Surface modification of mammalian cells with stimuli-responsive polymers

Abstract

In order to introduce alternative functions into mammalian cells and control them under ambient conditions, poly(N-isopropyl acrylamide) (PNIPAM) was immobilized on the cell surface. Cellular aggregation could be regulated by temperature change. In addition, separation of PNIPAM-conjugated glycoproteins was successfully performed.

Amphiphilic Macromolecules on Cell Membranes: From Protective Layers to Controlled Permeabilization

“…Biocompatibility issues have motivated the development of stimuli-responsive polymers of diverse chemical structures, in order to optimize responses in serum (Francis MF et al 2001) for cell agglomeration (Iwasaki Y et al 2013) permeabilization of mammalian cells, or biocide activity (see Figure 2 for illustrations on T-responsive and light-responsive polymers, or for pH-triggered ones: (Henry SM et al 2006), (Lackey CA et al 1999), (Eccleston ME et al 2000), (Kusonwiriyawong C et al 2003)). The exact origin of stimuli-controlled membrane disruption is to date largely conjectural.…”

“…Antimicrobial agents with selectivity modulated by side groups, (Kuroda K et al 2009;Palermo EF et al 2009a;Palermo EF et al 2011;Palermo EF et al 2009b) Pegylated poly(lysine) (or not shown poly(ethylene imine)) for mild cell adhesion, and LbL coating. (Mansouri S et al 2011), (Wilson JT et al (Ho VHB et al 2011)) or pH-responsive endosomal escape in mammalian cells (poly(acrylic acid) derivatives from (Henry SM et al 2006), (Yessine MA et al 2004)), or poly(lysylphtalate) derivatives with pH-sensitive hemolytic activity (Chen R et al 2009) Temperature-triggered biocide, (Iwasaki Y et al 2013;Mattheis C et al 2013;Mattheis C et al 2012) Light-triggered cytosolic penetration of additives in mammalian cells (Sebai S et al 2010), temperature-triggered biocide activity, samples from 2 to 7 correspond to increasing fraction (from 10 mol% to 54 mol%) of the amino-monomer in the copolymer also shown in Table 1 with transition temperature being in the range 32°C-36°C; (Mattheis C et al 2012) (B) lighttriggered penetration of peptides in cell. Other possible stimuli include temperature or pH.…”

Stimuli‐Induced Core–Corona Inversion of Micelle of Poly(acrylic acid)‐block‐Poly(N‐isopropylacrylamide) and Its Application in Drug Delivery

“…[ 8 ] PNIPAM-functionalized nanostructures possess temperature switched bioadhesive properties. [ 9 ] There are very few reports on the application of schizophrenic micelles even though lots of works have been reported to study the physiological properties of such micelles. [ 3,10 ] In this paper, we systematically study the schizophrenic behavior of PAA-b -PNIPAM diblock copolymer.…”

“…Owing to its thermoresponsive property, PNIPAM has been investigated to fabricate thermoresponsive micelles or hybrid nanostructures for controlled drug release . PNIPAM‐functionalized nanostructures possess temperature switched bio‐adhesive properties …”

Macromolecular cell surface engineering for accelerated and reversible cellular aggregation

“…the biomaterial, is minute and hence the possible cytotoxic or immune responses are minimised. Current cell aggregation methods via CSR include direct cell membrane biotinylation, [16][17][18][19] covalent crosslinking, 20,21 and polyelectrolyte [22][23][24] or ionic mediated cell aggregation. [25][26][27] The further development of such biomaterial-cellular ensembles at the nanoscale is of paramount importance in order to mimic the mechanical, biochemical and interaction cues that occur in the physiological setting.…”