The research aims to examine the effect of irradiated chitosan (ICh) on enzyme levels (SGPT, SGOT, and Gamma Transpeptidase) in the starter phase of Sentul chickens and its physiological conditions, as a result of fungal contamination from the environment (specifically from feed ingredients). This was conducted in the Poultry Production Laboratory, and the sample analysis was performed at the Lab of Animal Physiology and Biochemistry, Padjadjaran University. The samples consisted of 100 Sentul chickens reared from 0-8 weeks, and the method used was a completely randomized design (CRD) with 4 treatments and 5 replications. The treatments were K0 = basal diet (BD) without ICh, K1 = BD + 300 ppm ICh, K2 = BD + 350 ppm ICh, K3 = BD + 400 ppm ICh. The results showed that ICh was significantly different (P < 0.05) on SGPT, SGOT, and Gamma Transpeptidase levels. Therefore, it was concluded that the administration of 400 ppm ICh in the feed effectively enhanced the liver status of Sentul chickens in the starter phase.