Surface hydrophobicity and roughness influences the morphology and biochemistry of streptomycetes during attached growth and differentiation

Abstract

Streptomycetes, soil-dwelling mycelial bacteria, can colonise surface of organic soil debris and soil particles. We analysed the effects of two different inert surfaces, glass and zirconia/silica, on the growth and antibiotic production in Streptomyces granaticolor. The surfaces used were in the form of microbeads and were surrounded by liquid growth media. Following the production of the antibiotic granaticin, more biomass was formed as well as a greater amount of antibiotic per milligram of protein on the glass beads than on the zirconia/silica beads. Comparison of young mycelium (6 h) proteomes, obtained from the cultures attached to the glass and zirconia/silica beads, revealed three proteins with altered expression levels (dihydrolipoamide dehydrogenase, amidophosphoribosyltransferase and cystathionine beta-synthase) and one unique protein (glyceraldehyde-3-phosphate dehydrogenase) that was present only in cells grown on glass beads. All of the identified proteins function primarily as cytoplasmic enzymes involved in different parts of metabolism; however, in several microorganisms, they are exposed on the cell surface and have been shown to be involved in adhesion or biofilm formation.

Effect of the combination of several irrigants on dentine surface properties, adsorption of chlorhexidine and adhesion of microorganisms to dentine

“…Statistical correlation tests were not performed with the experimental data from the measurements of roughness, wettability and adhesion of microorganisms because the dentine samples used in the different sets of experiments were not the same, although they were prepared identically. The increase in roughness Irrigants, dentine and microorganism's adhesion Tartari et al and wettability is shown to increase the adhesion of microorganisms on surfaces (Mei et al 2011, Tang et al 2011, Petrackova et al 2013. However, the effects of roughness and wettability on the adhesion of the tested microorganisms could not be established by this study, as previously described (Burgers et al 2010, do Nascimento et al 2013.…”

“…, Petrackova et al . ). However, the effects of roughness and wettability on the adhesion of the tested microorganisms could not be established by this study, as previously described (Burgers et al .…”

Monophyletic group of unclassified γ- Proteobacteria dominates in mixed culture biofilm of high-performing oxygen reducing biocathode

“…To establish conclusions on the role of the modification further parallel and duplicated experiments are necessary and will be carried out. In many cases biofilm development of wastewater and soil isolates was shown to be increased on hydrophilic surfaces [49][50][51], but, in contrast, unmodified and to a lesser extent more hydrophobic surfaces were preferred by the dominant bacterial strains in this study resulting in thicker biofilms. In the work by Petrackova et al [51] Streptomycetes were also found to colonize hydrophobic surfaces but this resulted in an altered protein expression.…”

“…In many cases biofilm development of wastewater and soil isolates was shown to be increased on hydrophilic surfaces [49][50][51], but, in contrast, unmodified and to a lesser extent more hydrophobic surfaces were preferred by the dominant bacterial strains in this study resulting in thicker biofilms. In the work by Petrackova et al [51] Streptomycetes were also found to colonize hydrophobic surfaces but this resulted in an altered protein expression. A similar phenomenon might also play a role in the selectivity of the surfaces used in this work for the specific group of γ-Proteobacteria, which were initially inoculated in equal amounts by using the same sludge.…”

Role of Phenotypic Switching in Stability and Persistence of Pseudomonas aeruginosa Biofilms

“…The surface roughness seems to be a characteristic feature of biofilm indwellers. It has been reported that cells with rough appearance are more hydrophobic and these phenotypes exhibit strong surface colonization and adhesion abilities [22]. Kaiser [23] described those bacteria which showed elongated cells, have the competitive advantage of the colony edge.…”