Surgical Approach of an Early Mammalian Embryo: The Rabbit Model

Beaudoın, Sylvie; Simon, L; Simeoni, J.; Sacquin, P; Bargy, F.

doi:10.1159/000020811

Cited by 4 publications

(2 citation statements)

References 29 publications

(18 reference statements)

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“…The procedure may be biochemical [3,4], surgical [5][6][7][8][9], or even magnetic [10]. Nevertheless, especially in embryos, the critical point is to choose the appropriate developmental stage to perform a study, in order to obtain the most satisfactory results [5,11].…”

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confidence: 99%

Developmental Stages in the Rabbit Embryo: Guidelines to Choose an Appropriate Experimental Model

Beaudoın

Barbet²,

Bargy

2003

Fetal Diagn Ther

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Researchers involved in the field of congenital malformations are often forced to work on an animal model. Both accurate description of its normal development and comparative staging with human development will be mandatory. To complete the lacking medical literature, we herein provide such data for the rabbit model. Sampled rabbit embryos were staged using the Carnegie criteria, in order first to determine if they were consistent with the rabbit developmental pattern, and second to compare this pattern with the human one. Our results show a suitable comparison of rabbits and humans in early developmental stages, except for the neural growth.

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Developmental Stages in the Rabbit Embryo: Guidelines to Choose an Appropriate Experimental Model

Beaudoın

Barbet²,

Bargy

2003

Fetal Diagn Ther

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“…In this respect, the midgestational rabbit may represent a good model for first-trimester human pregnancy where the exocoelomic space is only present up to 12 weeks of gestation. The rabbit has been successfully used in the field of fetal surgical research, as early as the embryonic period [7]. Additionally, it has been used in the study of in utero somatic gene transfer [8,9] and fetoscopy [10,11].…”

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Biochemical Differentiation of Gestational Compartments in the Midgestational Fetal Rabbit

Devlieger¹,

Gratacós²,

Wu³

et al. 2001

Fetal Diagn Ther

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Objectives: The fetal rabbit at midgestation is increasingly being used as a model in fetal diagnosis and therapy. In this study, we aimed to establish a reliable method for identification of the origin of sampled extra-embryonic fluids based on selected biochemical components. Methods: In 6 pregnant does at 22 days of gestation, 18 gestational sacs were sampled for amniotic, allantoic and exocoelomic fluid. These fluids, as well as matching maternal and fetal blood samples, were assayed for levels of sodium, potassium, chloride, bicarbonate, total protein, alkaline phosphatase, γ-glutamyl transferase and progesterone. Results: Levels of sodium and potassium were, respectively, lower and higher in the allantoic fluid when compared to other extra-embryonic spaces. Amniotic fluid had a significantly lower total protein content and higher level of alkaline phosphatase when compared to the exocoelomic fluid. Significant levels of progesterone could only be detected in maternal blood. Conclusions: In the midgestational rabbit, a combined assay of potassium, alkaline phosphatase and progesterone can determine the gestational cavity of origin of the sampled fluid. The obtained gradients for these markers suggest compartment-specific production and/or inter-cavity transfer mechanisms.

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