Surface properties of and cell adhesion onto allylamine-plasma-coated polyethylenterephtalat membranes

Hamerli, P.; Weigel, Th.; Groth, Thomas; Paul, D.

doi:10.1016/s0142-9612(03)00312-0

Cited by 230 publications

(183 citation statements)

References 35 publications

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“…However, this cell adhesive effect of PPAAm is not restricted to osteoblasts, as it also could be confirmed for platelets, HUVEC, fibroblasts (Hamerli et al, 2003;Intranuovo et al, 2011;Yang et al, 2012;Yang et al, 2011), human gingiva epithelial and uroepithelial cells as well as human stem cells in vitro (Hamerli et al, 2003;Intranuovo et al, 2011;Yang et al, 2012;Yang et al, 2011). As expected, CD caused a significant decline in cell area, which underlines the importance of an intact actin cytoskeleton for changes in cell shape during the spreading process.…”

Section: F Kunz Et Alsupporting

confidence: 62%

Osteoblasts with impaired spreading capacity benefit from the positive charges of plasma polymerised allylamine

Kunz

Rebl²,

Quade³

et al. 2015

eCM

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Bone diseases such as osteoporosis, osteoarthritis and rheumatoid arthritis, impinge on the performance of orthopaedic implants by impairing bone regeneration. For this reason, the development of effective surface modifications supporting the ingrowth of implants in morbid bone tissue is essential. Our study is designed to elucidate if cells with restricted cell-function limiting adhesion processes benefit from plasma polymer deposition on titanium. We used the actin filament disrupting agent cytochalasin D (CD) as an experimental model for cells with impaired actin cytoskeleton. Indeed, the cell's capacity to adhere and spread was drastically reduced due to shortened actin filaments and vinculin contacts that were smaller. The coating of titanium with a positively charged nanolayer of plasma polymerised allylamine (PPAAm) abrogated these disadvantages in cell adhesion and the CD-treated osteoblasts were able to spread significantly. Interestingly, PPAAm increased spreading by causing enhanced vinculin number and contact length, but without significantly reorganising actin filaments. PPAAm with the monomer allylamine was deposited in a microwave-excited low-pressure plasma-processing reactor. Cell physiology was monitored by flow cytometry and confocal laser scanning microscopy, and the length and number of actin filaments was quantified by mathematical image processing. We showed that biomaterial surface modification with PPAAm could be beneficial even for osteoblasts with impaired cytoskeleton components. These insights into in vitro conditions may be used for the evaluation of future strategies to design implants for morbid bone tissue.

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Section: F Kunz Et Alsupporting

confidence: 62%

Osteoblasts with impaired spreading capacity benefit from the positive charges of plasma polymerised allylamine

Kunz

Rebl²,

Quade³

et al. 2015

eCM

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“…An example application is given in Figure 7 whereby boron nitride nano-tubes (BNNT) were plasma surface treated to modify the BNNT wettability and adhesion characteristics. On account of its benefits, it has extensively been shown that plasma surface treatment can give rise to an enhanced biological cell response [130][131][132][133][134][135][136][137]. Having said this, Arefi-Khonsari et al [138] showed that certain polymers such as octadecyltrichlorosilane self-assembled monolayers (OTS-SAM) are much more resistant to this technique compared to polyethylene (PE), indicating that the successful implementation of this particular technique is material dependent.…”

Section: Plasma Surface Treatmentmentioning

confidence: 99%

Surface Treatments to Modulate Bioadhesion: A Critical Review

Waugh¹,

Toccaceli²,

Gillett³

et al. 2016

rev adhes adhesives

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On account of the recent increase in importance of biological and microbiological adhesion in industries such as healthcare and food manufacturing many researchers are now turning to the study of materials, wettability and adhesion to develop the technology within these industries further. This is highly significant as the stem cell industry alone, for example, is currently worth £3.5 million in the United Kingdom (UK) alone. This paper reviews the current state-of-the-art techniques used for surface treatment with regards to modulating biological adhesion including laser surface treatment, plasma treatment, micro/nano printing and lithography, specifically highlighting areas of interest for further consideration by the scientific community. What is more, this review discusses the advantages and disadvantages of the current techniques enabling the assessment of the most attractive means for modulating biological adhesion, taking in to account cost effectiveness, complexity of equipment and capabilities for processing and analysis.

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“…Amine plasma treatment causes a decrease in WCA due to the incorporation of hydrophilic nitrogen and oxygen moieties [43][44][45] onto the sample surface. Oxygen functionalities can be incorporated via two different processes.…”

Section: Water Contact Angle Measurements (Wca)mentioning

confidence: 99%

“…Since a wide range of chemical functionalities is introduced on the surface of a plasma treated material [43], each of these groups might cause the observed binding energy of a particular peak (e.g. C 1s or N 1s) to shift to varying degrees.…”

Section: X-ray Photoelectron Spectroscopy (Xps)mentioning

confidence: 99%

“…The deformation vibration of primary amines (1510-1650 cm -1 ) is observed in both spectra, but is considerably broadened in the spectrum of plasma polymerized allylamine (PPAa), which indicates the presence of alkene groups or imines. A new band appears for the polymer at 2200 cm -1 which is associated with the stretching vibration of nitrile (C≡N) groups [43,64,65]. …”

Section: Fourier-transform Infrared (Ftir) Spectroscopymentioning

confidence: 99%

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