Surface markers of cloned human alloreactive T lymphocytes

Chardonnens, X; Froment, Yvette; Joye, Marie-Thérèse; Jeannet, M

doi:10.1007/bf00364341

Cited by 4 publications

(1 citation statement)

References 8 publications

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“…Established methods of studying the clonal proliferation of human T lymphocytes are the one-step agar colony method [5,10] and the further expansion of individual T-cell colonies [3,9,14]. These methods provide useful tools for studying the response of cloned T lymphocytes to polyclonal stimuli and to recall antigens.…”

Section: S0nderstrup-hansen Laboratory Of Medical Immunology Ta mentioning

confidence: 99%

Colony Formation by Subpopulations of Human T Lymphocytes

Sønderstrup-Hansen

Petersen

1984

Scand J Immunol

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Twelve human T-cell lines were derived from cultures stimulated with phytohaemagglutinin (PHA) or with the recall antigen purified protein derivative (PPD), using the one-step agar colony method, followed by further expansion of individual T-cell colonies for at least 50 days. As judged by monoclonal antibodies, two of the PHA-derived T-cell lines carried the helper-inducer (H-I) phenotype and two the suppressor-cytotoxic (S-C) phenotype. Four PPD-derived cell lines displayed the H-I phenotype, and three of these proliferated specifically when challenged with the recall antigen, whereas the fourth did not. Four PPD-induced T-cell lines carried the S-C phenotype, and only one of these was antigen-specific. All cell lines with H-I phenotype displayed helper cell activity as determined by Ig secretion of B lymphocytes stimulated with a T-cell-dependent polyclonal B-cell activator. Cell lines with the S-C phenotype had no such helper activity and suppressed Ig responses in the presence of freshly isolated T cells. Thus, a good correlation between phenotypic markers and functions was found. T helper cells and T suppressor cells can be differentiated by means of both polyclonal and antigenic stimuli.

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Section: S0nderstrup-hansen Laboratory Of Medical Immunology Ta mentioning

confidence: 99%

Colony Formation by Subpopulations of Human T Lymphocytes

Sønderstrup-Hansen

Petersen

1984

Scand J Immunol

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Clonal expression of differentiation and Ia‐like antigens on alloreactive human T lymphocytes

et al. 1984

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A series of human T lymphocyte clones showing specific responses for major histocompatibility complex-encoded alloantigens were isolated from a single culture. They were classified into distinct functional groups based on measurement of alloantigen-specific proliferation and cytotoxicity. Surface antigen expression on these clones was analyzed using a panel of monoclonal antibodies specific for T cell differentiation antigens and for Ia-like antigens. Four distinct groups were identified based on the Leu series of differentiation antigens: (a) Leu1+,2+,3+,4+; (b) Leu1+,3+,4+; (c) Leu1+,2+,4+; and (d) Leu2+,4+. Functionally distinct clones showed not only differential expression of Leu2 and Leu3 , antigens which had previously been shown to distinguish helper/inducer and cytotoxic/suppressor cells, but also of Leu1, an antigen classified as a common T cell marker. All of the clones were found to express Ia antigens as detected by three framework monoclonal antibodies, but none were found to express a determinant associated with the DR3 allospecificity , although it was found on normal B lymphocytes and a B cell line established from the HLA-DR3 individual from whom these clones were derived.

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