Surface protein adhesins of Staphylococcus aureus

Foster, Timothy J.; Höök, Magnus

doi:10.1016/s0966-842x(98)01400-0

Cited by 859 publications

(676 citation statements)

References 47 publications

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“…We also included sasC, a cell surface anchor protein actively involved in biofilm attachment and maturation. 31 This transposon mutant, with ( Figure 5F,L) and without treatment ( Figure 5A,G), behaved the same as the wild type in bacterial attachment or biofilm formation. Overall, our results from the static model essentially agree with those obtained from the fluidic BioFlux system.…”

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confidence: 65%

Anti-Staphylococcal Biofilm Effects of Human Cathelicidin Peptides

Mishra

Golla

Lau

et al. 2015

ACS Med. Chem. Lett.

101

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Staphylococcus aureus can live together in the form of biofilms to avoid elimination by the host. Thus, a useful strategy to counteract bacterial biofilms is to re-engineer human antimicrobial peptide LL-37 so that it can be used as a remedy for preventing and removing biofilms. This study reports antibiofilm effects of four human cathelicidin LL-37 peptides against community-associated and hospital isolated methicillin-resistant Staphylococcus aureus (MRSA) strains. Although the intact molecule LL-37 inhibited biofilm formation at low concentrations, it did not inhibit bacterial attachment nor disrupt preformed biofilms. However, two 17-residue peptides, GF-17 and 17BIPHE2, inhibited bacterial attachment, biofilm growth, and disrupted established biofilms. An inactive peptide RI-10 was used as a negative control. Our results obtained using the S. aureus mutants in a static biofilm model are consistent with the literature obtained in a flow cell biofilm model. Because 17BIPHE2 is the most effective biofilm disruptor with desired stability to proteases, it is a promising lead for developing new anti-MRSA biofilm agents.

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mentioning

confidence: 65%

Anti-Staphylococcal Biofilm Effects of Human Cathelicidin Peptides

Mishra

Golla

Lau

et al. 2015

ACS Med. Chem. Lett.

101

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“…These molecules enable bacteria to bind to different extracellular matrix proteins with which foreign-body materials, such as sutures, are coated shortly after they are introduced into the human body. [41][42][43] The microbial characteristics of an implanted medical device, therefore, depend in the first instance on its affinity for such proteins. Concerning direct binding of bacteria to surfaces, interspecies comparison showed that pathogens tend to be hydrophobic 44 and that their hydrophobic properties decide whether bacteria preferably bind to hydrophobic or to hydrophilic surfaces.…”

Section: Discussionmentioning

confidence: 99%

“…41 In these biofilms, even ABX-resistant bacterial subpopulations may form, 51 partly because biofilms are barely penetrable for many ABXs. And even though a few remaining ABXs are not yet hindered by biofilms, such as daptomycin, 52,53 the increasing bacterial resistance rate underscores the necessity of optimizing the microbial properties of medical devices.…”

Section: Discussionmentioning

confidence: 99%

Bacterial adhesion to suture material in a contaminated wound model: Comparison of monofilament, braided, and barbed sutures

Dhom

Bloes

Peschel

et al. 2016

Journal Orthopaedic Research

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Contaminated suture material plays an important role in the physiopathology of surgical site infections. Recently, suture material has been developed characterized by barbs projecting from a monofilament base. Claimed advantages for barbed sutures are a shortened wound closure time and reduced maximum wound tension. It has also been suggested that these sutures would be advantageous microbiologically. The aim of this study was to test the microbiological characteristics of the barbed Quill in comparison to the monofilament Ethilon II and the braided sutures Vicryl and triclosan-coated Vicryl Plus. In our study, sutures were cultivated on color-change agar with Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Escherichia coli, and Pseudomonas aeruginosa and the halo size was measured. In a second study arm with longer cultivation bacterial growth was followed by antibiotic treatment. Ethilon II and Quill showed good comparable results, whereas large halos were found around Vicryl. Vicryl Plus results depended on triclosan sensitivity. After longer bacterial cultivation and antibiotic treatment, halos were up to 3.6 times smaller on Quill than on Vicryl (p < 0.001), but 1.4 times larger than on Ethilon II (p < 0.001) regarding S. aureus. Confocal microscopy analysis showed bacterial colonization between the braided filaments on Vicryl and beneath the barbs on Quill. From a microbiological perspective, barbed sutures can be recommended in aseptic surgery, but should only be used carefully in septic surgery.

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“…S. aureus is indeed one of the most important contagious mastitis-causing bacterial pathogens worldwide and is responsible for clinical and subclinical as well as chronic and acute mastitis (Radostits et al 2010;Zadoks et al 2011;Keefe 2012). S. aureus produces several putative virulence factors contributing to its pathogenicity: protein adhesins, named BMicrobial Surface Components Recognizing Adhesive Matrix Molecules^(or MSCRAMMs) interacting with host extracellular components; polysaccharide intercellular adhesins involved in the production of biofilm; other surface antigens intervening in the evasion of the innate and acquired immune responses (capsules, protein A) and secreted toxins causing cellular and tissue damages, such as haemolysins, leukocidins and enterotoxins (Foster and Höök 1998;Dinges et al 2000;Srinivasan et al 2006;Nishifuji et al 2008;Ote et al 2011;Gogoi-Tiwari et al 2015).…”

Section: Introductionmentioning

confidence: 99%

A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger

Issa

Duprez

Alambédji

et al. 2015

Trop Anim Health Prod

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Staphylococcus (S.) aureus is one of the most important pathogens causing bovine mastitis. The aim of the present work was to follow in three herds and during the 3 years the clonality of S. aureus isolated from California Mastitis Test (CMT)-positive cows at the experimental station of Toukounous (Niger) by (i) comparing their pulsed field gel electrophoresis (PFGE) fingerprints, (ii) identifying their virulotypes by PCR amplification and (iii) assessing the production of capsule and the formation of biofilm. The 88 S. aureus isolates belonged to 14 different pulsotypes, 3 of them being predominant: A (30 %), D (27 %), B (15 %). A and B pulsotypes had the highest profile similarity coefficient (94 %), while others had similarity coefficients under 60 %. Seventy-five S. aureus isolates were further studied for their virulotypes, capsular antigens and biofilm production. Most surface factor-, leukocidin-and haemolysin-, but not the enterotoxin-encoding genes were detected in the majority (>75 %) of the isolates and were evenly distributed between the A, B and D pulsotype isolates. The majority of the 72 S. aureus positive with the cap5H or cap8H PCR produced the CP5 (82 %) or the CP8 (88 %) capsular antigen, respectively. Biofilm production by the 57 icaA-positive isolates was strong for 8 isolates, moderate for 31 isolates but weak for 18 isolates, implying that the icaA gene may not be expressed in vitro by one third of the positive isolates. Similar to other studies, those results confirm that a restricted number of S. aureus clones circulate within the three herds at Toukounous and that their specific virulence-associated properties must still be further studied.

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Surface protein adhesins of Staphylococcus aureus

Cited by 859 publications

References 47 publications

Anti-Staphylococcal Biofilm Effects of Human Cathelicidin Peptides

Anti-Staphylococcal Biofilm Effects of Human Cathelicidin Peptides

Bacterial adhesion to suture material in a contaminated wound model: Comparison of monofilament, braided, and barbed sutures

A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger

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