Surface Plasmon Resonance Microscopy: From Single‐Molecule Sensing to Single‐Cell Imaging

Zhou, Xinglu; Yang, Yuanyuan; Wang, Shaopeng; Liu, Xian‐Wei

doi:10.1002/anie.201908806

Cited by 130 publications

(106 citation statements)

References 73 publications

Supporting

Mentioning

104

Contrasting

Unclassified

Order By: Relevance

“…Scattering of the plasmonic waves by individual bacterial cells on the surface generated interferometric single-cell images with high contrast, which were recorded by a charge-coupled device camera. The plasmonic scattering intensity of the bacterial cell decreases exponentially as the distance between the cell and surface ( 21 , 22 ). This feature enables a sensitive determination of the vertical displacement, Z , of individual bacterial cells.…”

Section: Resultsmentioning

confidence: 99%

Plasmonic probing of the adhesion strength of single microbial cells

Liu

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Probing the binding between a microbe and surface is critical for understanding biofilm formation processes, developing biosensors, and designing biomaterials, but it remains a challenge. Here, we demonstrate a method to measure the interfacial forces of bacteria attached to the surface. We tracked the intrinsic fluctuations of individual bacterial cells using an interferometric plasmonic imaging technique. Unlike the existing methods, this approach determined the potential energy profile and quantified the adhesion strength of single cells by analyzing the fluctuations. This method provides insights into biofilm formation and can also serve as a promising platform for investigating biological entity/surface interactions, such as pathogenicity, microbial cell capture and detection, and antimicrobial interface screening.

show abstract

Section: Resultsmentioning

confidence: 99%

Plasmonic probing of the adhesion strength of single microbial cells

Liu

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The principle of high NA SPRi is illustrated in Fig. 7 a, as the p-polarized monochromatic light passes through the objective and reaches the interface of gold and glass in a total internal reflection (TIR) mode, evanescent waves are produced ( Zhou et al, 2020 ). At the same time, the surface plasmon polaritons (SPPs) also propagate along the interface originated from the free electrons of gold.…”

Section: Direct Visualization For “Digital” Detectionmentioning

confidence: 99%

Section: Direct Visualization For “Digital” Detectionmentioning

confidence: 99%

A critical review: Recent advances in “digital” biomolecule detection with single copy sensitivity

Liu

2021

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…Subtle RI variations adjacent to the interface have large influences on the resonance angle, enabling the applications of the shift in the resonance angle in SPR biosensors. [ 133 ]…”

Section: Advanced Optical Techniques Utilizing Single Plasmonic Nanopmentioning

confidence: 99%

Individual Plasmonic Nanoprobes for Biosensing and Bioimaging: Recent Advances and Perspectives

Wang

Feng

et al. 2021

Small

View full text Add to dashboard Cite

With the advent of nanofabrication techniques, plasmonic nanoparticles (PNPs) have been widely applied in various research fields ranging from photocatalysis to chemical and bio‐sensing. PNPs efficiently convert chemical or physical stimuli in their local environment into optical signals. PNPs also have excellent properties, including good biocompatibility, large surfaces for the attachment of biomolecules, tunable optical properties, strong and stable scattering light, and good conductivity. Thus, single optical biosensors with plasmonic properties enable a broad range of uses of optical imaging techniques in biological sensing and imaging with high spatial and temporal resolution. This work provides a comprehensive overview on the optical properties of single PNPs, the description of five types of commonly used optical imaging techniques, including surface plasmon resonance (SPR) microscopy, surface‐enhanced Raman scattering (SERS) technique, differential interference contrast (DIC) microscopy, total internal reflection scattering (TIRS) microscopy, and dark‐field microscopy (DFM) technique, with an emphasis on their single plasmonic nanoprobes and mechanisms for applications in biological imaging and sensing, as well as the challenges and future trends of these fields.

show abstract

Surface Plasmon Resonance Microscopy: From Single‐Molecule Sensing to Single‐Cell Imaging

Cited by 130 publications

References 73 publications

Plasmonic probing of the adhesion strength of single microbial cells

Plasmonic probing of the adhesion strength of single microbial cells

A critical review: Recent advances in “digital” biomolecule detection with single copy sensitivity

Individual Plasmonic Nanoprobes for Biosensing and Bioimaging: Recent Advances and Perspectives

Contact Info

Product

Resources

About