness and surface morphology of the coatings were measured using atomic force microscopy (AFM). Electron spectroscopy for chemical analysis (ESCA) and the contact angle were used to investigate the surface elemental composition and hydrophilicity, respectively. It was found that the incorporation of oxygen into the coating formation significantly increased the deposition rate of the TMSAA ϩ O 2 coating, but the heparin activity was the least even though it made the coating surface more hydrophilic. This is considered to have resulted from the loss of nitrogen in the coating structure due to the oxygen replacement to nitrogen. The nitrogen incorporated into the coating had no noticeable effect on the heparin surface-binding ability. The TMSAA ϩ air plasma grafting process exhibited the best heparin attachment to the surface, which could be largely attributed to its highest surface roughness, although the nitrogen composition was decreased to some extent compared to the pure TMSAA plasma coating.

show abstract

Surface functionalization by RF plasma treatment of polymers for immobilization of bioactive-molecules

Cited by 20 publications

References 7 publications

Control of uniformity of plasma-surface modification inside of small-diameter polyethylene tubing using microplasma diagnostics

Control of uniformity of plasma-surface modification inside of small-diameter polyethylene tubing using microplasma diagnostics

Repetitive Gly-Leu-Lys-Gly-Glu-Asn-Arg-Gly-Asp Peptide Derived from Collagen and Fibronectin for Improving Cell–Scaffold Interaction

Effect of nitrogen and oxygen incorporated into TMSAA plasma coating on surface‐bound heparin activity

Contact Info

Product

Resources

About