Surface Modification of Microcporous of Polycaprolactone (PCL) Microcarrier to Improve Microcarrier Biocompatibility

Abstract

Oil/water emulsion solvent evaporation method was employed to fabricate polycaprolactone (PCL) microcarriers. In order to produce porous microcarrier, the method was slightly modified. The porous network channels were generated inside the microcarrier by introducing camphene that was dissolved in the same solvent used to dissolve raw PCL. The evaporation of solvent and sublimination of camphene during the process of emulsion solvent evaporation method, produce solidified porous microcarrier beads. The surface porous microcarriers beads were further modified to make it competent for cell attachment and proliferation. The surface of these microcarriers were modified with UV/O 3 system to introduce functional group and charge on the surface. The treatment followed with the immobilization of halal gelatin on the surface to imorove the biocompatibility of the microcarrier. Porous microcarrier with optimal size distribution was successfully fabricated. The average pore size of 11.74±8.32 µm was obtained with the concentration of 20% (w/v) of camphene. The porous PCL microcarrier was further tailored with gelatin through surface treatment with UV/O 3 system and gelatin immobilization and validation of its compatibility towards mammalian cell was tested with cultivation of green monkyer kidney cell (Vero) in the suspension culture. Vero cells attach and proliferatie well on the gelatin coated porous PCL microcarrier with maximum cell number of 3.90 × 10 5 cells/ml as compared to cell proliferation on UV/O 3 treated porous PCL microcarrier (1.83 × 10 5 cells/ml). The developed microcarrier may be potentially applicable as a cell delivery scaffold for cell tissue culture and tissue engineering application.

Development of In Situ Microfluidic System for Preparation of Controlled Porous Microsphere for Tissue Engineering

“…It has a drastically increased volume in the solid phase after polymerization by evaporation. Therefore, highly porous structures in microspheres can be prepared by the reduced volume of polymer and highly increased volumes of CAMP during solvent evaporation [14,17,18]. CAMP is not only nontoxic and environmentally friendly but also soluble in organic solvents with low viscosity [14,17,18].…”

“…Therefore, highly porous structures in microspheres can be prepared by the reduced volume of polymer and highly increased volumes of CAMP during solvent evaporation [14,17,18]. CAMP is not only nontoxic and environmentally friendly but also soluble in organic solvents with low viscosity [14,17,18]. Owing to these properties, they can be easily adopted as pore-generating materials in in situ microfluidic systems.…”