Surface modification of nanofibrous polyethersulfone scaffolds with fluorapatite nanoparticles toward improved stem cell behavior and osteogenic activity in vitro

Shams, Mehdi; Karimi, Mohammad; Jahangir, Vafa; Mohammadian, Milad; Salimi, Ali

doi:10.1016/j.surfin.2022.102512

Cited by 9 publications

(7 citation statements)

References 60 publications

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“…The incorporation of graphene by SPS could strengthen the stiffness of samples due to the crystallization at grain boundaries and the filling of pores in the original structure. In addition, the graphene has a large specific surface area and there are some wrinkles and ripples on the surface of graphene, which could sustain lateral stresses that might affect cytoskeletal tension. , This leads to changes in cytoskeletal organization/structure and induces cell differentiation . Obtained by SPS, composite particles have a strong noncovalent binding capacity and act as preconcentrators for chemicals, resulting in proteins and growth factors accumulate on their surface for osteogenic inducers, accelerating the differentiation progression of MSCs to the osteogenic lineage .…”

Section: Resultsmentioning

confidence: 99%

“…62,63 This leads to changes in cytoskeletal organization/structure and induces cell differentiation. 64 Obtained by SPS, composite particles have a strong noncovalent binding capacity and act as preconcentrators for chemicals, resulting in proteins and growth factors accumulate on their surface for osteogenic inducers, accelerating the differentiation progression of MSCs to the osteogenic lineage. 57 The surface of GNPs flakes in the particles obtained by ultrasound treatment was mostly covered by ECPP particles and therefore could not absorb the molecules directly.…”

Section: Effect Of Particles On Stem Cell Osteogenic Differentiationmentioning

confidence: 99%

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Cascade Regulation of the Proliferation, Recruitment, and Differentiation of Stem Cells to Prevent Aseptic Loosening by GNPs/ECPP Particles Responding to Macrophages: In Vitro and In Vivo

Lei

et al. 2023

ACS Biomater. Sci. Eng.

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Modulating both inflammation and stem cells by designing an artificial joint material to obtain the continuous prevention and control on aseptic loosening (AL) is a novel strategy. In this paper, graphene/europium-doped calcium polyphosphate (GNPs/ECPP) particles were obtained by ultrasound method and spark plasma sintering (SPS) method. The prepared particles were used to modulate the inflammatory response and further obtain cascade regulation on the proliferation, recruitment, and differentiation of stem cells. The results showed that particles obtained by SPS had a stronger effect on promoting the proliferation and differentiation of stem cells, while by ultrasound method more stem cells were recruited. Besides, the graphene and Eu3+ contained in the particles obtained by SPS method could effectively play a synergistic role on the differentiation of stem cells. In vivo experiment results demonstrated that the composite particles effectively suppress the inflammatory response, recruit stem cells, and prevent AL by inhibiting the secretion of inflammatory factors.

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Section: Resultsmentioning

confidence: 99%

Section: Effect Of Particles On Stem Cell Osteogenic Differentiationmentioning

confidence: 99%

Cascade Regulation of the Proliferation, Recruitment, and Differentiation of Stem Cells to Prevent Aseptic Loosening by GNPs/ECPP Particles Responding to Macrophages: In Vitro and In Vivo

Lei

et al. 2023

ACS Biomater. Sci. Eng.

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“…In addition, polymer degradation is dependent on the surface properties of the polymer. Hydrolytic degradation does not occur until water enters the scaffold [ 86 , 87 , 88 ].…”

Section: Resultsmentioning

confidence: 99%

Scaffolds for Cartilage Tissue Engineering from a Blend of Polyethersulfone and Polyurethane Polymers

et al. 2023

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In recent years, one of the main goals of cartilage tissue engineering has been to find appropriate scaffolds for hyaline cartilage regeneration, which could serve as a matrix for chondrocytes or stem cell cultures. The study presents three types of scaffolds obtained from a blend of polyethersulfone (PES) and polyurethane (PUR) by a combination of wet-phase inversion and salt-leaching methods. The nonwovens made of gelatin and sodium chloride (NaCl) were used as precursors of macropores. Thus, obtained membranes were characterized by a suitable structure. The top layers were perforated, with pores over 20 µm, which allows cells to enter the membrane. The use of a nonwoven made it possible to develop a three-dimensional network of interconnected macropores that is required for cell activity and mobility. Examination of wettability (contact angle, swelling ratio) showed a hydrophilic nature of scaffolds. The mechanical test showed that the scaffolds were suitable for knee joint applications (stress above 10 MPa). Next, the scaffolds underwent a degradation study in simulated body fluid (SBF). Weight loss after four weeks and changes in structure were assessed using scanning electron microscopy (SEM) and MeMoExplorer Software, a program that estimates the size of pores. The porosity measurements after degradation confirmed an increase in pore size, as expected. Hydrolysis was confirmed by Fourier-transform infrared spectroscopy (FT-IR) analysis, where the disappearance of ester bonds at about 1730 cm −1 wavelength is noticeable after degradation. The obtained results showed that the scaffolds meet the requirements for cartilage tissue engineering membranes and should undergo further testing on an animal model.

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“…Bioactive glass and calcium phosphates have been used in bone tissue engineering [20]. A synthetic polymer polyethersulfone-based scaffold was recently developed for the growth of MSCs and osteogenic differentiation [21].…”

Section: Introductionmentioning

confidence: 99%

“…Synthetic polymers have the advantage of being mechanically, physically and chemically suited to develop scaffolds [16]. They are easy to manufacture, can be produced on a large scale and can be modi ed according to their applications [6, 21,22]. The major drawback of synthetic polymers is the release of acids like lactic acid, toxic to cell function causing in ammation [4].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic of mesenchymal stem cells growing on RGD conjugated cotton scaffold confers anti-aging, anti-inflammatory and osteogenesis potentials

Aouabdi,

Nedjadi,

Alsiary

et al. 2023

Preprint

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Background Stem cell therapy represents a good alternative therapy to the degenerated or damaged tissue. Stem cells have been used solely or in combination with a scaffold. The latter offers a structural advantage as it allows the cells to grow in 3 Dimensional (3-D) conditions mimicking the in vivo environments. We have previously reported the development of a 3-D Arginyl-glycyl-aspartic acid (RGD)-conjugated cotton-based scaffold for the growth and proliferation of mesenchymal stem cells (MSCs). The scaffold allowed the adhesion of the MSCs and their proliferation without compromising their viability and their stem cell markers. In depth analysis of the molecular changes in the MSCs adhering to the cotton fibers will result in better therapy. Our work aims at identifying the molecular events happening in growing MSCs on the cotton-RGD conjugated-based scaffold through their gene expression profiles. In this study we have seeded MSCs with and without scaffold for 48 hours. Celle were collected for RNA, cDNA synthesis and whole transcriptomic analysis run on both populations. Results Our analysis revealed several up-regulated and down-regulated differently expressed genes in the MSCs adhering to the scaffold compared to the control cells. Gene ontology analysis identified enriched biological processes, molecular functions, pathway and protein-protein interaction in up-regulated and down-regulated genes. Conclusions Our data suggest that the scaffold might have enhanced potential to induce osteogenesis in the MSCs. The results also suggest that the scaffold does not induce oxidative stress, inflammation and aging of the MSCs. Our findings provide useful data for the MSCs applications in tissue engineering and regenerative medicine.

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Surface modification of nanofibrous polyethersulfone scaffolds with fluorapatite nanoparticles toward improved stem cell behavior and osteogenic activity in vitro

Cited by 9 publications

References 60 publications

Cascade Regulation of the Proliferation, Recruitment, and Differentiation of Stem Cells to Prevent Aseptic Loosening by GNPs/ECPP Particles Responding to Macrophages: In Vitro and In Vivo

Cascade Regulation of the Proliferation, Recruitment, and Differentiation of Stem Cells to Prevent Aseptic Loosening by GNPs/ECPP Particles Responding to Macrophages: In Vitro and In Vivo

Scaffolds for Cartilage Tissue Engineering from a Blend of Polyethersulfone and Polyurethane Polymers

Transcriptomic of mesenchymal stem cells growing on RGD conjugated cotton scaffold confers anti-aging, anti-inflammatory and osteogenesis potentials

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