Surface modification of cell culture carriers: Routes to anhydride functionalization of polystyrene

Nitschke, Mirko; Ricciardi, Serena; Gramm, Stefan; Zschoche, Stefan; Herklotz, Manuela; Rivolo, Paola; Werner, Carsten

doi:10.1016/j.colsurfb.2011.09.034

Cited by 16 publications

(7 citation statements)

References 20 publications

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“…2). The increased oxygen on surface could increase the surface activity, a potential benefit to the growth of biological cells [13,[27][28][29][30][31].…”

Section: Surface Morphology By Afmmentioning

confidence: 99%

“…Hepatocytes are major cells in the mammalian liver responsible for protein synthesis (serum albumin, fibrinogen, lipoproteins and glycoproteins etc.) [9]; carbohydrate metabolisms such as gluconeogenesis and lipogenesis; detoxifications, exogenous metabolisms (diazepam), endogenous metabolisms (steroids), drainage of the intestinal venous blood, and modifications of ammonia into urea for excretion [10][11][12][13]. The culture follows our own established protocol for a period of 5 days.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication and characterization of polymethylmethacrylate (PMMA) thin film by plasma polymerization used for cell culture

Hsieh

et al. 2014

Surface and Coatings Technology

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“…2). The increased oxygen on surface could increase the surface activity, a potential benefit to the growth of biological cells [13,[27][28][29][30][31].…”

Section: Surface Morphology By Afmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of polymethylmethacrylate (PMMA) thin film by plasma polymerization used for cell culture

Hsieh

et al. 2014

Surface and Coatings Technology

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“…In contrast, B cell lines or B cell hybridomas are generally cultivated in tissue culture flasks commonly fabricated from polystyrene. While polystyrene‐based disposable cell culture ware shows adequate performance for most cell culture applications, its surface is difficult to functionalize chemically in a defined way . Thus, a defined substrate mediated cell guidance is difficult to achieve with polystyrene.…”

Section: Discussionmentioning

confidence: 99%

Transparent Substrates Prepared From Different Amorphous Polymers Can Directly Modulate Primary Human B cell functions

Roch

Hahne

Kratz

et al. 2017

Biotechnology Journal

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Manipulation of B cell functions such as antibody and cytokine secretion, is of clinical and biotechnological interest and can be achieved by soluble ligands activating cell surface receptors. Alternatively, the exposure to suitable solid substrates would offer the possibility to transiently induced cell signaling, since the signaling is interrupted when the cells are removed from the substrate. Cell/substrate interactions are mediated by physical valences such as, hydrogen bonds or hydrophobic forces on the substrate surface. Therefore, in this study B cells were cultivated on polymeric substrates, differing in their chemical composition and thus their capacity to undergo physical interactions. Activated B cells cultivated on polystyrene (PS) showed an altered cytokine response indicated by increased IL-10 and decreased IL-6 secretion. Interestingly, B cells cultivated on polyetherurethane (PEU), which has among all tested polymers the highest potential to form strong hydrogen bonds showed an impaired activation, which could be restored by re-cultivation on tissue culture polystyrene. The results indicate that B cell behavior can transiently be manipulated solely by interacting with polymeric surface, which could be explained by receptor activation mediated by physical interaction with the substrate or by altering the availability of the soluble stimulatory reagents by adsorption processes.

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“…Because hMSCs are multipotent and can be expanded in culture, there has been much attention in their clinical potential for tissue repair and gene therapy [10,38,46,47] has poor biocompatibility for cell culture, which makes it necessary to be chemically or physically modified before its application for culturing adherent cells [4,25,35,37]. Hence, there is a great need to develop an alternative surface for hMSCs expansion, screening and assessment.…”

Section: Discussionmentioning

confidence: 99%

The influence of polymer scaffolds on cellular behaviour of bone marrow derived human mesenchymal stem cells

Wang

Kratz

et al. 2012

Clinical Hemorheology and Microcirculation

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Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types. Therefore, they are widely explored in regenerative medicine. The interaction of MSCs with biomaterials is of great importance for cell proliferation, differentiation and function, and can be strongly influenced by numerous factors, such as the chemical nature and the mechanical properties of the material surface. In this study, we investigated the interaction of bone marrow derived human MSCs with different amorphous and transparent polymers namely polystyrene (PS), polycarbonate (PC), poly(ether imide) (PEI), polyetherurethane (PEU) and poly(styrene-co-acrylonitrile) (PSAN). To ensure that the MSCs were solely in contact to the testing material we applied polymeric inserts, which were prepared from the aforementioned polymers via injection molding. The explored inserts exhibited a similar wettability with advancing contact angles ranging from 84±7 o (PEU) to 99±5 o (PS) and a surface roughness of R q  0.86 µm. The micromechanical properties determined by AFM indentation varied from 6±1 GPa (PEU) to 24±5 GPa (PSAN). Cells presented different adhesion rates on the polymer surfaces 24 hours after cell seeding (45±7% (PS), 63±1% (PC), 75±4% (PEI), 69±2% (PEU) and 61±5% (PSAN)). The cells could proliferate on the polymer surfaces, and the fold change of cell number after 16 days of culture reached to 1.93±0.07 (PS), 3.38±0.11 (PC), 3.65±0.04 (PEI), 2.24±0.15 (PEU) and 3.36±0.09 (PSAN). Differences in cell apoptosis could be observed during the culture. After 7 days, the apoptosis of cells on PC, PEI and PSAN decreased to a level comparable to that on standard tissue culture plate (TCP). All of the tested polymers exhibited low cytotoxicity and allowed high cell viability. Compared to cells on TCP, cells on PC and PEI showed similar morphology, distribution as well as F-actin cytoskeleton organization, whereas cells on PSAN were distributed less evenly and cells on PEU were less oriented. Cells were more likely to form clusters on PS. Conclusively, we demonstrated the influence of polymer scaffolds on the cellular behaviour of MSCs, which could be included in the development of novel design concepts based on polymeric biomaterials.

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Surface modification of cell culture carriers: Routes to anhydride functionalization of polystyrene

Cited by 16 publications

References 20 publications

Fabrication and characterization of polymethylmethacrylate (PMMA) thin film by plasma polymerization used for cell culture

Fabrication and characterization of polymethylmethacrylate (PMMA) thin film by plasma polymerization used for cell culture

Transparent Substrates Prepared From Different Amorphous Polymers Can Directly Modulate Primary Human B cell functions

The influence of polymer scaffolds on cellular behaviour of bone marrow derived human mesenchymal stem cells

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