Surface Science of DNA Adsorption onto Citrate-Capped Gold Nanoparticles

Zhang, Xu; Servos, Mark R.; Liu, Juewen

doi:10.1021/la205036p

Cited by 282 publications

(331 citation statements)

References 62 publications

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“…22,[24][25][26] The adsorption of non-thiolated DNA has also found important applications in analytical chemistry, [27][28][29][30][31][32][33][34] polymerase chain reactions, 35,36 nanoparticle synthesis, 37,38 and medicine. 37,39 We recently reported that at neutral pH, each 13 nm AuNP adsorbs just around twenty 12-mer DNAs even after overnight incubation, 40 which is consistent with the results from the literature using polyadenosine (e.g A20) as an anchoring block for DNA adsorption with the salt aging approach. 41 This number is much lower than that can be achieved using thiolated DNA (up to 130 DNAs on each 13 nm AuNP), supporting the DNA wrapping model presented in Figure 1B.…”

Section: This Document Is the Accepted Manuscript Version Of A Publissupporting

confidence: 87%

“…Cytosine (pKa = 4.2) and adenine (pKa = 3.5) can be effectively protonated at pH 3, thus decreasing the electrostatic repulsion and favoring DNA adsorption onto AuNP surfaces. The loading of FAM-T15 at pH 3 was similar to that achieved at neutral pH, 40 consistent with the fact that thymine cannot be protonated at pH 3. A 12-mer DNA with random sequence (Table 1) also showed a high loading capacity of ~93 DNA per AuNP at pH 3 ( Figure 2B).…”

Section: Based Onsupporting

confidence: 76%

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Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

et al. 2012

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The last 16 years have witnessed the landmark development of polyvalent thiolated DNA functionalized gold nanoparticles (AuNPs) possessing striking properties within the emerging field of nanobiotechnology. Many novel properties of this hybrid nanomaterial are attributed to the dense DNA shell. However, the question of whether non-thiolated polyvalent DNA-AuNP could be fabricated with high DNA density and similar properties as its thiolated counterpart has not been explored in detail.Herein, we report that by simply tuning the pH of the DNA/AuNP mixture, an ultrahigh capacity of nonthiolated DNA can be conjugated to AuNPs in a few minutes, resulting in polyvalent DNA-AuNP conjugates with cooperative melting behavior, a typical property for polyvalent thiolated DNA functionalized AuNPs. With this method, large AuNPs (e.g., 50 nm) can be functionalized to achieve colorimetric detection of sub-nM DNA. Further, this fast and stable DNA loading was employed to separate AuNPs of different size. We propose that a large fraction of the attached DNAs are adsorbed via one or a few terminal bases to afford the high loading capacity and the ability to hybridize with the complementary DNA. This discovery not only offers a time-and cost-effective way to functionalize AuNPs with a high density of non-thiolated DNA, but also provides new insights into the fundamental understanding of how DNA strands with different sequences interact with AuNPs.

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Section: This Document Is the Accepted Manuscript Version Of A Publissupporting

confidence: 87%

Section: Based Onsupporting

confidence: 76%

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

et al. 2012

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“…For metallic nanoparticles (e.g., AuNPs) and carbon-based nanomaterials (e.g., graphene oxide and carbon nanotubes), DNA adsorption is achieved mainly via base interaction. [8][9][39][40][41] For example, adding phosphate has little effect on DNA adsorbed by these materials. Many MONPs (e.g., TiO2, CeO2, ITO) adsorb DNA mainly via the phosphate 10 backbone.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Screen of Metal Oxide Nanoparticles for DNA Adsorption, Fluorescence Quenching, and Anion Discrimination

Liu

2015

ACS Appl. Mater. Interfaces

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While DNA has been quite successful in metal cation detection, anion detectioin remains challenging due to the charge repulsion. Metal oxides represent a very important class of materials, and different oxides might interact with anions differently. In this work, a comprehensive screen of common metal oxide nanoparticles (MONPs) was carried out for their ability to adsorb DNA, quench fluorescence, and release adsorbed DNA in the presence of target anions. A total of 19 MONPs were studied, including Al2O3, CeO2, CoO, Co3O4, Cr2O3, Fe2O3, Fe3O4, In2O3, ITO, Mn2O3, NiO, SiO2, SnO2, a-TiO2 (anatase), r-TiO2 (rutile), WO3, Y2O3, ZnO, ZrO2. These MONPs have different DNA adsorption affinity. Some adsorb DNA without quenching the fluorescence, while others strongly quench adsorbed fluorophores. They also display different affinity toward anions probed by DNA desorption. Finally CeO2, Fe3O4, and ZnO were used to form a sensor array to discriminate phosphate, arsenate, and arsenite from the rest using linear discriminant analysis.This study not only provides a solution for anion discrimination using DNA as a signaling molecule, but also provides insights into the interface of metal oxides and DNA.

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“…[18][19][20][21][22] We reason that this problem might be overcome by introducing more specific intermolecular forces based on the properties of the target molecules. For this purpose, DNA is an ideal model target to obtain fundamental insights since arbitrary sequences and modifications are readily accessible through chemical DNA synthesis.…”

Section: Introductionmentioning

confidence: 99%

“…For example, citrate-capped gold nanoparticles (AuNPs) strongly bind to DNA bases. [18][19][20][21][22] Negatively charged graphene oxide (GO) adsorbs DNA via hydrophobic interactions, hydrogen bonding and π-π stacking. [23][24][25] In both cases, ss-DNA adsorbs much faster than ds-DNA.…”

Section: Introductionmentioning

confidence: 99%

Rationally Designed Nucleobase and Nucleotide Coordinated Nanoparticles for Selective DNA Adsorption and Detection

et al. 2013

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Abstract.Nanomaterials for DNA adsorption are useful for sequence-specific DNA detection. Current materials for DNA adsorption employ electrostatic attraction, hydrophobic interaction, or π-π stacking, none of which can achieve sequence specificity. Specificity might be improved by involving hydrogen bonding and metal coordination. In this work, a diverse range of nucleobase/nucleotide (adenine, adenosine, ATP, AMP, and GTP) coordinated materials containing various metal ions (Au(III), Ag(I), Ce(III), Gd(III), and Tb(III)) are prepared. In most cases, nanoparticles are formed. These materials have different surface charges and positively charged particles only show non-specific DNA adsorption. Negatively charged materials give different adsorption kinetics for different DNA sequences, where complementary DNA homopolymers are adsorbed faster than other sequences. Therefore, the bases in the coordinated materials can still form base pairs with the DNA. The adsorption strength is mainly controlled by the metal ions, where Au shows the strongest adsorption while lanthanides are weaker. These materials can Wang, F., Liu, B., Huang, P.-J. J., & Liu, J. (2013). Rationally Designed Nucleobase and Nucleotide Coordinated Nanoparticles for Selective DNA Adsorption and Detection. Analytical Chemistry, 85(24), 12144-12151. https:// doi.org/10.1021/ac4033627 2 be used as sensors for DNA detection and can also deliver DNA into cells with no detectable toxicity. By tuning the nanoparticle formulation, enhanced detection can be achieved. This study is an important step towards rational design of materials to achieve specific interactions between biomolecules and synthetic nanoparticle surfaces. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see

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Surface Science of DNA Adsorption onto Citrate-Capped Gold Nanoparticles

Cited by 282 publications

References 62 publications

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

Comprehensive Screen of Metal Oxide Nanoparticles for DNA Adsorption, Fluorescence Quenching, and Anion Discrimination

Rationally Designed Nucleobase and Nucleotide Coordinated Nanoparticles for Selective DNA Adsorption and Detection

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