Surface modification for controlled cell growth on copolymers of N-isopropylacrylamide

Rochev, Yuri; Golubeva, T.; Gorelov, Alexander; Allen, Lorcan T.; Gallagher, William M.; Селезнева, И. И.; Gavrilyuk, B. K.; Dawson, Kenneth A.

doi:10.1007/3-540-45725-9_35

Cited by 27 publications

(31 citation statements)

References 6 publications

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“…Through increasing addition of the more hydrophobic monomer NtBAAm, we were able to alter the bulk hydrophobic nature of these copolymer films. This alteration in bulk hydrophobicity was confirmed by microcalorimetric measurements and is reflected in the lowering of the lower critical solution temperature, which ranges from 25.1°C for the most hydrophilic (85:15) copolymer, down to 9.8°C for the most hydrophobic (50:50) copolymer (5).…”

mentioning

confidence: 61%

“…NIPAAm-based copolymer films have been previously used as adaptive cell culture substrates (5,6) and, latterly by us and other groups, as prototypical drug delivery systems (7,8). These ''responsive'' copolymers, although not known for their biocompatibility, are relatively benign with respect to interaction with cells, and make for an interesting case study from a practical point of view.…”

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confidence: 99%

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Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect

Allen

Fox

Blute

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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The assessment of biomaterial compatibility relies heavily on the analysis of macroscopic cellular responses to material interaction. However, new technologies have become available that permit a more profound understanding of the molecular basis of cellbiomaterial interaction. Here, both conventional phenotypic and contemporary transcriptomic (DNA microarray-based) analysis techniques were combined to examine the interaction of cells with a homologous series of copolymer films that subtly vary in terms of surface hydrophobicity. More specifically, we used differing combinations of N-isopropylacrylamide, which is presently used as an adaptive cell culture substrate, and the more hydrophobic, yet structurally similar, monomer N-tert-butylacrylamide. We show here that even discrete modifications with respect to the physiochemistry of soft amorphous materials can lead to significant impacts on the phenotype of interacting cells. Furthermore, we have elucidated putative links between phenotypic responses to cell-biomaterial interaction and global gene expression profile alterations. This case study indicates that high-throughput analysis of gene expression not only can greatly refine our knowledge of cell-biomaterial interaction, but also can yield novel biomarkers for potential use in biocompatibility assessment.

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confidence: 61%

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confidence: 99%

Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect

Allen

Fox

Blute

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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“…The LCST of these poly-N-isopropylacrylamide -N-tert-butylacrylamide (pNIPAM-co-NtBA) thermoresponsive copolymers can be decreased by increasing the NtBA fraction. The importance of this study lies in the fact that these pNIPAM-co-NtBA copolymers are currently being investigated as potential drug elution matrices and for cell culture applications [16][17][18][19][20]. Because these copolymers are relatively hydrophilic and will uptake water, it can be difficult to characterise the materials using conventional techniques like Contact Angle Measurements.…”

Section: Introductionmentioning

confidence: 99%

Polarity Assessment of Thermoresponsive Poly(NIPAM-co-NtBA) Copolymer Films Using Fluorescence Methods

et al. 2010

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The in-situ, non-contact, and non-destructive measurement of the physicochemical properties such as the polarity of thin (nm to µm), hydrophilic polymer films is desirable in many areas of polymer science. Polarity is a complex factor and encompasses a range of noncovalent interactions including dipolarity/polarizability and hydrogen bonding. A polarity measurement method based on fluorescence would be ideal, but the key challenge is to identify suitable probes which can accurately measure specific polarity related parameters. In this manuscript we assess a variety of fluorophores for measuring the polarity of a series of relatively hydrophilic, thermoresponsive N-isopropylacrylamide/N-tert-butylacrylamide (NIPAM/NtBA) copolymers. The emission properties of both pyrene and 3-Hydroxyflavone (3-HF) based fluorophores were measured in dry polymer films. In the case of pyrene, a relatively weak, linear relationship between polymer composition and the ratio of the first to the third vibronic band of the emission spectrum (I 1 /I 3 ) is observed, but pyrene emission is very sensitive to temperature and thus not suitable for robust polarity measurements. The 3-

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“…These NIPAM/NtBA copolymers are currently being investigated as potential drug elution matrices and for cell culture applications. [14][15][16] To better understand the physicochemical performance of these hydrophilic polymers it is necessary to accurately measure the polarity of these materials. Polarity in its broadest sense will impact on aspects of polymer behavior such as water uptake, processability, surface energy, drug elution profiles, and mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Measuring the Micro-Polarity and Hydrogen-Bond Donor/Acceptor Ability of Thermoresponsive N-Isopropylacrylamide/N-tert-Butylacrylamide Copolymer Films Using Solvatochromic Indicators

et al. 2009

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Thin polymer films are important in many areas of biomaterials research, biomedical devices, and biological sensors. The accurate in situ measurement of multiple physicochemical properties of thin polymer films is critical in understanding biocompatibility, polymer function, and performance. In this work we demonstrate a facile spectroscopic methodology for accurately measuring the micro-polarity and hydrogen-bond donor/acceptor ability for a series of relatively hydrophilic thermoresponsive copolymers. The micro-polarity of the N-isopropylacrylamide (NIPAM) and N-tert-butylacrylamide (NtBA) co-polymers was evaluated by means of the ET(30), α, β, and π* empirical solvatochromic polarity parameters. The data shows that increasing the NtBA fraction in the dry copolymer film reduces polarity and hydrogen-bonding ability. Within the Kamlet–Taft polarity framework, the NIPAM/NtBA copolymer films are strong hydrogen-bond acceptors, strongly dipolar/polarizable, and rather moderate hydrogen-bond donors. This characterization provides a more comprehensive physicochemical description of polymers, which aids the interpretation of film performance. Comparison of the measured ET(30) values with literature data for other water-soluble polymers show that dry NIPAM/NtBA copolymers are slightly more polar than poly(ethylene oxide), less polar than polyvinyl-alcohol, and approximately the same polarity as poly(N-vinyl-2-pyrrolidone). These findings indicate that this spectroscopic method is a facile, rapid, and nondestructive methodology for measuring polymer properties in situ, suitable for most biomaterials research laboratories.

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Surface modification for controlled cell growth on copolymers of N-isopropylacrylamide

Cited by 27 publications

References 6 publications

Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect

Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect

Polarity Assessment of Thermoresponsive Poly(NIPAM-co-NtBA) Copolymer Films Using Fluorescence Methods

Measuring the Micro-Polarity and Hydrogen-Bond Donor/Acceptor Ability of Thermoresponsive N-Isopropylacrylamide/N-tert-Butylacrylamide Copolymer Films Using Solvatochromic Indicators

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