Surface modification of parylene-N films for the culture of osteoblast-like cells (MG-63)

Liaqat, Usman; Ko, Hyun-Yoon; Suh, Hwal; Lee, Misu; Pyun, Jae Chul

doi:10.1016/j.apsusc.2016.03.218

Cited by 15 publications

(25 citation statements)

References 29 publications

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“…The surface of UV-treated parylene N film contains several types of oxygen-bearing functional groups that increase its hydrophilicity 4 . The UV-treated parylene N was used for the comparison with other surfaces because this surface showed a significant influence on the cell proliferation of osteoblast-and neuron-like cells as well as the cell differentiation of neuron-like cells in the previous work 5,6 . As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, the functional groups on the parylene film surface can be modified by ultraviolet (UV) light or plasma treatments 4 . Surface-modified parylene provides different microenvironments for proliferation, as well as differentiation of osteoblast-and neuron-like cells 5,6 . For instance, for both proliferation and differentiation of PC12 cells, negatively charged oxygen-bearing surface functional groups are required for the stable attachment of dendrite-like structures 5 .…”

Section: Modified Parylene-n Films As Chemical Microenvironments For mentioning

confidence: 99%

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Modified parylene-N films as chemical microenvironments for differentiation and spheroid formation of osteoblast cells

Kim

Lee

et al. 2020

Sci Rep

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In this work, the influence of parylene N film on the spheroid formation of osteoblast-like cells (MG-63) was determined and compared with that of high-hydrophilicity microenvironments, such as hydrophilic culture matrix and ultraviolet-treated parylene N film. To elucidate the change in cell properties due to the microenvironment of parylene N film, global gene expression profiles of MG-63 cells on parylene N film were analyzed. We confirmed the upregulated expression of osteoblast differentiation- and proliferation-related genes, such as Runx2, ALPL, and BGLAP and MKi67 and PCNA, respectively, using the real-time polymerase chain reaction. In addition, the differentiation and proliferation of osteoblast cells cultured on parylene N film were validated using immunostaining. Finally, the formation of spheroids and regulation of differentiation in human mesenchymal stem cells (MSCs) on parylene N film was demonstrated. The results of this study confirm that the microenvironment with the controlled hydrophobic property of parylene N film could effectively trigger the bone differentiation and maintains the proliferation of MSCs, similar to MG-63 cells without any scaffold structures or physical treatments.

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Section: Resultsmentioning

confidence: 99%

Section: Modified Parylene-n Films As Chemical Microenvironments For mentioning

confidence: 99%

Modified parylene-N films as chemical microenvironments for differentiation and spheroid formation of osteoblast cells

Kim

Lee

et al. 2020

Sci Rep

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“…To achieve the required mechanical properties of polymers, different blends and composites are prepared, and surface modification is performed to achieve the desired surface and biological properties. Numerous hybrid composite biomaterials or scaffolds prepared from combinations of either natural or synthetic materials have been investigated to obtain the synergistic properties required for bone tissue engineering [ 3 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation of PBS/PLLA/HAP Composites by the Solution Casting Method: Mechanical Properties and Biocompatibility

et al. 2020

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The use of biodegradable polymeric scaffolds for tissue regeneration is becoming a common practice in the clinic. Therefore, an inclined trend is developing with regards to improving the mechanical properties of these scaffolds. Here, we aim to improve the mechanical properties of poly (butylene succinate) (PBS)/poly (l-lactic acid) (PLLA) blends by incorporating hydroxyapatite nanoparticles (HAP) in the blends to form composites. PBS/PLLA = 100/0, 95/5, 90/10, 85/15, and 0/100 wt% blends, along-with the loadings of a few mg of HAPs, were prepared using the solution casting method. A scanning electron microscope showed the voids and droplets, indicating the immiscibility of blends. Due to this immiscibility, the tensile strength values of the blends were found to be in between that of pure PBS (42.85 MPa) and pure PLLA (31.39 MPa). HAPs act as a compatibilizer by incorporating themselves in the voids and spaces caused by the immiscibility, thus increasing the overall tensile strength of the resulting composite to a certain extent, e.g., the tensile strength of PBS/PLLA = 95/5 loaded with 50 mg HAPs was found to be 51.16 MPa. The structural analysis employing the X-ray diffraction (XRD) patterns confirmed the formation of polymer blends and composites. The contact angle analysis showed that the addition of HAPs increased the hydrophilicity of the resulting composites. Selective samples were investigated based on mechanical properties to see if the blends and composites are biocompatible. The obtained results showed that all of the samples with better mechanical properties demonstrated good biocompatibility. This indicates the effectiveness of scaffolds for tissue regeneration.

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“…amine groups on its phenyl group-containing backbone (p-xylylene), was confirmed [32]. When the AP membranes were treated with glutaraldehyde, the amine peak disappeared and new C-H stretching peaks corresponding to the aliphatic groups appeared at 2919 cm −1 and to the aldehyde groups appeared at 2850 cm − 1 (Fig.…”

Section: Fabrication Of the Urease Immobilized Ptfe Membranes Using Amentioning

confidence: 80%

Parylene-Coated PTFE-Membrane-Based Portable Urea Sensor for Use in Fast Flow Conditions

Park¹,

Kim²,

Kim³

et al. 2019

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A portable urea sensor for use in the fast flow condition was fabricated using porous polytetrafluoroethylene (PTFE) membranes coated with amine-functionalized parylene, parylene-A, by vapor deposition. To generate a specific electrochemical sensor signal from urea, the urea-hydrolyzing enzyme urease was immobilized on the parylene-A-coated PTFE membranes via chemical crosslinking using glutaraldehyde. The urease-immobilized membranes were assembled in a polydimethylsiloxane (PDMS) fluidic chamber, and a screen-printed carbon three-electrode system was used for electrochemical measurements. The success of urease immobilization was confirmed using fluorescence microscopy, scanning electron microscopy, and Fourier-transform infrared spectroscopy. The optimum concentration of urease for immobilization on the parylene-A-coated PTFE membranes was determined to be 48 mg/mL, and the optimum number of membranes in the PDMS chamber was found to be 8. Using these optimized conditions, we fabricated the urea biosensor and monitored urea samples under various flow rates ranging from 0.5 to 10 mL/min in the flow condition using chronoamperometry. To test the applicability of the sensor for physiological samples, we used it for monitoring urea concentration in the waste peritoneal dialysate of a patient with chronic renal failure, at a flow rate of 0.5 mL/min.

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Surface modification of parylene-N films for the culture of osteoblast-like cells (MG-63)

Cited by 15 publications

References 29 publications

Modified parylene-N films as chemical microenvironments for differentiation and spheroid formation of osteoblast cells

Modified parylene-N films as chemical microenvironments for differentiation and spheroid formation of osteoblast cells

Preparation of PBS/PLLA/HAP Composites by the Solution Casting Method: Mechanical Properties and Biocompatibility

Parylene-Coated PTFE-Membrane-Based Portable Urea Sensor for Use in Fast Flow Conditions

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