Surface treatments and functionalization of metal‐ceramic membranes for improved enzyme immobilization performance

Zeuner, Birgitte; Ovtar, Simona; Persson, Anders; Foghmoes, Søren Preben Vagn; Berendt, Kasper; Ma, Nicolaj; Kaiser, Andreas; Negra, Michela Della; Pinelo, Manuel

doi:10.1002/jctb.6278

Cited by 17 publications

(15 citation statements)

References 50 publications

(141 reference statements)

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“…Considering novel ceramic membrane materials, the improved microstructure obtained by yttria (Y 2 O 3 ) impregnation had a significant effect on enzyme loading yield and activity. This indicates the potential of this surface modification method and of these metal-supported ceramic membranes in enzyme immobilization [23]. Thus, yttria stabilized ceramic membrane show less biofouling, which reduces the cake layer resistance due to biofouling.…”

Section: Introductionmentioning

confidence: 78%

New Insights into the Microbial Diversity of Cake Layer in Yttria Composite Ceramic Tubular Membrane in an Anaerobic Membrane Bioreactor (AnMBR)

Nilusha

Wei

2021

Membranes

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Cake layer formation is an inevitable challenge in membrane bioreactor (MBR) operation. The investigations on the cake layer microbial community are essential to control biofouling. This work studied the bacterial and archaeal communities in the cake layer, the anaerobic sludge, and the membrane cleaning solutions of anaerobic membrane bioreactor (AnMBR) with yttria-based ceramic tubular membrane by polymerase chain reaction (PCR) amplification of 16S rRNA genes. The cake layer resistance was 69% of the total membrane resistance. Proteins and soluble microbial by-products (SMPs) were the dominant foulants in the cake layer. The pioneering archaeal and bacteria in the cake layer were mostly similar to those in the anaerobic bulk sludge. The dominant biofouling bacteria were Proteobacteria, Bacteroidetes, Firmicutes, and Chloroflexi and the dominant archaeal were Methanosaetacea and Methanobacteriacea at family level. This finding may help to develop antifouling membranes for AnMBR treating domestic wastewater.

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Section: Introductionmentioning

confidence: 78%

New Insights into the Microbial Diversity of Cake Layer in Yttria Composite Ceramic Tubular Membrane in an Anaerobic Membrane Bioreactor (AnMBR)

Nilusha

Wei

2021

Membranes

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“…To achieve the efficient immobilization of enzymes on ceramic membranes, the membrane surface needs to be modified by linker molecules [15,16,[21][22][23]. Hoog Antink et al [16] modified yttria-stabilized zirconia (YSZ) capillary membranes with 3-(triethoxysilyl) propylsuccinic anhydride (TESPSA) or (3-aminopropyl) triethoxysilane (APTES) to investigate the impact of membrane surface modification on the enzyme (protease subtilisin A) attachment and the catalytic performance in the protein hydrolysis process.…”

Section: Introductionmentioning

confidence: 99%

“…The type of linker (silane) was of great importance for the initial adsorptive enzyme binding, the formation of covalent bonds, and the specific enzyme activity [16]. Zeuner et al [21] fabricated YSZ ceramic membranes on a stainless steel metal support layer for the immobilization of alcohol dehydrogenase (ADH). In their work, ADH was immobilized on ceramic membranes by using two techniques, polyethyleneimine (PEI) coating, or APTES grafting, followed by glutaraldehyde (GA) activation and the consequent covalent immobilization of enzymes.…”

Section: Introductionmentioning

confidence: 99%

Bioconjugation Strategy for Ceramic Membranes Decorated with Candida Antarctica Lipase B—Impact of Immobilization Process on Material Features

et al. 2022

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A strategy for the bioconjugation of the enzyme Candida antarctica lipase B onto titania ceramic membranes with varied pore sizes (15, 50, 150, and 300 kDa) was successfully performed. The relationship between the membrane morphology, i.e.,the pore size of the ceramic support, and bioconjugation performance was considered. Owing to the dimension of the enzyme (~33 kDa), the morphology of the ceramics allowed (50, 150, and 300 kDa) or did not allow (15 kDa) the entrance of the enzyme molecules into the porous structure. Such a strategy made it possible to better understand the changes in the material (morphology) and physicochemical features (wettability, adhesiveness, and surface charge) of the samples, which were systematically examined. The silane functionalization and enzyme immobilization were accomplished via the covalent route. The samples were characterized after each stage of the modification, which was very informative from the material point of view. As a consequence of the modification, significant changes in the contact angle, roughness, adhesion, and zeta potential were observed. For instance, for the 50 kDa membrane, the contact angle increased from 29.1 ± 1.5° for the pristine sample to 72.3 ± 1.5° after silane attachment; subsequently, it was reduced to 57.2 ± 1.5° after the enzyme immobilization. Finally, the contact angle of the bioconjugated membrane used in the enzymatic process rose to 92.9 ± 1.5°. By roughness (Sq) controlling, the following amendments were noticed: for the pristine 50 kDa membrane, Sq = 1.87 ± 0.21 µm; after silanization, Sq = 2.33 ± 0.30 µm; after enzyme immobilization, Sq = 2.74 ± 0.26 µm; and eventually, after the enzymatic process, Sq = 2.37 ± 0.27 µm. The adhesion work of the 50 kDa samples was equal to 136.41 ± 2.20 mN m−1 (pristine membrane), 94.93 ± 2.00 mN m−1 (with silane), 112.24 ± 1.90 mN m−1 (with silane and enzyme), and finally, 69.12 ± 1.40 mN m−1 (after the enzymatic process). The materials and physicochemical features changed substantially, particularly after the application of the membrane in the enzymatic process. Moreover, the impact of ceramic material morphology on the zeta potential value is here presented for the first time. With an increase in the ceramic support cut-off, the amount of immobilized lipase rose, but the specific productivity was higher for membranes possessing smaller pores, owing to the higher grafting density. For the enzymatic process, two modes of accomplishment were selected, i.e., stirred-tank and cross-flow. The latter method was characterized by a much higher effectiveness, with a resulting productivity equal to 99.7 and 60.3 µmol h−1 for the 300 and 15 kD membranes, respectively.

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“…Immobilization technology helps to enhance the stability of an enzyme and solves the reusability problem. [4][5][6] Laccase is an enzyme that has a wide range of substrates. It can catalyze phenolic, non-phenolic, and most of the aromatic compounds.…”

Section: Introductionmentioning

confidence: 99%

Surface and stability analysis of immobilized laccase on poly(ethylene terephthalate) grafted maleic anhydride nanofiber mat

Syukri

Mohamad

Rahman

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND: A poly(ethylene terephthalate) (PET) based carrier was used for enzyme immobilization due to its good physical and mechanical properties. However, its high hydrophobicity and chemically inactive properties hindered its capability as an enzyme carrier. This study developed a PET-based carrier by grafting PET with maleic anhydride (MAH) before electrospinning. This carrier was used to immobilize laccase and characterized based on its stability after immobilization.RESULTS: The highest laccase activity recovery for laccase immobilized on PET-g-MAH nanofiber mat was 59.17% using covalent bonding, followed by the crosslinking method. The static water contact angle of PET-g-MAH nanofiber mats was reduced from 129 ± 8 o to 109 ± 12 o . Based on the Wenzel model, the reduction was due to the decrease in surface roughness of the hydrophobic surface. Besides, the immobilized laccase also withstood high temperature up to 60 °C and retained about 29.22% ± 5.06% of its initial activity after 30 days of storage and repeated use.CONCLUSION: Laccase immobilization on PET-g-MAH nanofiber has good stability (thermal, storage and reusability) and is recommended to be implemented in the industries.

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Surface treatments and functionalization of metal‐ceramic membranes for improved enzyme immobilization performance

Cited by 17 publications

References 50 publications

New Insights into the Microbial Diversity of Cake Layer in Yttria Composite Ceramic Tubular Membrane in an Anaerobic Membrane Bioreactor (AnMBR)

New Insights into the Microbial Diversity of Cake Layer in Yttria Composite Ceramic Tubular Membrane in an Anaerobic Membrane Bioreactor (AnMBR)

Bioconjugation Strategy for Ceramic Membranes Decorated with Candida Antarctica Lipase B—Impact of Immobilization Process on Material Features

Surface and stability analysis of immobilized laccase on poly(ethylene terephthalate) grafted maleic anhydride nanofiber mat

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