Surface modification of PLGA microspheres

Abstract

Microspheres made of poly(lactic‐co‐glycolic acid) (PLGA) are biocompatible and biodegradable, rendering them a promising tool in the context of drug delivery. However, nonspecific adsorption of plasma proteins on PLGA micro‐ and nanospheres is a main limitation of drug targeting. Poly(L‐lysine)‐g‐poly(ethylene glycol) (PLL‐g‐PEG), physisorbed on flat metal oxide surfaces, has previously been shown to suppress protein adsorption drastically. The goal of our work was to characterize the efficiency of the protein repellent character of PLL‐g‐PEG on PLGA microspheres and to show the feasibility of introducing functional groups on the PLGA microspheres via functionalized PLL‐g‐PEG. To quantify the adsorbed amount of protein, a semiquantitative method that uses confocal laser scanning microscopy (CLSM) was applied. The first part of the experiment confirms the feasibility of introducing specific functional groups on PLL‐g‐PEG–coated PLGA microspheres. In the second part of the experiment, PLL‐g‐PEG–coated PLGA microspheres show a drastic decrease of adsorbed proteins by two orders of magnitude in comparison to uncoated PLGA microspheres. Low protein‐binding, functionalizable microspheres provide a fundamental basis for the design of drug delivery and biosensor systems. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 66A: 55–61, 2003

Modeling the Time Course of the Tissue Responses to Intramuscular Long-acting Paliperidone Palmitate Nano-/Microcrystals and Polystyrene Microspheres in the Rat

“…Conversely, the adsorbed protein layer is a reflection of the material's surface properties and is generally known to be greatly influenced thereby (Müller et al 2003). Hence, the nano-/microparticle surface properties likely modulate the progress of the overall host response.…”

Understanding the adsorption mechanism of chitosan onto poly(lactide-co-glycolide) particles

“…Cetyl trimethyl ammonium bromide (CTAB), as a cationic surfactant, has been applied to coat PLGA cationic particles for efficient DNA vaccine delivery [8,9]. Cationic polymers, including polyethyleneimine (PEI) [10][11][12], polylysine [13], chitosan [14,15], and gelatin [16], have also been reported as coating materials for biodegradable PLGA or poly (D,L-lactic acid) particles [17] as gene carriers. Cationic surface modification of nanoparticles can be obtained either during the formation of PLGA particles or by incubating polyelectrolytes with preformed PLGA particles [16], using emulsion-solvent evaporation or spray-drying techniques.…”

PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays

“…Decreased NSA of proteins on PEGylated versus neat surfaces has been reported in several reports (see, e.g., references 35,[53][54][55][56] ). An antifouling effect of PEG was observed also in this study, where the NSA of BSA on neat PGMA-COOH, CH 3 -PEG 30,000 -NH 2 -PGMA, and CH 3 -PEG 2,000 -NH 2 -PGMA microspheres was compared (Fig.…”

“…Consequently, a rapid and simple method to improve particle surface properties is required. PEG was chosen for its antifouling properties, which have been so far deeply investigated in a relation with proteins, cells, DNA [53][54][55]58 etc.…”