Surface functionalization of silica-coated magnetic nanoparticles for covalent attachment of cholesterol oxidase

Šulek, Franja; Drofenik, Miha; Habulin, Maja; Knez, Željko

doi:10.1016/j.jmmm.2009.07.075

Cited by 103 publications

(64 citation statements)

References 34 publications

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“…Particle Characterization. As mentioned before, maghemite (γ-Fe 2 O 3 ) nanoparticles were prepared by the coprecipitation technique described by Šulek et al [22,23]. In their method (in the rest of the article called method 1), a stirred tank batch reactor equipped with a magnetic/heater was used.…”

Section: Resultsmentioning

confidence: 99%

“…In ) were synthesized according to the procedure described by Šulek et al [22,23]. In order to improve process, the synthesis in this work was performed in oil bath using a stirredtank batch reactor to avoid precipitation of magnetic nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

“…Surface Activation and Enzyme Immobilization. The surface of chemically modified maghemite nanoparticles was then subject to activation [20,22,23]. Briefly, MNPs were washed with deionized water prior to exposure to 2% glutaraldehyde aqueous solution for 2 h. After the surface activation, MNPs were washed out with deionized water and the enzyme was introduced (γ ADH = 0.08 mg/mL) to attach during the 28 h. These conditions were determined to be optimal using evolutionary operation method (EVOP) [24][25][26] (data not shown).…”

Section: Introductionmentioning

confidence: 99%

“…Analytics. In order to identify and characterize magnetic nanoparticles obtained in this work, different methods were used (power X-ray diffraction to identify the particles, vibrating sample magnetometer to determine magnetization, Fourier transform infrared (FT-IR) spectrum to record aminosilane, atomic absorption spectrophotometer to determine iron [22,23], and ultraviolet-visible (UV-VIS) spectrophotometer to determine ferrous iron [27]). …”

Section: Introductionmentioning

confidence: 99%

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NADH Oxidation in a Microreactor with an Oscillating Magnetic Field

Šalić¹,

Pindrić²,

Podrepšek³

et al. 2016

J Flow Chem

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In this study, magnetic nanoparticles (MNPs) of maghemite (γ-Fe 2 O 3 ) were synthesized and characterized. The method of multifactor experimental design and evolutionary operation (EVOP) was used to optimize immobilization of the alcohol dehydrogenase (ADH) enzyme on MNPs. Optimal operating conditions for the immobilization process were determined (γ ADH = 0.08 mg/mL, 2% glutaraldehyde for surface activation, t = 28 h), and in such conditions, a specific activity of S.A. = 118 ± 6 U/mg and immobilization efficiency of η = 84.97 ± 3.67% were achieved. Compared to the native enzyme, ADH immobilized on MNPs retained 66.45 ± 3.66% of the initial activity. ADH immobilized on MNPs at optimal conditions was used as a biocatalyst for model reaction -NADH oxidation. NADH oxidation was performed in two different magnetic microreactor configurations: (1) microreactor equipped with permanent square magnets and (2) microreactor equipped with an electromagnet and an oscillating magnetic field that enables magnetic particles movement in the microreactor. In the system with the oscillating magnetic field, equal conversion (X = 100%) was achieved in 2-fold shorter residence time.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

NADH Oxidation in a Microreactor with an Oscillating Magnetic Field

Šalić¹,

Pindrić²,

Podrepšek³

et al. 2016

J Flow Chem

View full text Add to dashboard Cite

show abstract

“…This unique feature of silane coupling agents has made silanation a widely used method in modifying surface properties and introducing functional groups on particles. A large volume of literature is available for the surface functionalization of magnetic silica particles by silanation (Berry & Curtis, 2003;Dong et al, 2008;Koneracka et al, 1999;Sulek et al).…”

Section: Application Of Magnetic Nanoparticlesmentioning

confidence: 99%