Surface-immobilized polyethylene oxide for bacterial repellence

Desai, Neil; Hossainy, Syed; Hubbell, Jeffrey A.

doi:10.1016/0142-9612(92)90160-p

Cited by 187 publications

(116 citation statements)

References 13 publications

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“…Antimicrobial coatings are required to have antibacterial efficacy, ease of fabrication and low toxicity. Copper and copper alloy surfaces, 34,35 bacteriophage modified surfaces, 36 polymers, [37][38][39] polycationic modified surfaces, [40][41][42] photosensitiser coated surfaces 43,44 as well as silver and silver containing surfaces 45,46 have been extensively used as antimicrobial coatings, albeit increasing exposure of these materials to microbes eventually leads to the increased occurrence of resistance to treatment. In our study, cefaclor reduced gold nanoparticles have been used to coat poly(ethyleneimine) (PEI) modified glass surfaces to obtain highly effective and robust antimicrobial coatings.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic mediated synthesis of gold nanoparticles with potent antimicrobial activity and their application in antimicrobial coatings

2010

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We report a one-pot synthesis of spherical gold nanoparticles (52-22 nm) and their capping with cefaclor, a second-generation antibiotic, without use of other chemicals. The differently sized gold nanoparticles were fabricated by controlling the rate of reduction of gold ions in aqueous solution by varying the reaction temperature (20-70 C). The primary amine group of cefaclor acted as both the reducing and capping agent for the synthesis of gold nanoparticles leaving the b-lactam ring of cefaclor available for activity against microbes. Antimicrobial testing showed that cefaclor reduced gold nanoparticles have potent antimicrobial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria as compared to cefaclor or gold nanoparticles alone. The minimum inhibition concentrations (MICs) of cefaclor reduced gold nanoparticles were 10 mg mL À1and 100 mg mL À1 for S. aureus and E. coli respectively. The cefaclor reduced gold nanoparticles were further coated onto poly(ethyleneimine) (PEI) modified glass surfaces to obtain antimicrobial coatings suitable for biomedical applications and were tested against E. coli as an exemplar of activity. The antimicrobial coatings were very robust under adverse conditions (pH 3 and 10), inhibited the growth of E. coli on their surfaces, and could be used many times with retained activity. Results from a combined spectroscopic (FTIR) and microscopic study (AFM) suggest that the action of these novel particles is through the combined action of cefaclor inhibiting the synthesis of the peptidoglycan layer and gold nanoparticles generating ''holes'' in bacterial cell walls thereby increasing the permeability of the cell wall, resulting in the leakage of cell contents and eventually cell death.

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Section: Introductionmentioning

confidence: 99%

Antibiotic mediated synthesis of gold nanoparticles with potent antimicrobial activity and their application in antimicrobial coatings

2010

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“…Bacterial characteristics known to influence adhesion are hydrophobicity, zeta potential (Bos et al, 1999), motility (Kogure et al, 1998), and release of extracellular substances, such as polysaccharides (Azeredo et al, 1999), proteins (Dufrene et al, 1996) and biosurfactants (Van Hoogmoed et al, 2000). Relevant properties of the substratum surface are hydrophobicity, zeta potential (Bos et al, 1999), and surface texture (Desai et al, 1992;Holland et al, 1998). The influence of the surface free energies of the substratum and the bacterium can be modelled using a thermodynamic approach (Bos et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Bacterial factors influencing adhesion of Pseudomonas aeruginosa strains to a poly(ethylene oxide) brush

et al. 2006

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Most bacterial strains adhere poorly to poly(ethylene oxide) (PEO)-brush coatings, with the exception of a Pseudomonas aeruginosa strain. The aim of this study was to find factors determining whether P. aeruginosa strains do or do not adhere to a PEO-brush coating in a parallel plate flow chamber. On the basis of their adhesion, a distinction could be made between three adhesive and three non-adhesive strains of P. aeruginosa, while bacterial motilities and zeta potentials were comparable for all six strains. However, water contact angles indicated that the adhesive strains were much more hydrophobic than the non-adhesive strains. Furthermore, only adhesive strains released surfactive extracellular substances, which may be engaged in attractive interactions with the PEO chains. Atomic force microscopy showed that the adhesion energy, measured from the retract curves of a bacterial-coated cantilever from a brush coating, was significantly more negative for adhesive strains than for non-adhesive strains (P<0?001). Through surface thermodynamic and extended-DLVO (Derjaguin, Landau, Verwey, Overbeek) analyses, these stronger adhesion energies could be attributed to acid-base interactions. However, the energies of adhesion of all strains to a brush coating were small when compared with their energies of adhesion to a glass surface. Accordingly, even the adhesive P. aeruginosa strains could be easily removed from a PEO-brush coating by the passage of a liquid-air interface. In conclusion, cell surface hydrophobicity and surfactant release are the main factors involved in adhesion of P. aeruginosa strains to PEO-brush coatings.

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“…6 Due to the unique ability of PEG to block serum protein adsorption as well as the ability to prevent prokaryotic and eukaryotic cell adhesion, PEG can be further used as a surface coating material for the passivation of biomaterial surfaces. [7][8][9] The non-fouling character of such coatings relies on steric repulsion effects between the protein and the hydrated polymer chains at the surface as well as on parameters such as chain length and surface density. 10 Further studies proved the prevention of unspecific protein adsorption using an ultrathin star-shaped PEG layer.…”

Section: Introductionmentioning

confidence: 99%

Cell phenotypic changes of mouse connective tissue fibroblasts (L-929) to poly(ethylene glycol)-based gels

et al. 2013

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a Cellular responses to various gels fabricated by photoinitiated crosslinking using acrylated linear and multi-arm poly(ethylene glycol) (PEG)-based and poly(propylene glycol)-b-poly(ethylene glycol) precursors were investigated. While no protein adsorption and cell adhesion were observed on the hydrophilic PEG-based gels, protein adsorption and cell adhesion did occur on the more hydrophobic gel generated from the block copolymer precursor. Murine fibroblast viability on the poly(ethylene glycol)-based gels was studied in the course of 72 h and the results indicated no cytotoxicity. In a systematic study, extraand intracellular metabolites of the murine fibroblasts cultured on these PEG-based gels were examined by GC-MS. Distinct intra-and extracellular changes in primary metabolism, namely amino acid metabolism, glycolysis and fatty acid metabolism, were observed. Cells cultured on the polymeric gels induced more intense intracellular changes in the metabolite profile by means of higher metabolite intensities with time in comparison to cells cultured on the reference substrate (tissue culture polystyrene). In contrast, extracellular changes of metabolite intensities were comparable.

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Surface-immobilized polyethylene oxide for bacterial repellence

Cited by 187 publications

References 13 publications

Antibiotic mediated synthesis of gold nanoparticles with potent antimicrobial activity and their application in antimicrobial coatings

Antibiotic mediated synthesis of gold nanoparticles with potent antimicrobial activity and their application in antimicrobial coatings

Bacterial factors influencing adhesion of Pseudomonas aeruginosa strains to a poly(ethylene oxide) brush

Cell phenotypic changes of mouse connective tissue fibroblasts (L-929) to poly(ethylene glycol)-based gels

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