Surface PEGylation of Mesoporous Silica Nanorods (MSNR): Effect on loading, release, and delivery of mitoxantrone in hypoxic cancer cells

Wani, Amit; Savithra, Galbokka H. Layan; Abyad, Abdulrazak; Kanvinde, Shrey; Li, Jing; Brock, Stephanie L.; Oupický, David

doi:10.1038/s41598-017-02531-4

Cited by 43 publications

(27 citation statements)

References 48 publications

(66 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…27 On the other hand, IC50 values for mesoporous silica nanorods loaded with MTO vs. free MTO against triple negative breast cancer cells MDA-MB-231 lied at 548 and 966 nM, respectively. 23 The latter is in good agreement with our studies. We therefore chose the triple negative model for investigation of drug efficacy using the TMV delivery approach in-vivo .…”

Section: Resultssupporting

confidence: 93%

See 1 more Smart Citation

Tobacco mosaic virus delivery of mitoxantrone for cancer therapy

Lin

Steinmetz

2018

Nanoscale

View full text Add to dashboard Cite

Mitoxantrone (MTO) is a topoisomerase II inhibitor which has been used to treat various forms of cancer either as a solo chemotherapy regimen or as a component in cocktail treatments. However, as with other anti-neoplastic agents, MTO has severe cardiac side effects. Therefore, a drug delivery approach holds promise to improve the safety and applicability of this chemotherapy. Here, we report the application of a plant virus-based nanotechnology derived from tobacco mosaic virus (TMV) as a delivery vehicle for MTO towards cancer therapy. TMV is a high aspect-ratio, soft-matter nanotube with dimensions of 300 × 18 nm and a 4 nm wide channel. The surface chemistry of the interior and exterior TMV surfaces is distinct and we established charge-driven drug loading strategies to encapsulate therapeutics for drug delivery. We demonstrate effective MTO loading into TMV yielding ∼1000 MTO per TMV carrier. The treatment efficacy of MTO-loaded TMV (MTOTMV) was assessed in in vitro and in vivo models. In vitro testing confirmed that MTO maintained its efficacy when delivered by TMV in a panel of cancer cell lines. Drug delivery in vivo using a mouse model of triple negative breast cancer demonstrated the superior efficacy of TMV-delivered MTO vs. free MTO. This study demonstrates the potential of plant virus-based nanotechnology for cancer therapy and drug delivery.

show abstract

Section: Resultssupporting

confidence: 93%

“…mesoporous silica nanorods. 23 Increased stability under physiologic conditions, e.g. during circulation (pH 7.4), and increased drug release at lower pH (pH 5.0) is expected and desired: under acidic conditions, the carboxylic acid will be protonated thus weakening the charge interactions with MTO triggering its release.…”

Section: Resultsmentioning

confidence: 99%

Tobacco mosaic virus delivery of mitoxantrone for cancer therapy

Lin

Steinmetz

2018

Nanoscale

View full text Add to dashboard Cite

show abstract

“…Zeta potential measurements were also performed, and the data at pH 6.5 for Si particles modified with PEG ( Figure a) showed a significant shift of surface charge from −36 to −3 mV for Si5 and from −45 to −5.5 mV for Si2, indicating successful surface immobilization of PEG on the Si particles. The finding that the surface charge approaches neutral after PEG grafting has been reported in earlier work . When comparing both types of Si–PEG particles, the Si5–PEG showed a more positive shift (−3 ± 1.7 mV) compared with Si2–PEG (−5.5 ± 2.3 mV) indicating that the Si5 particles had a greater number of bound PEG molecules.…”

Section: Resultssupporting

confidence: 83%

Tunable Chemical and Topographic Patterns Based on Binary Colloidal Crystals (BCCs) to Modulate MG63 Cell Growth

Diba

Reynolds

Thissen

et al. 2019

Adv Funct Materials

View full text Add to dashboard Cite

More effective and diversified surface modification strategies are required for materials used in biomedical engineering. Combining surface modification involving bioactive signals or nonfouling polymers with tunable topography has the potential to meet this need. Here, a method is reported to generate bioactive surfaces having tunable topographies based on self-assembled binary colloidal crystals (BCCs), where the colloids are premodified with nonfouling molecules or cell adhesive peptides. The BCCs are fabricated from silica (Si) microspheres and polymer nanospheres. The Si microspheres are either modified with poly(ethylene glycol) (PEG) or with the cell adhesive arginine-glycine-aspartic acid (RGD) peptide prior to BCC fabrication. Four types of BCCs are explored in cell studies using MG63 cells. BCC surfaces coupled with PEG or RGD peptides are found to significantly modulate cell adhesion, spreading, and morphology, which is attributed to the combination of BCC topography and the molecules at the particle surface within the BCC. PEG-modified BCCs are expected to find applications where limited cell adhesion is required, while the RGD-modified BCCs have the potential for enhancing cell growth on medical devices such as bone implants. More advanced cell biology applications such as controlled stem cell differentiation are also anticipated to find use from BCCs.

show abstract

“…Despite no therapeutic effect, the data corroborate the safety of the magnetic core mesoporous silica nanoparticles doped with trastuzumab and labelled with 99mTc to be used as an imaging drug. Also, the data obtained from the MTT's test to the pure magnetic core mesoporous silica nanoparticles are consistent with the literature findings, corroborating its safety [24][25][26]. It is important to notice a slight difference between the magnetic core mesoporous silica loaded with trastuzumab and the pure magnetic core mesoporous silica was found, this difference is due to the effect of trastuzumab on the cell viability.…”

Section: Cytotoxicity Assaysupporting

confidence: 88%