Surface Plasmon Resonance Detection for Capillary Electrophoresis Separations

Abstract

A miniaturized surface plasmon resonance sensor has been used as an on-line detector for capillary electrophoresis separations. The capillary was modified slightly to shield the sensor electronics from the high voltages applied during the separation. A three-component mixture of high refractive index materials was separated and detected at the millimolar level by an untreated gold-sensing surface. A simple protein immobilization procedure was used to functionalize the surface for selective protein detection. A hybrid buffer system was developed, in which both the deposition of immobilized protein layers and the electrophoretic delivery of protein analytes were optimized. The detection system has a reproducibility of 15%, a dynamic range of 3 orders of magnitude, and a detection limit for IgG of 2 fmol.

In situ sensing of metal ion adsorption to a thiolated surface using surface plasmon resonance spectroscopy

“…Surface plasmon resonance (SPR) spectroscopy is a powerful tool for the in situ real-time characterization of a solid/liquid interface [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. Surface plasmons are collective oscillations of free electrons at an interface between a thin metal film and a dielectric medium.…”

Quantitative multispectral biosensing and 1D imaging using quasi-3D plasmonic crystals

“…Such systems can be probed by immobilizing one component of a binding pair on a surface and following the mass coverage change upon exposure to the complementary recognition element (6)(7)(8)(9). Surface plasmon resonance (SPR) instruments that measure changes in refractive index near a metal surface accomplish this aim with high sensitivities, without the need for fluorescent labels (10,11). Conventional SPR systems use prisms to couple light into a single surface plasmon mode on a flat, continuous metal (typically gold) film (12).…”

Portable capillary-based (non-chip) capillary electrophoresis

“…Other types of detection cells have been based on the optical properties of the analyte (e.g., an integrated refractive-index, optical-ring-resonator detector was presented in the work of Zhu et al in 2007 [133]). Miniaturized surface plasmon resonance detection for CE was presented by Whelan et al [134] in 2003.…”