Surfactant Treatment for Efficient Gene Detection of Enteric Viruses and Indicators in Surface Water Concentrated by Ultrafiltration

Hata, Akihiko; Meuchi, Yuno; Liu, Miaomiao; Torii, Shotaro; Katayama, Hiroyuki

doi:10.1007/s12560-022-09543-y

Cited by 5 publications

(2 citation statements)

References 57 publications

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“…The sample in the 20 L polyethylene container was concentrated by hollow fiber ultrafiltration (HFUF) cartridge (APS-25SA; Asahi Kasei Medical Co., Osaka, Japan) and following centrifugation and polyethylene glycol (PEG) precipitation, as previously described [37][38][39]. Prior to the sample filtration, the HFUF was blocked by circulating 200 mL of fetal bovine serum (FBS) in the cartridge for 2 minutes and incubated overnight.…”

Section: Concentration Of Microbesmentioning

confidence: 99%

Applicability of F-specific bacteriophage subgroups, PMMoV and crAssphage as indicators of source specific fecal contamination and viral inactivation in rivers in Japan

et al. 2023

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To date, several microbes have been proposed as potential source-specific indicators of fecal pollution. 16S ribosomal RNA gene markers of the Bacteroidales species are the most widely applied due to their predominance in the water environment and source specificity. F-specific bacteriophage (FPH) subgroups, especially FRNA phage genogroups, are also known as potential source-specific viral indicators. Since they can be quantified by both culture-based and molecular assays, they may also be useful as indicators for estimating viral inactivation in the environment. Pepper mild mottle virus (PMMoV) and crAssphage, which are frequently present in human feces, are also potentially useful as human-specific indicators of viral pollution. This study aimed to evaluate the applicability of FPH subgroups, PMMoV, and crAssphage as indicators of source-specific fecal contamination and viral inactivation using 108 surface water samples collected at five sites affected by municipal and pig farm wastewater. The host specificity of the FPH subgroups, PMMoV, and crAssphage was evaluated by principal component analysis (PCA) along with other microbial indicators, such as 16S ribosomal RNA gene markers of the Bacteroidales species. The viabilities (infectivity indices) of FRNA phage genogroups were estimated by comparing their numbers determined by infectivity-based and molecular assays. The PCA explained 58.2% of the total information and classified microbes into three groups: those considered to be associated with pig and human fecal contamination and others. Infective and gene of genogroup IV (GIV)-FRNA phage were assumed to be specific to pig fecal contamination, while the genes of GII-FRNA phage and crAssphage were identified to be specific to human fecal contamination. However, PMMoV, infective GI-FRNA phage, and FDNA phage were suggested to not be specific to human or pig fecal contamination. FRNA phage genogroups, especially the GIV-FRNA phage, were highly inactivated in the warm months in Japan (i.e., July to November). Comparing the infectivity index of several FRNA phage genogroups or other viruses may provide further insight into viral inactivation in the natural environment and by water treatments.

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Section: Concentration Of Microbesmentioning

confidence: 99%

Applicability of F-specific bacteriophage subgroups, PMMoV and crAssphage as indicators of source specific fecal contamination and viral inactivation in rivers in Japan

et al. 2023

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“…[ 1 ] The key to averting bacterial contamination and identifying its source lies in timely and effective detection. [ 2 ] Traditional bacterial detection methods, such as culture counting, [ 3 ] enzyme‐linked immunosorbent assay, [ 4 ] and polymerase chain reaction, [ 5 ] offer high sensitivity, precise identification, and quantification. In addition, biosensors based on surface‐enhanced Raman scattering (SERS), [ 6 ] electrochemistry, [ 7 ] fluorescence, [ 8 ] and colorimetry [ 9 ] have achieved exciting results in improving detection limits and shortening detection time.…”

Section: Introductionmentioning

confidence: 99%

Seamless Integration of Rapid Separation and Ultrasensitive Detection for Complex Biological Samples Using Multistage Annular Functionalized Carbon Nanotube Arrays

Li,

Zhang,

Jiao

et al. 2024

Advanced Materials

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Efficient separation, enrichment, and detection of bacteria in diverse media are pivotal for identifying bacterial diseases and their transmission pathways. However, conventional bacterial detection methods that split the separation and detection steps are plagued by prolonged processing times, high costs, and intricate procedures that hinder the advancement of pathogen detection techniques. Herein, we introduce a multistage annular‐functionalized carbon nanotube array device designed for the seamless integration of complex biological sample separation and multi‐marker detection. This device resorted to the super‐smooth fluidity of the liquid sample in the carbon nanotubes interstice through rotation assistance, achieving the ability to quickly separate impurities and capture biomarkers (1 mL sample cost time of 2.5 s). Fluid dynamics simulations showed that the reduction of near‐surface hydrodynamic resistance drives the capture of bacteria and related biomarkers on the functionalized surface of carbon nanotube in sufficient time. When further assembled as an even detection device, it exhibited fast detection (<30 min), robust linear correlation (101–107 colony‐forming units [CFU]/mL, R2 = 0.997), ultrasensitivity (limit of detection = 1.7 CFU/mL), and multi‐target detection (Staphylococcus aureus, extracellular vesicles, and enterotoxin proteins). Collectively, our material and system offer an expanded platform for real‐time diagnostics, enabling integrated rapid separation and detection of various disease biomarkers. The device has significant potential applications in fields such as emergent infectious disease diagnosis and treatment, infection source tracing, and environmental monitoring.This article is protected by copyright. All rights reserved

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Activity- and gene-based quantification of enteric viruses, F- specific RNA phage genogroups, pepper mild mottle virus, and Escherichia coli in surface water

Hata,

Meuchi,

Liu

et al. 2023

Science of The Total Environment

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Surfactant Treatment for Efficient Gene Detection of Enteric Viruses and Indicators in Surface Water Concentrated by Ultrafiltration

Cited by 5 publications

References 57 publications

Applicability of F-specific bacteriophage subgroups, PMMoV and crAssphage as indicators of source specific fecal contamination and viral inactivation in rivers in Japan

Applicability of F-specific bacteriophage subgroups, PMMoV and crAssphage as indicators of source specific fecal contamination and viral inactivation in rivers in Japan

Seamless Integration of Rapid Separation and Ultrasensitive Detection for Complex Biological Samples Using Multistage Annular Functionalized Carbon Nanotube Arrays

Activity- and gene-based quantification of enteric viruses, F- specific RNA phage genogroups, pepper mild mottle virus, and Escherichia coli in surface water

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