Surfactant Protein a in Rabbit Sinus and Middle Ear Mucosa

Dutton, Jay M.; Goss, Kelli L.; Khubchandani, Kavita R.; Shah, Chirag; Smith, Richard J.H.; Snyder, Jeanne M.

doi:10.1177/000348949910801001

Cited by 40 publications

(38 citation statements)

References 27 publications

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“…Recently, Paananen et al (31) described the expression of surfactant protein (SP) A and SP-D in the epithelial lining of the ET. SP-A has also been detected in the middle ear mucosa (8). These data suggest that innate immune responses might play an important role in the protection of the ET against infection.…”

mentioning

confidence: 71%

Surfactant proteins A and D in Eustachian tube epithelium

Paananen

Sormunen

Glumoff

et al. 2001

American Journal of Physiology-Lung Cellular and Molecular Phys

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collectin; innate immunity; in situ hybridization; immunoelectron microscopy THE EUSTACHIAN TUBE (ET) develops embryonically from the first pharyngeal pouch and connects the upper respiratory tract to the middle ear. The ET protects the middle ear from excessive fluctuations of atmospheric pressure, serves as a clearance tract, and may protect the middle ear against the invasion of microbes and other noxious agents from the airways (3). Infections in the middle ear are very common, affecting nearly all children at least once, and ϳ20% of children have recurrent otitis media (ROM). Dysfunction of the ET is considered to be the principal pathogenetic factor in the susceptibility to ROM, which is largely determined by hereditary factors (9). However, the mechanisms and the genes involved remain unknown. Evidence of the presence of a surface tension-lowering substance in the ET has been found (2). Recently, Paananen et al. (31) described the expression of surfactant protein (SP) A and SP-D in the epithelial lining of the ET. SP-A has also been detected in the middle ear mucosa (8). These data suggest that innate immune responses might play an important role in the protection of the ET against infection.

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mentioning

confidence: 71%

Surfactant proteins A and D in Eustachian tube epithelium

Paananen

Sormunen

Glumoff

et al. 2001

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Previous immunohistochemical analyses have revealed that SP-A and SP-D are prominently expressed in type II alveolar pneumocytes [38] and that KL-6 is expressed in type II alveolar pneumocytes and in bronchiolar epithelial and bronchial gland cells [39]. Other studies have detected SP-A also in the bronchiolar, tracheal, sinus and middle ear epithelium [17,40,41,42,43]. This study indicates that sputum levels of SP-A, SP-D and KL-6 were elevated in the induced sputum of COPD patients compared to patients with prolonged cough.…”

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confidence: 99%

Levels of Surfactant Proteins A and D and KL-6 Are Elevated in the Induced Sputum of Chronic Obstructive Pulmonary Disease Patients: A Sequential Sputum Analysis

et al. 2011

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Background: Recent clinical studies have suggested that serum surfactant protein (SP) A, SP-D and Krebs von den Lungen-6 (KL-6) are potential biomarkers for interstitial lung diseases. Serum levels of SP-A and SP-D have also been found to be elevated in chronic obstructive pulmonary disease (COPD), but their significance has not been evaluated or compared in induced sputum samples obtained directly from the airways. Objective: A sequential sputum analysis was conducted to assess the value of SP-A, SP-D and KL-6 levels in COPD. Methods: The study material consisted of induced sputum samples from 61 subjects, 28 with COPD and 33 with prolonged cough (cough lasting >3 weeks and normal spirometry). Sputum was collected in 3 fractions (3 periods of 5 min each). Sputum levels of these proteins were measured, and receiver operating characteristic curve analysis was carried out to evaluate the sensitivity, specificity and area under the curve (AUC) for each fraction. Results: The levels of SP-A, SP-D and KL-6 were higher in patients with COPD than in those with prolonged cough in each of the fractions. Sputum levels of these proteins correlated inversely with obstruction and positively with ageing, smoking history, sputum macrophages and eosinophils. Sputum fractionation had a relatively minor effect on the levels and AUC of these proteins. Conclusion: Sequential sputum analysis from 3 consecutive fractions indicated a significant difference in the levels of SP-A, SP-D and KL-6 between COPD and prolonged cough. However, sputum fractionation itself had a relatively minor effect on the levels of these proteins.

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“…The observation that a phospholipid-rich surface lining is not unique to the lung alveoli suggested that other organs might also express SPs. To date, the expression of SPs has been detected in the gastric and intestinal mucosa (1,7), joints (1), peritoneum (1), pericardium (1,7), skin (12), middle ear (2,11), Eustachian tubes (13,14), and maxillary sinus mucosa (2). In addition, surfactant-like materials have been identified in the tongue papillae and oral epithelium (1).…”

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confidence: 99%

Expression and localization of surfactant proteins in human nasal epithelium

Kim¹,

Kim²,

Rha³

et al. 2007

American Journal of Physiology-Lung Cellular and Molecular Phys

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Surfactant proteins (SPs), designated SP-A, SP-B, SP-C, and SP-D, play an important role in surfactant metabolism and host defense mechanisms in the lung. This study investigates expression of the different SP types in human nasal mucosa and cultured normal human nasal epithelial (NHNE) cells and whether the expression of SP mRNA is influenced by the degree of mucociliary differentiation. RT-PCR was performed with mRNA from cultured NHNE cells and nasal mucosa. Immunohistochemical staining for SPs was performed on nasal mucosa specimens. Western blot analysis was performed on cell lysates from cultured NHNE cells. SP-A2, SP-B, and SP-D mRNAs were expressed in normal NHNE cells and human nasal mucosa. SPs were localized in ciliated cells of the surface epithelium and serous acini of the submucosal glands. SP-A, SP-B, and SP-D proteins were expressed in cultured NHNE cells. The degree of mucociliary differentiation influenced expression of the SP gene. We demonstrate that SP-A, SP-B, and SP-D are expressed in human nasal mucosa and cultured NHNE cells. Further study of the functional role of SPs in the upper airway is required.

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Surfactant Protein a in Rabbit Sinus and Middle Ear Mucosa

Cited by 40 publications

References 27 publications

Surfactant proteins A and D in Eustachian tube epithelium

Surfactant proteins A and D in Eustachian tube epithelium

Levels of Surfactant Proteins A and D and KL-6 Are Elevated in the Induced Sputum of Chronic Obstructive Pulmonary Disease Patients: A Sequential Sputum Analysis

Expression and localization of surfactant proteins in human nasal epithelium

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