Surface plasmon resonance detection of small molecule using split aptamer fragments

Femtomolar and selective dopamine detection by a gold nanoparticle enhanced surface plasmon resonance aptasensor

“…Zhou et al reported the use of gold nanoparticles-aptamer conjugates as a competitive reagent to detect adenosine down to 0.1 nM [33]. Split aptamer together with gold nanoparticle enhancement was utilized to detect adenosine with a limit of detection of 1.5 pM [34]. Based on these work, we believe a nanoparticleenhanced SPR assay combined with highly specific recognition element could push the detection limit for dopamine to lower pM or fM, thus close the gap mentioned above.…”

Surface plasmon resonance biosensor for enzyme-free amplified microRNA detection based on gold nanoparticles and DNA supersandwich

“…On the one hand, the SPR signal was enhanced by the electronic coupling between localized plasmon of AuNPs and the surface plasmon wave associated with Au film [20,21]. On the other hand, AuNPs as the primary amplification element, could also initiate the formation of DNA supersandwich, i.e.…”

“…The principle of this novel assay for miRNA-21 detection was shown in A c c e p t e d M a n u s c r i p t 6 DNA-linked AuNPs were captured on the surface of Au film by hybridizing with the terminus of capture DNA. Due to the electronic coupling between the localized plasmon of AuNPs and the surface plasmon wave associated with Au film, the shift of resonance angle was enhanced [20,21]. Then, as report DNA1 and report DNA2 were introduced, DNA-linked AuNPs initiated the formation of DNA supersandwich structure.…”

“…In this work, microRNA-21 (miRNA-21), which had high expression in breast, ovarian, diffuse large B-cell lymphoma and myocardial diseases [24,25], was used as a model. The principle of this novel assay for miRNA-21 detection was shown in [20,21]. Then, as report DNA1 and report DNA2 were introduced, DNA-linked AuNPs initiated the formation of DNA supersandwich structure.…”

Biosensors Based on Nanoparticles and Electrochemical Detection

“…Concerning the biofunctionalization process, several proteins, antibodies, antigens, and nucleic acids are used in order to make them viable for biomedical, environmental, or food sensing. [15][16][17][18][19] Biosensors could be classified into electrochemical, mass-sensitive, and optical sensors depending on the signal's transduction ( Figure 10.1):…”