Surface modification with thermoresponsive polymer brushes for a switchable electrochemical sensor

Comminges, Clément; Frasca, Stefano; Sütterlin, Martin; Wischerhoff, Erik; Laschewsky, André; Wollenberger, Ulla

doi:10.1039/c4ra07190e

Cited by 9 publications

(5 citation statements)

References 30 publications

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“…Thermoresponsive hydrogels that can spontaneously gel and/or undergo swelling/deswelling transitions upon heating , have attracted considerable recent attention in applications including drug delivery, , tunable cell adhesion, , switchable biosensors with on/off potential, , reversible biomolecule scavenging, , and microfluidic gates. , Lower critical solution temperature (LCST) polymer-based hydrogels are by far the most commonly applied type of thermoresponsive hydrogel, ,, with poly( N -isopropylacrylamide) (PNIPAM) being the most widely investigated. In the context of cell engineering, PNIPAM-based structures have shown particular promise for thermoreversible cell adhesion and recovery, with Okano’s lab (using PNIPAM-grafted glass surfaces fabricated by surface-initiated reversible addition–fragmentation chain transfer (SI-RAFT) polymerization) and Takezawa’s lab (using PNIPAM/collagen coatings on polystyrene) both reporting such functionality as far back as 1990; − cells grown into coherent cell sheets could adhere to the substrate at 37 °C but delaminated from the substrate at 20 °C for 1 h .…”

Section: Introductionmentioning

confidence: 99%

“…Thermoresponsive hydrogels that can spontaneously gel and/ or undergo swelling/deswelling transitions upon heating 1,2 have attracted considerable recent attention in applications including drug delivery, 3,4 tunable cell adhesion, 5,6 switchable biosensors with on/off potential, 7,8 reversible biomolecule scavenging, 9,10 and microfluidic gates. 11,12 Lower critical solution temperature (LCST) polymer-based hydrogels are by far the most commonly applied type of thermoresponsive hydrogel, 1,13,14 with poly(N-isopropylacrylamide) (PNIPAM) being the most widely investigated.…”

Section: ■ Introductionmentioning

confidence: 99%

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Fast Thermoresponsive Poly(oligoethylene glycol methacrylate) (POEGMA)-Based Nanostructured Hydrogels for Reversible Tuning of Cell Interactions

Lam

Pan

et al. 2021

ACS Biomater. Sci. Eng.

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Reactive electrospinning is demonstrated as a viable method to create fast-responsive and degradable macroporous thermoresponsive hydrogels based on poly(oligoethylene glycol methacrylate) (POEGMA). Hydrazide-and aldehyde-functionalized POEGMA precursor polymers were coelectrospun to create hydrazone cross-linked nanostructured hydrogels in a single processing step that avoids the need for porogens, phase separation-driving additives, or scaffold postprocessing. The resulting nanostructured hydrogels can respond reversibly and repeatedly to changes in external temperature within seconds, in contrast to the minutes-tohours response time observed with bulk hydrogels. Furthermore, nearly quantitative cell delamination can be achieved within 2 min of incubation at 4 °C, resulting in the recovery of as many or more (as well as more proliferatively active) cells from the substrate relative to the conventional trypsinization protocol. The combined macroporosity, nanoscale feature size, and interfacial switching potential of these nanostructured hydrogels thus offer promise for manipulating cell−hydrogel interactions as well as other applications in which rapid responses to external stimuli are desirable.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Fast Thermoresponsive Poly(oligoethylene glycol methacrylate) (POEGMA)-Based Nanostructured Hydrogels for Reversible Tuning of Cell Interactions

Lam

Pan

et al. 2021

ACS Biomater. Sci. Eng.

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“…Therefore further investigations of the influence of the coupling procedure, size and nature of the nanoparticles are needed to understand and apply the nanoparticles in bioelectrocatalytic devices. Dithiobis(succinimidyl propionate) DTSP is an SAM‐forming crosslinking agent that allows covalent attachment of macromolecular surface amino groups for example of antibodies , polyelectrolytes and nanoparticle assemblies .…”

Section: Introductionmentioning

confidence: 99%

Role of Conductive Nanoparticles in the Direct Unmediated Bioelectrocatalysis of Immobilized Sulfite Oxidase

et al. 2016

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1Introduction Molybdenum (Mo) containing enzymes have been associated with the catalysis of important reactions in the metabolism of smallm oleculess uch as carbon nitrogen, and sulfur [1a, b].T he sulfite oxidizing enzymes sulfite oxidase (SO) and sulfite dehydrogenase (SDH) catalyze the oxidation of sulfite to sulfate accompaniedw ith the release of two electrons and two protons according to the following equation:All sulfite oxidizing enzymes share the same catalytic Mo cofactor (Moco) containingu nit. But the number and type of additional redoxc enters differb etween enzymes from different classes of organisms, which has an impact on the type of electrona cceptor the enzyme can use [2a, b].O nly in the vertebrate SO ah eme b binding domaini sc ovalently linkedt ot he core structure of the enzyme.T he human sulfite oxidase (hSO) shows al arge structural homology to the enzyme from chicken liver (cSO) which is ad imeric enzyme where each subunit of the homodimer consists of an N-terminal heme b binding domain( SO-HD), followed by ap olypeptide tetherc onnectingt he heme domain to the Moco-containing domain (SO-MD) and ad imerization domain at the C-terminus [3].Thes ingle steps of the SO catalyzed reaction involve the two-electron oxidationo fs ulfite to sulfate at the Moco center, where Mo VI is reduced to Mo IV .I nv ertebrate SO the Mo VI regeneration is naturally coupled to the reduction of two equivalents of soluble cytochrome c which proceeds in two subsequent intra-and intermolecular singlee lectron transfer steps via the heme b5 containing site [4a, b, c].T hese steps involve ar epositioning of the domains to reduce electron transfer distances permitting electron transfer from Mo to Fe of SO-HD and to cytochrome c [5a, b].Areaction with soluble [6a, b] and polymerb ound redoxm ediators is also possible [7a, b]. Direct unmediated electron transfer from the reduced enzyme to electrodes proceedsv ia the hemed omain [8]. This process was most effective when the enzymei si mmobilized on electrode surfaces modified with positive charged SAMs facilitating the oriented assembly of SO electron transfer and bioelectrocatalysis [9a, b].Abstract:W er eport efficient bioelectrocatalytic sulfite oxidation by human sulfite oxidase (hSO) immobilizedo n ag old nanoparticle (AuNP)m odified gold electrode.T he AuNP were synthesized in aqueous phase by using branched polyethyleneimine (PEI) as reducing as well as stabilizing agent. Golde lectrodes were modified by as elf assembled monolayer of dithio-bis(N-hydroxysuccinimidyl propionate) (DTSP) onto which the NP and hSO were immobilized. Cyclic voltammetry of the hSO modified electrode in the absence of substrater evealedaquasi-reversible direct electrochemical reactiono ft he heme domaino fhSO with fast electron transfer rate.T he electron transferr ate constant of k s = 32 s À1 and the formal potential E 0 ' = À0.155 Vv s. Ag/AgCl/1 MK Cl were estimated. Comparatives tudies with nanoparticles of BaSO 4 indicate the importance of the NP conductivity for charge transfer an...

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“…Them otivation driving this research is based on possible applications of the thermo-sensitive modified electrodes and electrochemicals ystems in variousb ioelectronic and biosensor devices responding to externals ignals such as temperature variation. Thermo-switchable electrochemical sensors [75] and immune-sensors [ 76],m olecular-release systems [ 77] and bioelectrocatalytic systems [78] have been designedb ased on the temperature-sensitive polymers.…”

Section: Discussionmentioning

confidence: 99%

Modified Electrodes and Electrochemical Systems Switchable by Temperature Changes

Katz

2016

Electroanalysis

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This article is an overview of extensive research efforts in the area of temperature‐controlled electrochemical systems. Electrochemical reactions, including electrocatalytic and bioelectrocatalytic processes, have been reversibly activated and inhibited by temperature changes. This was achieved by modification of electrode surfaces with thermo‐sensitive polymers (e.g., poly(N‐isopropylacrylamide), PNIPAM) which are reversibly switched by temperature changes between two different structures: swollen expanded coil conformation and shrunken collapsed globule state. While the swollen hydrophilic state allows penetration of redox species to the electrode conducting support and activates electrochemical reactions, the collapsed hydrophobic state isolates the electrode surface and inhibits electrochemical processes. Electrodes modified with the thermo‐switchable polymers have been additionally functionalized with photo‐switchable molecules (e.g., spiropyran derivatives) to achieve double‐controlled electrochemical reactions switchable by temperature changes and light signals. Incorporation of metallic nanoparticles or graphene species in the temperature‐sensitive polymer films resulted in sophisticated features and multi‐signal controlled behavior of the nano‐composite systems.

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Surface modification with thermoresponsive polymer brushes for a switchable electrochemical sensor

Cited by 9 publications

References 30 publications

Fast Thermoresponsive Poly(oligoethylene glycol methacrylate) (POEGMA)-Based Nanostructured Hydrogels for Reversible Tuning of Cell Interactions

Fast Thermoresponsive Poly(oligoethylene glycol methacrylate) (POEGMA)-Based Nanostructured Hydrogels for Reversible Tuning of Cell Interactions

Role of Conductive Nanoparticles in the Direct Unmediated Bioelectrocatalysis of Immobilized Sulfite Oxidase

Modified Electrodes and Electrochemical Systems Switchable by Temperature Changes

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