Surface Modification of Acrylonitrile Fibers and Membrane by Nitrilase from &lt;i&gt;Escherichia Coli BL21 (DE3)/pET-Nit&lt;/i&gt;

Feng, Li Li; Zhang, Jian Fei; Luo, Hui; Li, Zheng

doi:10.4028/www.scientific.net/amr.175-176.651

Cited by 4 publications

(1 citation statement)

References 6 publications

(6 reference statements)

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“…The staining depth of the PAN fabrics was substantially improved when treated by this catalyst. The nitrilase from E. coli BL21 (DE3)/pET-Nit can also effectively modify the surface of PAN fibers and membrane [163]. The hydrophilicity and fabric-dyeing efficiency of these fibers were obviously increased and yet the strength of treated fiber decreased by only 1.17%.…”

Section: Introductionmentioning

confidence: 99%

Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

et al. 2012

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Over the past decades, nitrilases have drawn considerable attention because of their application in nitrile degradation as prominent biocatalysts. Nitrilases are derived from bacteria, filamentous fungi, yeasts, and plants. In-depth investigations on their natural sources function mechanisms, enzyme structure, screening pathways, and biocatalytic properties have been conducted. Moreover, the immobilization, purification, gene cloning and modifications of nitrilase have been dwelt upon. Some nitrilases are used commercially as biofactories for carboxylic acids production, waste treatment, and surface modification. This critical review summarizes the current status of nitrilase research, and discusses a number of challenges and significant attempts in its further development. Nitrilase is a significant and promising biocatalyst for catalytic applications.

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Section: Introductionmentioning

confidence: 99%

Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

et al. 2012

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Nitrile‐converting enzymes: An eco‐friendly tool for industrial biocatalysis

Ramteke

Maurice

Joseph

et al. 2013

Biotech and App Biochem

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Nitriles are organic compounds bearing a − C ≡ N group; they are frequently known to occur naturally in both fauna and flora and are also synthesized chemically. They have wide applicability in the fields of medicine, industry, and environmental monitoring. However, the majority of nitrile compounds are considered to be lethal, mutagenic, and carcinogenic in nature and are known to cause potential health problems such as nausea, bronchial irritation, respiratory distress, convulsions, coma, and skeletal deformities in humans. Nitrile-converting enzymes, which are extracted from microorganisms, are commonly termed nitrilases and have drawn the attention of researchers all over the world to combat the toxicity of nitrile compounds. The present review focuses on the utility of nitrile-converting enzymes, sources, classification, structure, properties, and applications, as well as the future perspective on nitrilases.

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Metagenomic technology and genome mining: emerging areas for exploring novel nitrilases

Gong

Lü

et al. 2013

Appl Microbiol Biotechnol

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Nitrilase is one of the most important members in the nitrilase superfamily and it is widely used for bioproduction of commodity chemicals and pharmaceutical intermediates as well as bioremediation of nitrile-contaminated wastes. However, its application was hindered by several limitations. Searching for new nitrilases and improving their application performances are the driving force for researchers. Genetic data resources in various databases are quite rich in post-genomic era. Besides, more than 99 % of microbes in the environment are unculturable. Metagenomic technology and genome mining are thus becoming burgeoning areas and provide unprecedented opportunities for searching more useful novel nitrilases due to the abundance of already existing but unexplored gene resources, namely uncharacterized genome information in the database and unculturable microbes in the natural environment. These techniques seem to be innovative and highly efficient. This study reviews the current status and future directions of metagenomics and genome mining in nitrilase exploration. Moreover, it discussed their utilization in coping with the challenges for nitrilase application. In the next several years, with the rapid development of nitrile biocatalysis, these two techniques would be bound to attract increasing attentions and even become a dominant trend for finding more novel nitrilases. Also, this review would provide guidance for exploitation of other commercially important enzymes.

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Surface Modification of Acrylonitrile Fibers and Membrane by Nitrilase from <i>Escherichia Coli BL21 (DE3)/pET-Nit</i>

Cited by 4 publications

References 6 publications

Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

Nitrile‐converting enzymes: An eco‐friendly tool for industrial biocatalysis

Metagenomic technology and genome mining: emerging areas for exploring novel nitrilases

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